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Q8Q054 (SYL_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MM_0283
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length966 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAM29979.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 966966Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152133

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif632 – 6365"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6351ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8Q054 [UniParc].

Last modified March 1, 2005. Version 2.
Checksum: 561D0FDC574A0DC3

FASTA966110,957
        10         20         30         40         50         60 
MEQDYKPHEI ENKWQKKWNE SRIFQAEPDK REKFFITIPY PYLNGNLHAG HTRTFTIGDV 

        70         80         90        100        110        120 
VARHKRMLGY NVLYPMGFHV TGTPIVGLAE LIASRDPQTM DVYERLHGIP GDILPALDTP 

       130        140        150        160        170        180 
EKIVDYFKVE AEKAMRMIGY SIDWRRKFTT TDPTYKKFIE WQYTRLEEKD LIVKGSHPVK 

       190        200        210        220        230        240 
WCPNDNNPVE DHDILHGEEA TIVEYTLIKF RYRDLVLPCA TLRPETTYGV TNLWVNPNVD 

       250        260        270        280        290        300 
YVKARVEKDG NVEFWVVSRD AFRKLTFTDR TVEYVEDMPA KSIIGIKLTN PVTGDEVISL 

       310        320        330        340        350        360 
PASFVKPENG SGIVMSVPAH APFDYLALRD LYDADLSEYG ITEDLRKIEL ISLIQVPEFG 

       370        380        390        400        410        420 
EFPAKEIVES MGISDQKDPK AEEATKIVYR REFHGGVLKE LTGKYKGYPV SKIKDILTRD 

       430        440        450        460        470        480 
FLASNAGETF YEFSEPVVCR CGTPCVVNMV KGQWFLNYSN PDWKAKVYKC LGQMRVIPTE 

       490        500        510        520        530        540 
YRVEFENKID WLKDKACARR KGLGTRLPFD KEWLIESLGD STIYMSYYII ARFIEKGELA 

       550        560        570        580        590        600 
LEHLTLSFFD YVLLGKGDSA AVSAETGLKP ELIEEIRSHF NYWYPVDLRS SGKDLVPNHL 

       610        620        630        640        650        660 
LFFLFHHVAL FEEEKWPKAL AVNGFVSLEG QKMSKSKGPI LTLESAVSSY GADITRMYIL 

       670        680        690        700        710        720 
STAEQTQDAD WQKAGIDSAR RQVDRFYAFA KDVIESGKRG TLSAELKQID RWMLSRMQNY 

       730        740        750        760        770        780 
IKETNAALDS IQTREAIQNS FFLLINDVRW YQRRGGEALL YYVLDNWVRL MAPFTPHLCE 

       790        800        810        820        830        840 
EVWEAMGHED PVSLAQYPLY NEDLIDNGAE LSEEAVKSTL NDIEEIIRVT KMTPQKVYLY 

       850        860        870        880        890        900 
TAPAWKAEAI RCACGLQVEA PLEVGTLIKT LMAKPELKRF GKEIPKFVQK IVPEFKSGGA 

       910        920        930        940        950        960 
ERYETFAYLG LDEKDLLKES ASFLEKEIGC PVEIQSADSP EYDPQKKSRF AEPLRPAIYI 


EEKKEE 

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References

[1]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008384 Genomic DNA. Translation: AAM29979.1. Different initiation.
RefSeqNP_632307.1. NC_003901.1.

3D structure databases

ProteinModelPortalQ8Q054.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING192952.MM_0283.

Proteomic databases

PRIDEQ8Q054.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM29979; AAM29979; MM_0283.
GeneID1478625.
KEGGmma:MM_0283.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAHAPFDYL.
ProtClustDBPRK12300.

Enzyme and pathway databases

BioCycMMAZ192952:GCK2-297-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_METMA
AccessionPrimary (citable) accession number: Q8Q054
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: April 16, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries