wee1-b

Functionⁱ

Acts as a zygotic negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed cdk1 before the onset of mitosis by mediating phosphorylation of cdk1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed cdk1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-cdk1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric cdk1 does not occur.1 Publication

Catalytic activityⁱ

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	277	ATPPROSITE-ProRule annotation	1
Active siteⁱ	375	Proton acceptorPROSITE-ProRule annotation	1
Metal bindingⁱ	380	Magnesium; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	412	Magnesium; via carbonyl oxygenBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	254 – 262	ATPPROSITE-ProRule annotation		9

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
magnesium ion binding Source: InterPro
non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

Complete GO annotation...

GO - Biological processⁱ

cell division Source: UniProtKB-KW
mitotic cell cycle Source: InterPro
negative regulation of cell cycle
Source: CACAO
Inferred from direct assayⁱ
- 17961226

Complete GO annotation...

Keywordsⁱ

Molecular function	Kinase, Transferase, Tyrosine-protein kinase
Biological process	Cell cycle, Cell division, Mitosis
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Wee1-like protein kinase 1-B (EC:2.7.10.2) Alternative name(s): Zygotic wee1-like protein kinase 1B Short name: Xe-Wee1B Short name: XeWee1B
Gene namesⁱ	Name:wee1-b
Organismⁱ	Xenopus laevis (African clawed frog)
Taxonomic identifierⁱ	8355 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Organism-specific databases

Xenbaseⁱ	XB-GENE-6254033. wee1.

Xenbaseⁱ

XB-GENE-6254033. wee1.

Subcellular locationⁱ

Nucleus By similarity

GO - Cellular componentⁱ

nucleus Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	181 – 183	NIN → AAA: Impairs without abolishing ability to phosphorylate cdk1. 1 Publication		3
Mutagenesisⁱ	186	T → A: Abolishes kinase activity inhibition during M-phase. Does not affect ability to phosphorylate cdk1. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000409523	1 – 595	Wee1-like protein kinase 1-BAdd BLAST		595

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	186	Phosphothreonine; by cdk11 Publication		1

Post-translational modificationⁱ

Phosphorylation at Thr-186 during M-phase by cdk1 inhibits the kinase activity and leads to interaction with pin1.1 Publication

Keywords - PTMⁱ

Phosphoprotein

PTM databases

iPTMnetⁱ	Q8QGV2.

iPTMnetⁱ

Q8QGV2.

Expressionⁱ

Tissue specificityⁱ

Zygotically expressed. Present in oocytes and postgastrula embryos (at least until the tailbud stage). Expression begins at the midblastula stage and increases after the early gastrula stage.1 Publication

Interactionⁱ

Subunit structureⁱ

Interacts (when phosphorylated at Thr-186) with pin1.1 Publication

Protein-protein interaction databases

BioGridⁱ	100680. 1 interactor.
Database of interacting proteins More...DIPⁱ	DIP-60878N.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8QGV2.
SMRⁱ	Q8QGV2.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	248 – 518	Protein kinasePROSITE-ProRule annotationAdd BLAST		271

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	526 – 563	Sequence analysisAdd BLAST		38

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	69 – 75	Poly-Glu		7

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

HOVERGENⁱ	HBG005050.
KEGG Orthology (KO) More...KOⁱ	K06632.

