Reviewed, UniProtKB/Swiss-Prot Q8Q0J2 (PUR2_METMA)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	MM_0144

Organism

Methanosarcina mazei (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

2209 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151512
Regions
	Domain	112 – 319	208	ATP-grasp
	Nucleotide binding	139 – 196	58	ATP By similarity
Sites
	Metal binding	277	1	Magnesium or manganese 1 By similarity
	Metal binding	289	1	Magnesium or manganese 1 By similarity
	Metal binding	289	1	Magnesium or manganese 2 By similarity
	Metal binding	291	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Q0J2-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: FC67D677ED0BC61C	FASTA	435	47,325
	10 20 30 40 50 60 MKMKILLIGG GGREHAIAEG IKKSKHNPIL YALMAKKNPG IAALCEDFLL EKETEVEKVV 70 80 90 100 110 120 EYAKARKIEM AFVGPEAPLA AGVADALWDA GIPVVGPKKA CAIIEFDKAW ARNFMKKYGI 130 140 150 160 170 180 EGCPAYEVFT EEAPAHAFIE KLGDVAVKPS GLTGGKGVKV MGDQLPDIKA AREYTSELLE 190 200 210 220 230 240 KGPVVIEERF IGEEFTLQAF VDGKNLAFFP AVQDHKRAYE GDIGPNTGGM GSYTDAGEIL 250 260 270 280 290 300 PFMVPEDIEK AKKIMQHTVT ALREETGTGY QGILYGQFIL TSRGPKVVEF NARFGDPEAM 310 320 330 340 350 360 NVIPLIETDF VEIMSAVTKG TLGDLPVKFS RKATVCKYAV PAGYPDNPKK DSEVIVGDIG 370 380 390 400 410 420 EASVYYASVY EKEGKIYTTT SRAVAVVGCA ETIEAAEKVA QNALENIQGE LFFRRDIGTA 430 SLIQKRIDHM KELRG

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008384 Genomic DNA. Translation: AAM29840.1.
RefSeq	NP_632168.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Genome annotation databases
GeneID	1478486.
GenomeReviews	Gene locus MM_0144 in contig AE008384_GR.
KEGG	mma:MM_0144.
NMPDR	fig|192952.1.peg.144.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8Q0J2.
OMA	IEYNCRF.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM0144-MON.
BRENDA	6.3.4.13. 261165.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_METMA

Accession

Primary (citable) accession number: Q8Q0J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)