Q8QG92 (DCT1B_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dapper 1-B
Short name=xDpr

Alternative name(s):
Dapper1a
Short name=XDpr1a

Gene names

Name:

dact1-b

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	824 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. Binds to dvl2 to regulate the degradation of beta-catenin (ctnnb1-A and possibly ctnnb1-B), thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Seems to promote beta-catenin degradation if not phosphorylated and to block beta-catenin degradation if phosphorylated by CaMK1D. Involved in regulation of catenin delta/ctnnd1 protein level. May also bind to and directly stimulate the activity of tcf7l1-A. Also regulates the activation by dvl2 of jnk, a component of ctnnb1/beta-catenin-independent frizzled signaling. Required for notochord and head formation. Ref.1 Ref.3 Ref.4 Ref.5
Subunit structure	Interacts with dbf4 and tcf7l1-A By similarity. Interacts with dvl2/dsh; the interaction is required for dact1-b phosphorylation by CaMK1D and seems to become disrupted by the phosphorylation. Ref.1
Subcellular location	Cytoplasm. Nucleus Ref.1.
Tissue specificity	Expressed both in the dorsal lip in early gastrula and throughout the posterior presumptive ectoderm in early neurula. Expressed in the dorsal neural folds at the tailbud stage and highly expressed in the tadpole head, including the brain, retina and cartilaginous branchial arch derivatives. Ref.1
Developmental stage	Expressed both maternally and zygotically.
Domain	The C-terminal PDZ-binding motif may mediate interaction with the PDZ domains of DSH (Dishevelled) family proteins.
Post-translational modification	Phosphorylated by CaMK1D; the phosphorylation requires binding to dvl2/dsh. Ref.5
Miscellaneous	Named 'dapper', an antonym of dishevelled, because it acts as an antagonist of dvl2/dsh.
Sequence similarities	Belongs to the dapper family.

Ontologies

Keywords
Biological process	Neurogenesis Wnt signaling pathway
Cellular component	Cytoplasm Nucleus
Domain	Coiled coil
Molecular function	Developmental protein
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of JNK cascade Inferred from direct assay Ref.1. Source: UniProtKB negative regulation of Wnt receptor signaling pathway Inferred from direct assay Ref.1. Source: UniProtKB nervous system development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
dvl2	P51142	2	EBI-6257549,EBI-6257503

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 824	824	Dapper 1-B		PRO_0000191356
Regions
	Region	2 – 343	342	Interaction with tcf7l1-A By similarity
	Coiled coil	84 – 139	56	Potential
	Motif	821 – 824	4	PDZ-binding
Experimental info
	Mutagenesis	821 – 824	4	Missing: Abrogates binding to dvl2. Ref.1
	Mutagenesis	822	1	T → N: Abrogates binding to dvl2 and abolishes phosphorylation by CaMK1D. Ref.1 Ref.5
	Sequence conflict	228	1	E → G in AAH77380. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8QG92 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F2172CD999DF7814	FASTA	824	90,958
	10 20 30 40 50 60 MKPIPAAPEP LGQHQDSPRR KDKGEAESER QRTRERLEAT LAGLAELGHL RHRQEVLIKS 70 80 90 100 110 120 VLSPGTRTHG DAAARTGDNP RSLEEKFLED NILLLKKQLN CLRKRDAGLL SQLHELDKQI 130 140 150 160 170 180 NDLRIDVEKT EEHLETDSRP SSGFYELSDG TSGSLSNSSN SVFSECLSSC HSSTCFCNPL 190 200 210 220 230 240 ETSLNLTDGQ AKSADDFLEW LDYRESQHET GTVRRSFSAP HSNSVDIEAD VHPKYQCDLV 250 260 270 280 290 300 SKNGNDIYRY PSPLHAVAVQ SPMFLLSVMG NIKAEEPEEG IDHNDNDDCI VPELDHLKDE 310 320 330 340 350 360 DSFLHQSSLC SLPLSSAKKM DGYILSIIQK KAHPVRTNKP RTSVNADPGK GILRHGSMCV 370 380 390 400 410 420 KQTGGVSQSN AVNLKNSKQT CLHSTGMIAV DNSTYPSLKQ CSKESLSEQL ESKRMPSIST 430 440 450 460 470 480 YPSCNVNELQ SQNNSRNTVK SVCQGLARGS VAMTSNVQKE NVTPNALANL SNTSSSVCNV 490 500 510 520 530 540 TPGESMQNSP LLPQEIKVVP PVKRVSPQNT LLSYHASSSF DERPPLDFKS EGSSSQSLDE 550 560 570 580 590 600 GLLVNAHYIP AQQQGVKLHK HTKYVKIVKS STLKHRANVQ YVAENGSQTL KEKSKVVGKK 610 620 630 640 650 660 CRFPDDLDTN KKVKKSTLRV KKTAHPHFEP AVVGRNPVAV RSGSKSHGHS KDVVLAKPKH 670 680 690 700 710 720 KRGDYRRWKS SAEISYEEAL RRARRRAQGE MVGVYAQVPF PYSSPYAYIA SDSEYSAECE 730 740 750 760 770 780 SLFHSTVVDT SEDEQSNYTT NCFGDSESSL SEVEFVGEST TSSDTDESGG LIWSQFVQTL 790 800 810 820 PMQATATAEL QTTAKAFVKI KASHNLKKKI LRFRSGSLKL MTTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation." Cheyette B.N.R., Waxman J.S., Miller J.R., Takemaru K., Sheldahl L.C., Khlebtsova N., Fox E.P., Earnest T.N., Moon R.T. Dev. Cell 2:449-461(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH DVL2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF THR-822 AND 821-MET--VAL-824. Tissue: Oocyte.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[3]	"The involvement of Frodo in TCF-dependent signaling and neural tissue development." Hikasa H., Sokol S.Y. Development 131:4725-4734(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals." Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D. Dev. Cell 11:683-695(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Dpr Acts as a molecular switch, inhibiting Wnt signaling when unphosphorylated, but promoting Wnt signaling when phosphorylated by casein kinase Idelta/epsilon." Teran E., Branscomb A.D., Seeling J.M. PLoS ONE 4:E5522-E5522(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF THR-822.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF488776 mRNA. Translation: AAM12548.1. BC077380 mRNA. Translation: AAH77380.1.
RefSeq	NP_001083910.1. NM_001090441.1.
UniGene	Xl.7602.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8QG92. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	399186.
KEGG	xla:399186.
Organism-specific databases
CTD	399186.
Xenbase	XB-GENE-478643. dact1.
Phylogenomic databases
HOVERGEN	HBG051286.
Family and domain databases
InterPro	IPR024843. Dapper. IPR024850. Dapper_xenopus. [Graphical view]
PANTHER	PTHR15919. PTHR15919. 1 hit. PTHR15919:SF4. PTHR15919:SF4. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DCT1B_XENLA

Accession

Primary (citable) accession number: Q8QG92
Secondary accession number(s): Q6DDX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	June 1, 2002
Last modified:	April 3, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program