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. IPR017164. Wee1-like_protein_kinase.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00069. Pkinase. 1 hit.
PIRSFⁱ	PIRSF037281. Wee1-like_protein_kinase. 1 hit.
SMARTⁱ	View protein in SMART SM00220. S_TKc. 1 hit.
SUPFAMⁱ	SSF56112. SSF56112. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q8QGV2-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNVQPRNMNV QPRNMNVQPV RHKLFFSDTD EEEEDGHSTG EDSAFQESDS 
        60         70         80         90        100
PVSRQREKQE GKPPGGTWEE LEEEEGFGSS PIKSPGDFFM SDSPSYRQLA 
       110        120        130        140        150
PASPTRSPQG PTSPIPECPG TPPHKTFRKL RLFDTPHTPK SLLSKARGIG 
       160        170        180        190        200
SSALRFRGGT LFREAEKAPK PEFVYSTPQV NINPFTPDSL EIQSSAGLCR 
       210        220        230        240        250
GRKRALLNDS CGEDMEGSDC ELEDEDIRPA KRIPITESNM KSRYATEFHE 
       260        270        280        290        300
LEKIGSGEFG SVFKCVKRLD GCIYAIKRSK KPLAGSVDEQ NALREVYAHA 
       310        320        330        340        350
VLGQHPHVVR YYSAWAEDDH MLIQNEYCNG GSLSDVISEN YRTMQYFTEP 
       360        370        380        390        400
ELKDLLLQVA RGLKYIHSMS LVHMDIKPSN IFISRTTLPN TAVEEADDEE 
       410        420        430        440        450
CGSGKVIYKI GDLGHVTRVS SPQVEEGDSR FLANEVLQEN YTHLAKADIF 
       460        470        480        490        500
ALALTVWSAA GAEPFPTNGD QWHEIRQGKL PRVPQLLSQE FVDLIKLMIS 
       510        520        530        540        550
PDPEKRPSSV ALVKHSVLLS ASRKSAEQLR IELDAEKFKN ALLQKELKKA 
       560        570        580        590 
QIAKAAAEER AHFPDRIATR STTQNNRTTR LIGKKMNRSV SLTIY

Length:595

Mass (Da):66,600

Last modified:June 1, 2002 - v1

Checksum:ⁱ4E90E7FDA341CB1B

GO

Sequence cautionⁱ

The sequence AAH82404 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	67	T → S in AAH82404 (Ref. 2) Curated		1
Sequence conflictⁱ	367	H → Y in AAH82404 (Ref. 2) Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB071983 mRNA. Translation: BAB86797.1. BC082404 mRNA. Translation: AAH82404.1. Different initiation.
NCBI Reference Sequences More...RefSeqⁱ	NP_001084186.1. NM_001090717.1.
UniGeneⁱ	Xl.9986.

Genome annotation databases

GeneIDⁱ	399355.
KEGGⁱ	xla:399355.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB071983 mRNA. Translation: BAB86797.1. BC082404 mRNA. Translation: AAH82404.1. Different initiation.
NCBI Reference Sequences More...RefSeqⁱ	NP_001084186.1. NM_001090717.1.
UniGeneⁱ	Xl.9986.

3D structure databases

ProteinModelPortalⁱ	Q8QGV2.
SMRⁱ	Q8QGV2.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	100680. 1 interactor.
Database of interacting proteins More...DIPⁱ	DIP-60878N.

PTM databases

iPTMnetⁱ	Q8QGV2.

iPTMnetⁱ

Q8QGV2.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	399355.
KEGGⁱ	xla:399355.

Organism-specific databases

CTDⁱ	399355.
Xenbaseⁱ	XB-GENE-6254033. wee1.

Phylogenomic databases

HOVERGENⁱ	HBG005050.
KEGG Orthology (KO) More...KOⁱ	K06632.

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. IPR017164. Wee1-like_protein_kinase.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00069. Pkinase. 1 hit.
PIRSFⁱ	PIRSF037281. Wee1-like_protein_kinase. 1 hit.
SMARTⁱ	View protein in SMART SM00220. S_TKc. 1 hit.
SUPFAMⁱ	SSF56112. SSF56112. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	WEE1B_XENLA
Accessionⁱ	Q8QGV2Primary (citable) accession number: Q8QGV2 Secondary accession number(s): Q641D3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
	Last sequence update:	June 1, 2002
	Last modified:	July 5, 2017
	This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Documents

SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8QGV2 (WEE1B_XENLA)

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Wee1-like protein kinase 1-B

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Coiled coil

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