Q8QG92 (DCT1B_XENLA) Reviewed, UniProtKB/Swiss-Prot
Last modified April 3, 2013. Version 46. History...
Names and origin
|Protein names||Recommended name:|
|Organism||Xenopus laevis (African clawed frog)|
|Taxonomic identifier||8355 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus|
|Sequence length||824 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. Binds to dvl2 to regulate the degradation of beta-catenin (ctnnb1-A and possibly ctnnb1-B), thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Seems to promote beta-catenin degradation if not phosphorylated and to block beta-catenin degradation if phosphorylated by CaMK1D. Involved in regulation of catenin delta/ctnnd1 protein level. May also bind to and directly stimulate the activity of tcf7l1-A. Also regulates the activation by dvl2 of jnk, a component of ctnnb1/beta-catenin-independent frizzled signaling. Required for notochord and head formation. Ref.1 Ref.3 Ref.4 Ref.5
Interacts with dbf4 and tcf7l1-A By similarity. Interacts with dvl2/dsh; the interaction is required for dact1-b phosphorylation by CaMK1D and seems to become disrupted by the phosphorylation. Ref.1
Expressed both in the dorsal lip in early gastrula and throughout the posterior presumptive ectoderm in early neurula. Expressed in the dorsal neural folds at the tailbud stage and highly expressed in the tadpole head, including the brain, retina and cartilaginous branchial arch derivatives. Ref.1
Expressed both maternally and zygotically.
The C-terminal PDZ-binding motif may mediate interaction with the PDZ domains of DSH (Dishevelled) family proteins.
Phosphorylated by CaMK1D; the phosphorylation requires binding to dvl2/dsh. Ref.5
Named 'dapper', an antonym of dishevelled, because it acts as an antagonist of dvl2/dsh.
Belongs to the dapper family.
Wnt signaling pathway
|Molecular function||Developmental protein|
|Gene Ontology (GO)|
|Biological_process||Wnt receptor signaling pathway|
Inferred from electronic annotation. Source: UniProtKB-KWnegative regulation of JNK cascadenegative regulation of Wnt receptor signaling pathwaynervous system development
Inferred from electronic annotation. Source: UniProtKB-KW
Inferred from electronic annotation. Source: UniProtKB-SubCellnucleus
Inferred from electronic annotation. Source: UniProtKB-SubCell
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 824||824||Dapper 1-B||PRO_0000191356|
|Region||2 – 343||342||Interaction with tcf7l1-A By similarity|
|Coiled coil||84 – 139||56||Potential|
|Motif||821 – 824||4||PDZ-binding|
|Mutagenesis||821 – 824||4||Missing: Abrogates binding to dvl2. Ref.1|
|Mutagenesis||822||1||T → N: Abrogates binding to dvl2 and abolishes phosphorylation by CaMK1D. Ref.1 Ref.5|
|Sequence conflict||228||1||E → G in AAH77380. Ref.2|
|||"Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation."|
Cheyette B.N.R., Waxman J.S., Miller J.R., Takemaru K., Sheldahl L.C., Khlebtsova N., Fox E.P., Earnest T.N., Moon R.T.
Dev. Cell 2:449-461(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH DVL2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF THR-822 AND 821-MET--VAL-824.
|||NIH - Xenopus Gene Collection (XGC) project|
Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
|||"The involvement of Frodo in TCF-dependent signaling and neural tissue development."|
Hikasa H., Sokol S.Y.
Development 131:4725-4734(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
|||"Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals."|
Park J.I., Ji H., Jun S., Gu D., Hikasa H., Li L., Sokol S.Y., McCrea P.D.
Dev. Cell 11:683-695(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
|||"Dpr Acts as a molecular switch, inhibiting Wnt signaling when unphosphorylated, but promoting Wnt signaling when phosphorylated by casein kinase Idelta/epsilon."|
Teran E., Branscomb A.D., Seeling J.M.
PLoS ONE 4:E5522-E5522(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF THR-822.
|AF488776 mRNA. Translation: AAM12548.1.|
BC077380 mRNA. Translation: AAH77380.1.
|RefSeq||NP_001083910.1. NM_001090441.1. |
3D structure databases
Protein-protein interaction databases
|IntAct||Q8QG92. 2 interactions.|
Protocols and materials databases
Genome annotation databases
|Xenbase||XB-GENE-478643. dact1. |
Family and domain databases
|InterPro||IPR024843. Dapper. |
|PANTHER||PTHR15919. PTHR15919. 1 hit. |
PTHR15919:SF4. PTHR15919:SF4. 1 hit.
|Accession||Primary (citable) accession number: Q8QG92|
Secondary accession number(s): Q6DDX1
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families