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Q8Q0J2 (PUR2_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:MM_0144
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length435 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 435435Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151512

Regions

Domain112 – 319208ATP-grasp
Nucleotide binding139 – 19658ATP By similarity

Sites

Metal binding2771Magnesium or manganese 1 By similarity
Metal binding2891Magnesium or manganese 1 By similarity
Metal binding2891Magnesium or manganese 2 By similarity
Metal binding2911Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8Q0J2 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: FC67D677ED0BC61C

FASTA43547,325
        10         20         30         40         50         60 
MKMKILLIGG GGREHAIAEG IKKSKHNPIL YALMAKKNPG IAALCEDFLL EKETEVEKVV 

        70         80         90        100        110        120 
EYAKARKIEM AFVGPEAPLA AGVADALWDA GIPVVGPKKA CAIIEFDKAW ARNFMKKYGI 

       130        140        150        160        170        180 
EGCPAYEVFT EEAPAHAFIE KLGDVAVKPS GLTGGKGVKV MGDQLPDIKA AREYTSELLE 

       190        200        210        220        230        240 
KGPVVIEERF IGEEFTLQAF VDGKNLAFFP AVQDHKRAYE GDIGPNTGGM GSYTDAGEIL 

       250        260        270        280        290        300 
PFMVPEDIEK AKKIMQHTVT ALREETGTGY QGILYGQFIL TSRGPKVVEF NARFGDPEAM 

       310        320        330        340        350        360 
NVIPLIETDF VEIMSAVTKG TLGDLPVKFS RKATVCKYAV PAGYPDNPKK DSEVIVGDIG 

       370        380        390        400        410        420 
EASVYYASVY EKEGKIYTTT SRAVAVVGCA ETIEAAEKVA QNALENIQGE LFFRRDIGTA 

       430 
SLIQKRIDHM KELRG 

« Hide

References

[1]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008384 Genomic DNA. Translation: AAM29840.1.
RefSeqNP_632168.1. NC_003901.1.

3D structure databases

ProteinModelPortalQ8Q0J2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING192952.MM_0144.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM29840; AAM29840; MM_0144.
GeneID1478486.
KEGGmma:MM_0144.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMADPEAMNV.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycMMAZ192952:GCK2-150-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_METMA
AccessionPrimary (citable) accession number: Q8Q0J2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways