SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8Q054

- SYL_METMA

UniProt

Q8Q054 - SYL_METMA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Leucine--tRNA ligase
Gene
leuS, MM_0283
Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei635 – 6351ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-297-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leuS
Ordered Locus Names:MM_0283
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000000595: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 966966Leucine--tRNA ligaseUniRule annotation
PRO_0000152133Add
BLAST

Proteomic databases

PRIDEiQ8Q054.

Interactioni

Protein-protein interaction databases

STRINGi192952.MM_0283.

Structurei

3D structure databases

ProteinModelPortaliQ8Q054.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionUniRule annotation
Add
BLAST
Motifi632 – 6365"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiHAPFDYL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8Q054-1 [UniParc]FASTAAdd to Basket

« Hide

MEQDYKPHEI ENKWQKKWNE SRIFQAEPDK REKFFITIPY PYLNGNLHAG    50
HTRTFTIGDV VARHKRMLGY NVLYPMGFHV TGTPIVGLAE LIASRDPQTM 100
DVYERLHGIP GDILPALDTP EKIVDYFKVE AEKAMRMIGY SIDWRRKFTT 150
TDPTYKKFIE WQYTRLEEKD LIVKGSHPVK WCPNDNNPVE DHDILHGEEA 200
TIVEYTLIKF RYRDLVLPCA TLRPETTYGV TNLWVNPNVD YVKARVEKDG 250
NVEFWVVSRD AFRKLTFTDR TVEYVEDMPA KSIIGIKLTN PVTGDEVISL 300
PASFVKPENG SGIVMSVPAH APFDYLALRD LYDADLSEYG ITEDLRKIEL 350
ISLIQVPEFG EFPAKEIVES MGISDQKDPK AEEATKIVYR REFHGGVLKE 400
LTGKYKGYPV SKIKDILTRD FLASNAGETF YEFSEPVVCR CGTPCVVNMV 450
KGQWFLNYSN PDWKAKVYKC LGQMRVIPTE YRVEFENKID WLKDKACARR 500
KGLGTRLPFD KEWLIESLGD STIYMSYYII ARFIEKGELA LEHLTLSFFD 550
YVLLGKGDSA AVSAETGLKP ELIEEIRSHF NYWYPVDLRS SGKDLVPNHL 600
LFFLFHHVAL FEEEKWPKAL AVNGFVSLEG QKMSKSKGPI LTLESAVSSY 650
GADITRMYIL STAEQTQDAD WQKAGIDSAR RQVDRFYAFA KDVIESGKRG 700
TLSAELKQID RWMLSRMQNY IKETNAALDS IQTREAIQNS FFLLINDVRW 750
YQRRGGEALL YYVLDNWVRL MAPFTPHLCE EVWEAMGHED PVSLAQYPLY 800
NEDLIDNGAE LSEEAVKSTL NDIEEIIRVT KMTPQKVYLY TAPAWKAEAI 850
RCACGLQVEA PLEVGTLIKT LMAKPELKRF GKEIPKFVQK IVPEFKSGGA 900
ERYETFAYLG LDEKDLLKES ASFLEKEIGC PVEIQSADSP EYDPQKKSRF 950
AEPLRPAIYI EEKKEE 966
Length:966
Mass (Da):110,957
Last modified:March 1, 2005 - v2
Checksum:i561D0FDC574A0DC3
GO

Sequence cautioni

The sequence AAM29979.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008384 Genomic DNA. Translation: AAM29979.1. Different initiation.
RefSeqiNP_632307.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM29979; AAM29979; MM_0283.
GeneIDi1478625.
KEGGimma:MM_0283.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008384 Genomic DNA. Translation: AAM29979.1 . Different initiation.
RefSeqi NP_632307.1. NC_003901.1.

3D structure databases

ProteinModelPortali Q8Q054.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 192952.MM_0283.

Proteomic databases

PRIDEi Q8Q054.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM29979 ; AAM29979 ; MM_0283 .
GeneIDi 1478625.
KEGGi mma:MM_0283.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000216620.
KOi K01869.
OMAi HAPFDYL.

Enzyme and pathway databases

BioCyci MMAZ192952:GCK2-297-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_A. Leu_tRNA_synth_A.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
    Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R.
    , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Entry informationi

Entry nameiSYL_METMA
AccessioniPrimary (citable) accession number: Q8Q054
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi