Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable deoxyhypusine synthase 2

Gene

dys2

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.By similarity

Catalytic activityi

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.

Cofactori

NAD+By similarity

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei292 – 2921NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-301-MONOMER.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable deoxyhypusine synthase 2 (EC:2.5.1.46)
Short name:
DHS 2
Gene namesi
Name:dys2
Ordered Locus Names:MM_0287
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Probable deoxyhypusine synthase 2PRO_0000134498Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi192952.MM_0287.

Structurei

3D structure databases

ProteinModelPortaliQ8Q051.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the deoxyhypusine synthase family.Curated

Phylogenomic databases

eggNOGiarCOG04142. Archaea.
COG1899. LUCA.
HOGENOMiHOG000228839.
KOiK00809.
OMAiSGCTFDE.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
HAMAPiMF_00153. DHS.
InterProiIPR022899. Deoxyhypus_synthase_arc.
IPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8Q051-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHHNVFTNTP TIPIDVKDRS VSELMDGMLR TGFQGRKLAE SVQAWSNMLK
60 70 80 90 100
EKDTTVLMGL SGAMVPAGMR RVISYLIRER MIDCLVSTGA NLFHDSHEAL
110 120 130 140 150
GRKHYVGSHL ANDEKLFEHG VDRIYDVFAV EEEFRNADNL IADFAEEIGE
160 170 180 190 200
ISCSSREFMY LLGKELVRRG AAEDSIVVSA YRHNVPIFVP ALSDSSIGIG
210 220 230 240 250
LTIARRRGLK LEIDQIKDVD EITQIVEKSG HTGVVYVGGG VPKNFIQQTE
260 270 280 290 300
VIASILGMDV PGHEYAIQYT SDSPHWGGLS GCTFDEAVSW GKVAAQAKKV
310 320 330 340
QVFVDATIAL PIVAHALHEK TRGVKRTAPV FSWDGPEGLE IAYNE
Length:345
Mass (Da):38,042
Last modified:October 10, 2002 - v1
Checksum:i8772506516B76A9D
GO

Sequence cautioni

The sequence AAM29983.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM29983.1. Different initiation.
RefSeqiWP_015410997.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM29983; AAM29983; MM_0287.
GeneIDi24882353.
KEGGimma:MM_0287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM29983.1. Different initiation.
RefSeqiWP_015410997.1. NC_003901.1.

3D structure databases

ProteinModelPortaliQ8Q051.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_0287.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM29983; AAM29983; MM_0287.
GeneIDi24882353.
KEGGimma:MM_0287.

Phylogenomic databases

eggNOGiarCOG04142. Archaea.
COG1899. LUCA.
HOGENOMiHOG000228839.
KOiK00809.
OMAiSGCTFDE.

Enzyme and pathway databases

UniPathwayiUPA00354.
BioCyciMMAZ192952:GCK2-301-MONOMER.

Family and domain databases

Gene3Di3.40.910.10. 1 hit.
HAMAPiMF_00153. DHS.
InterProiIPR022899. Deoxyhypus_synthase_arc.
IPR002773. Deoxyhypusine_synthase.
IPR029035. DHS-like_NAD/FAD-binding_dom.
[Graphical view]
PANTHERiPTHR11703. PTHR11703. 1 hit.
PfamiPF01916. DS. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
TIGRFAMsiTIGR00321. dhys. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
    Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R.
    , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Entry informationi

Entry nameiDHYS2_METMA
AccessioniPrimary (citable) accession number: Q8Q051
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: October 10, 2002
Last modified: November 11, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.