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Protein

Argininosuccinate synthase

Gene

argG

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86CitrullineUniRule annotation1
Binding sitei91CitrullineUniRule annotation1
Binding sitei116ATP; via amide nitrogenUniRule annotation1
Binding sitei118AspartateUniRule annotation1
Binding sitei122AspartateUniRule annotation1
Binding sitei122CitrullineUniRule annotation1
Binding sitei123AspartateUniRule annotation1
Binding sitei126CitrullineUniRule annotation1
Binding sitei172CitrullineUniRule annotation1
Binding sitei181CitrullineUniRule annotation1
Binding sitei257CitrullineUniRule annotation1
Binding sitei269CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 16ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAZ192952:G1FZI-40-MONOMER
UniPathwayiUPA00068; UER00113

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:MM_0037
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001486781 – 394Argininosuccinate synthaseAdd BLAST394

Proteomic databases

PRIDEiQ8Q0U5

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi192952.MM_0037

Structurei

3D structure databases

ProteinModelPortaliQ8Q0U5
SMRiQ8Q0U5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00112 Archaea
COG0137 LUCA
HOGENOMiHOG000230093
KOiK01940
OMAiGVGRIDM
OrthoDBiPOG093Z02NI

Family and domain databases

CDDicd01999 Argininosuccinate_Synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
3.90.1260.10, 1 hit
HAMAPiMF_00005 Arg_succ_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001518 Arginosuc_synth
IPR018223 Arginosuc_synth_CS
IPR023434 Arginosuc_synth_type_1_subfam
IPR024074 AS_cat/multimer_dom_body
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR11587 PTHR11587, 1 hit
PfamiView protein in Pfam
PF00764 Arginosuc_synth, 1 hit
TIGRFAMsiTIGR00032 argG, 1 hit
PROSITEiView protein in PROSITE
PS00564 ARGININOSUCCIN_SYN_1, 1 hit
PS00565 ARGININOSUCCIN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8Q0U5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKKVALAYS GGLDTSVCIP ILKEKYGYDE VITISVDVGQ PEEEIRKADA
60 70 80 90 100
KAQKISNKHY TIDAKEEFVR DYIFPLIKAN GNYEGYVMGT SVARPLIAKK
110 120 130 140 150
VVEAAKKEGA IALAHGCTGK GNDQLRFEAV FRQTDMDVIA PMREMNLTRE
160 170 180 190 200
WEINYAKEHG IPVEATKSKP WSVDENIWSR SIEGGRLEDP SFVPPEEIFE
210 220 230 240 250
WTKSAEDAPN EPRIVDIDFE AGVPVAIDGE KLGGYALVKK MNEIAGENGV
260 270 280 290 300
GRTDMIEDRV LGLKARENYE HPAATVLLAA HADLEKLVLT RGELKFKKIV
310 320 330 340 350
EEQWSEMAYA GLVDDPLFAD LNAFIDKSQE RVTGTVKVKL YKGALTILAR
360 370 380 390
SSPNALYSED LVSFDSQTID QKDAEGFAKY HGFQARMYRK VVGK
Length:394
Mass (Da):43,888
Last modified:October 19, 2002 - v1
Checksum:i2770F2095D058B05
GO

Sequence cautioni

The sequence AAM29733 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA Translation: AAM29733.1 Different initiation.
RefSeqiWP_011031991.1, NC_003901.1

Genome annotation databases

EnsemblBacteriaiAAM29733; AAM29733; MM_0037
GeneIDi1478379
KEGGimma:MM_0037
PATRICifig|192952.21.peg.49

Similar proteinsi

Entry informationi

Entry nameiASSY_METMA
AccessioniPrimary (citable) accession number: Q8Q0U5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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