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Protein

Three-finger muscarinic toxin 7

Gene
N/A
Organism
Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds irreversibly and specifically to an allosteric site of the muscarinic acetylcholine M1 receptor (CHRM1).4 Publications

GO - Molecular functioni

  • receptor antagonist activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled acetylcholine receptor impairing toxin, G-protein coupled receptor impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Three-finger muscarinic toxin 71 Publication
Short name:
MT-7
Short name:
MT71 Publication
Alternative name(s):
Muscarinic m1-toxin 11 Publication
OrganismiDendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
Taxonomic identifieri8618 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeDendroaspis

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • other organism postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi55 – 551R → A: 105-fold decrease in affinity to M1 muscarinic acetylcholine receptors and change from irreversible to reversible binding. 1 Publication
Mutagenesisi59 – 591F → I: Decrease in affinity to M1 muscarinic acetylcholine receptors and change from irreversible to reversible binding. 1 Publication
Mutagenesisi86 – 861K → E: Decrease in affinity to M1 muscarinic acetylcholine receptors. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21213 PublicationsAdd
BLAST
Chaini22 – 8665Three-finger muscarinic toxin 73 PublicationsPRO_0000035481Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi24 ↔ 451 Publication
Disulfide bondi38 ↔ 631 Publication
Disulfide bondi67 ↔ 781 Publication
Disulfide bondi79 ↔ 841 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Interactioni

GO - Molecular functioni

  • receptor antagonist activity Source: UniProtKB

Structurei

Secondary structure

1
86
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi23 – 275Combined sources
Beta strandi29 – 313Combined sources
Beta strandi34 – 374Combined sources
Beta strandi44 – 5310Combined sources
Beta strandi56 – 6611Combined sources
Beta strandi75 – 795Combined sources
Turni82 – 854Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VLWX-ray1.39A/B22-86[»]
3FEVX-ray1.30A/B/C38-86[»]
3NEQX-ray1.25A/B22-69[»]
A/B78-86[»]
ProteinModelPortaliQ8QGR0.
SMRiQ8QGR0. Positions 22-85.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8QGR0.

Family & Domainsi

Sequence similaritiesi

Belongs to the snake three-finger toxin family. Short-chain subfamily. Aminergic toxin sub-subfamily.1 Publication

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006553.

Family and domain databases

InterProiIPR003571. Snake_3FTx.
IPR018354. Snake_toxin_con_site.
[Graphical view]
PROSITEiPS00272. SNAKE_TOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8QGR0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLLLTLVV VTIVCLDLGY TLTCVKSNSI WFPTSEDCPD GQNLCFKRWQ
60 70 80
YISPRMYDFT RGCAATCPKA EYRDVINCCG TDKCNK
Length:86
Mass (Da):9,771
Last modified:June 1, 2002 - v1
Checksum:i3AA85611258E5B9C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 502WQ → HW AA sequence (PubMed:8410188).Curated

Mass spectrometryi

Molecular mass is 7470.6 Da from positions 22 - 85. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241871 mRNA. Translation: AAM00185.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241871 mRNA. Translation: AAM00185.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VLWX-ray1.39A/B22-86[»]
3FEVX-ray1.30A/B/C38-86[»]
3NEQX-ray1.25A/B22-69[»]
A/B78-86[»]
ProteinModelPortaliQ8QGR0.
SMRiQ8QGR0. Positions 22-85.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG006553.

Miscellaneous databases

EvolutionaryTraceiQ8QGR0.

Family and domain databases

InterProiIPR003571. Snake_3FTx.
IPR018354. Snake_toxin_con_site.
[Graphical view]
PROSITEiPS00272. SNAKE_TOXIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Site-directed mutagenesis of m1-toxin1: two amino acids responsible for stable toxin binding to m1 muscarinic receptors."
    Krajewski J.L., Dickerson I.M., Potter L.T.
    Mol. Pharmacol. 60:725-731(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MASS SPECTROMETRY, MUTAGENESIS OF PHE-59 AND LYS-86.
    Tissue: Venom gland.
  2. "Purification and properties of m1-toxin, a specific antagonist of m1 muscarinic receptors."
    Max S.I., Liang J.-S., Potter L.T.
    J. Neurosci. 13:4293-4300(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-85, SUBCELLULAR LOCATION.
    Tissue: Venom.
  3. "Recombinant expression of a selective blocker of m1 muscarinic receptors."
    Naesman J., Jolkkonen M., Ammoun S., Karlsson E., Aakerman K.E.O.
    Biochem. Biophys. Res. Commun. 271:435-439(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-86, FUNCTION.
    Tissue: Venom.
  4. "M1-toxin isotoxins from the green mamba (Dendroaspis angusticeps) that selectively block m1 muscarinic receptors."
    Carsi J.M., Potter L.T.
    Toxicon 38:187-198(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-86.
    Tissue: Venom.
  5. "Chemical synthesis of MT1 and MT7 muscarinic toxins: critical role of Arg-34 in their interaction with M1 muscarinic receptor."
    Mourier G., Dutertre S., Fruchart-Gaillard C., Menez A., Servent D.
    Mol. Pharmacol. 63:26-35(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SYNTHESIS OF 22-86, MUTAGENESIS OF ARG-55, FUNCTION.
  6. "Adrenoceptor activity of muscarinic toxins identified from mamba venoms."
    Naereoja K., Kukkonen J.P., Rondinelli S., Toivola D.M., Meriluoto J., Naesman J.
    Br. J. Pharmacol. 164:538-550(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Polypharmacology profiles and phylogenetic analysis of three-finger toxins from mamba venom: case of aminergic toxins."
    Blanchet G., Collet G., Mourier G., Gilles N., Fruchart-Gaillard C., Marcon E., Servent D.
    Biochimie 103:109-117(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: SYNTHESIS OF 22-86.
  8. "Different interactions between MT7 toxin and the human muscarinic M1 receptor in its free and N-methylscopolamine-occupied states."
    Fruchart-Gaillard C., Mourier G., Marquer C., Stura E., Birdsall N.J., Servent D.
    Mol. Pharmacol. 74:1554-1563(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.39 ANGSTROMS) OF 22-86, DISULFIDE BONDS.
  9. "Engineering of three-finger fold toxins creates ligands with original pharmacological profiles for muscarinic and adrenergic receptors."
    Fruchart-Gaillard C., Mourier G., Blanchet G., Vera L., Gilles N., Menez R., Marcon E., Stura E.A., Servent D.
    PLoS ONE 7:E39166-E39166(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.25 ANGSTROMS) OF 22-86.

Entry informationi

Entry namei3SIM7_DENAN
AccessioniPrimary (citable) accession number: Q8QGR0
Secondary accession number(s): P80970, Q9PRZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: June 1, 2002
Last modified: July 6, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Does not show interaction with adrenergic receptors (ADRA1A, ADRA1B, ADRA1D, ADRA2A, ADRA2B, ADRA2C, ADRB1, ADRB2), dopaminergic receptors (DRD1, DRD2, DRD3, DRD4, DRD5), histaminic receptors (HRH1, HRH3, HRH4) and serotoninergic receptors (HTR1A, HTR2A, HTR2B, HTR2C, HTR5A, HTR6, HTR7) (PubMed:21557730, PubMed:24793485). Does not show interaction with muscarinic receptors (CHRM2, CHRM3, CHRM4, CHRM5) (PubMed:12488533, PubMed:24793485).3 Publications

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.