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Q8QGV2 (WEE1B_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Wee1-like protein kinase 1-B

EC=2.7.10.2
Alternative name(s):
Zygotic wee1-like protein kinase 1B
Short name=Xe-Wee1B
Short name=XeWee1B
Gene names
Name:wee1-b
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length595 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a zygotic negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed cdk1 before the onset of mitosis by mediating phosphorylation of cdk1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed cdk1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-cdk1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric cdk1 does not occur. Ref.1

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Interacts (when phosphorylated at Thr-186) with pin1. Ref.3

Subcellular location

Nucleus By similarity.

Tissue specificity

Zygotically expressed. Present in oocytes and postgastrula embryos (at least until the tailbud stage). Expression begins at the midblastula stage and increases after the early gastrula stage. Ref.1

Post-translational modification

Phosphorylation at Thr-186 during M-phase by cdk1 inhibits the kinase activity and leads to interaction with pin1.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAH82404.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 595595Wee1-like protein kinase 1-B
PRO_0000409523

Regions

Domain248 – 518271Protein kinase
Nucleotide binding254 – 2629ATP By similarity
Coiled coil526 – 56338 Potential
Compositional bias69 – 757Poly-Glu

Sites

Active site3751Proton acceptor By similarity
Metal binding3801Magnesium; via carbonyl oxygen By similarity
Metal binding4121Magnesium; via carbonyl oxygen By similarity
Binding site2771ATP By similarity

Amino acid modifications

Modified residue1861Phosphothreonine; by cdk1 Ref.3

Experimental info

Mutagenesis181 – 1833NIN → AAA: Impairs without abolishing ability to phosphorylate cdk1. Ref.3
Mutagenesis1861T → A: Abolishes kinase activity inhibition during M-phase. Does not affect ability to phosphorylate cdk1. Ref.3
Sequence conflict671T → S in AAH82404. Ref.2
Sequence conflict3671H → Y in AAH82404. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8QGV2 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 4E90E7FDA341CB1B

FASTA59566,600
        10         20         30         40         50         60 
MNVQPRNMNV QPRNMNVQPV RHKLFFSDTD EEEEDGHSTG EDSAFQESDS PVSRQREKQE 

        70         80         90        100        110        120 
GKPPGGTWEE LEEEEGFGSS PIKSPGDFFM SDSPSYRQLA PASPTRSPQG PTSPIPECPG 

       130        140        150        160        170        180 
TPPHKTFRKL RLFDTPHTPK SLLSKARGIG SSALRFRGGT LFREAEKAPK PEFVYSTPQV 

       190        200        210        220        230        240 
NINPFTPDSL EIQSSAGLCR GRKRALLNDS CGEDMEGSDC ELEDEDIRPA KRIPITESNM 

       250        260        270        280        290        300 
KSRYATEFHE LEKIGSGEFG SVFKCVKRLD GCIYAIKRSK KPLAGSVDEQ NALREVYAHA 

       310        320        330        340        350        360 
VLGQHPHVVR YYSAWAEDDH MLIQNEYCNG GSLSDVISEN YRTMQYFTEP ELKDLLLQVA 

       370        380        390        400        410        420 
RGLKYIHSMS LVHMDIKPSN IFISRTTLPN TAVEEADDEE CGSGKVIYKI GDLGHVTRVS 

       430        440        450        460        470        480 
SPQVEEGDSR FLANEVLQEN YTHLAKADIF ALALTVWSAA GAEPFPTNGD QWHEIRQGKL 

       490        500        510        520        530        540 
PRVPQLLSQE FVDLIKLMIS PDPEKRPSSV ALVKHSVLLS ASRKSAEQLR IELDAEKFKN 

       550        560        570        580        590 
ALLQKELKKA QIAKAAAEER AHFPDRIATR STTQNNRTTR LIGKKMNRSV SLTIY 

« Hide

References

« Hide 'large scale' references
[1]"The existence of two distinct Wee1 isoforms in Xenopus: implications for the developmental regulation of the cell cycle."
Okamoto K., Nakajo N., Sagata N.
EMBO J. 21:2472-2484(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[3]"Mechanism for inactivation of the mitotic inhibitory kinase Wee1 at M phase."
Okamoto K., Sagata N.
Proc. Natl. Acad. Sci. U.S.A. 104:3753-3758(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT THR-186, INTERACTION WITH PIN1, MUTAGENESIS OF 181-ASN--ASN-183 AND THR-186.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB071983 mRNA. Translation: BAB86797.1.
BC082404 mRNA. Translation: AAH82404.1. Different initiation.
RefSeqNP_001084186.1. NM_001090717.1.
UniGeneXl.9986.

3D structure databases

ProteinModelPortalQ8QGV2.
SMRQ8QGV2. Positions 240-518.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid100680. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID399355.
KEGGxla:399355.

Organism-specific databases

CTD399355.
XenbaseXB-GENE-6254033. wee1.

Phylogenomic databases

HOVERGENHBG005050.
KOK06632.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameWEE1B_XENLA
AccessionPrimary (citable) accession number: Q8QGV2
Secondary accession number(s): Q641D3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families