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Protein

Muscarinic toxin 7

Gene
N/A
Organism
Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds irreversibly and specifically to an allosteric site of the muscarinic acetylcholine M1 receptor (CHRM1).4 Publications

Miscellaneous

Does not show interaction with adrenergic receptors (ADRA1A, ADRA1B, ADRA1D, ADRA2A, ADRA2B, ADRA2C, ADRB1, ADRB2), dopaminergic receptors (DRD1, DRD2, DRD3, DRD4, DRD5), histaminic receptors (HRH1, HRH3, HRH4) and serotoninergic receptors (HTR1A, HTR2A, HTR2B, HTR2C, HTR5A, HTR6, HTR7) (PubMed:21557730, PubMed:24793485). Does not show interaction with muscarinic receptors (CHRM2, CHRM3, CHRM4, CHRM5) (PubMed:12488533, PubMed:24793485).3 Publications

GO - Molecular functioni

  • receptor antagonist activity Source: UniProtKB
  • toxin activity Source: UniProtKB-KW

Keywordsi

Molecular functionG-protein coupled acetylcholine receptor impairing toxin, G-protein coupled receptor impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic toxin 71 Publication
Short name:
MT-7
Short name:
MT71 Publication
Alternative name(s):
Muscarinic m1-toxin 11 Publication
OrganismiDendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
Taxonomic identifieri8618 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeDendroaspis

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi55R → A: 105-fold decrease in affinity to M1 muscarinic acetylcholine receptors and change from irreversible to reversible binding. 1 Publication1
Mutagenesisi59F → I: Decrease in affinity to M1 muscarinic acetylcholine receptors and change from irreversible to reversible binding. 1 Publication1
Mutagenesisi86K → E: Decrease in affinity to M1 muscarinic acetylcholine receptors. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 213 PublicationsAdd BLAST21
ChainiPRO_000003548122 – 86Muscarinic toxin 73 PublicationsAdd BLAST65

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 451 Publication
Disulfide bondi38 ↔ 631 Publication
Disulfide bondi67 ↔ 781 Publication
Disulfide bondi79 ↔ 841 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Interactioni

GO - Molecular functioni

  • receptor antagonist activity Source: UniProtKB

Structurei

Secondary structure

186
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 27Combined sources5
Beta strandi29 – 31Combined sources3
Beta strandi34 – 37Combined sources4
Beta strandi44 – 53Combined sources10
Beta strandi56 – 66Combined sources11
Beta strandi75 – 79Combined sources5
Turni82 – 85Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VLWX-ray1.39A/B22-86[»]
3FEVX-ray1.30A/B/C38-86[»]
3NEQX-ray1.25A/B22-69[»]
A/B78-86[»]
ProteinModelPortaliQ8QGR0
SMRiQ8QGR0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8QGR0

Family & Domainsi

Sequence similaritiesi

Belongs to the snake three-finger toxin family. Short-chain subfamily. Aminergic toxin sub-subfamily.1 Publication

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006553

Family and domain databases

CDDicd00206 snake_toxin, 1 hit
InterProiView protein in InterPro
IPR003571 Snake_3FTx
IPR018354 Snake_toxin_con_site
IPR035076 Toxin/TOLIP
PfamiView protein in Pfam
PF00087 Toxin_TOLIP, 1 hit
PROSITEiView protein in PROSITE
PS00272 SNAKE_TOXIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8QGR0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLLLTLVV VTIVCLDLGY TLTCVKSNSI WFPTSEDCPD GQNLCFKRWQ
60 70 80
YISPRMYDFT RGCAATCPKA EYRDVINCCG TDKCNK
Length:86
Mass (Da):9,771
Last modified:June 1, 2002 - v1
Checksum:i3AA85611258E5B9C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49 – 50WQ → HW AA sequence (PubMed:8410188).Curated2

Mass spectrometryi

Molecular mass is 7470.6 Da from positions 22 - 85. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241871 mRNA Translation: AAM00185.1

Similar proteinsi

Entry informationi

Entry namei3SIM7_DENAN
AccessioniPrimary (citable) accession number: Q8QGR0
Secondary accession number(s): P80970, Q9PRZ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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