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Protein

Ribonuclease Z

Gene

rnz

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Zinc 1; catalyticUniRule annotation
Metal bindingi63 – 631Zinc 1; catalyticUniRule annotation
Active sitei65 – 651Proton acceptorUniRule annotation
Metal bindingi65 – 651Zinc 2; catalyticUniRule annotation
Metal bindingi66 – 661Zinc 2; catalyticUniRule annotation
Metal bindingi138 – 1381Zinc 1; catalyticUniRule annotation
Metal bindingi208 – 2081Zinc 1; catalyticUniRule annotation
Metal bindingi208 – 2081Zinc 2; catalyticUniRule annotation
Metal bindingi266 – 2661Zinc 2; catalyticUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-320-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease ZUniRule annotation (EC:3.1.26.11UniRule annotation)
Short name:
RNase ZUniRule annotation
Alternative name(s):
tRNA 3 endonucleaseUniRule annotation
tRNase ZUniRule annotation
Gene namesi
Name:rnzUniRule annotation
Ordered Locus Names:MM_0306
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Ribonuclease ZPRO_0000155926Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi192952.MM_0306.

Structurei

3D structure databases

ProteinModelPortaliQ8Q032.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase Z family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00501. Archaea.
COG1234. LUCA.
HOGENOMiHOG000272419.
KOiK00784.
OMAiSYAFCSD.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01818. RNase_Z_BN. 1 hit.
InterProiIPR001279. Metallo-B-lactamas.
IPR013471. RNase_Z/BN.
[Graphical view]
PfamiPF12706. Lactamase_B_2. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR02651. RNase_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8Q032-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRIIFLGTG GSLPTRNRNP SAVIVNLKGE LLLFDCGEGT QQQMMRAKTG
60 70 80 90 100
MMSLSSIFVS HFHADHFLGI PGLIQTMSFL GRKEPLTIYG PEGTKEFTEV
110 120 130 140 150
FKVLGYCNLK YEVRGVELSP GDIVEGKNYV VRALKTEHST PSLGYSLIEN
160 170 180 190 200
PRPGRFNREK AVELGVLPGP LFAKLQKGNP VEVNGKLVKP EEVVGAPRPG
210 220 230 240 250
RTVVYSGDTR PCEAVLEASR DADVLIHDGS FADEMAGWAE ESMHSTAGEV
260 270 280 290 300
ASLAKEAGVR QLVLTHISSR YTDDVGPILN DSRKVFENVI VAEDLMELEV

PYRPD
Length:305
Mass (Da):33,414
Last modified:October 1, 2002 - v1
Checksum:i32CF3C932C4BB1FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM30002.1.
RefSeqiWP_011032260.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM30002; AAM30002; MM_0306.
GeneIDi24882333.
KEGGimma:MM_0306.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM30002.1.
RefSeqiWP_011032260.1. NC_003901.1.

3D structure databases

ProteinModelPortaliQ8Q032.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_0306.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM30002; AAM30002; MM_0306.
GeneIDi24882333.
KEGGimma:MM_0306.

Phylogenomic databases

eggNOGiarCOG00501. Archaea.
COG1234. LUCA.
HOGENOMiHOG000272419.
KOiK00784.
OMAiSYAFCSD.

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-320-MONOMER.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01818. RNase_Z_BN. 1 hit.
InterProiIPR001279. Metallo-B-lactamas.
IPR013471. RNase_Z/BN.
[Graphical view]
PfamiPF12706. Lactamase_B_2. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR02651. RNase_Z. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNZ_METMA
AccessioniPrimary (citable) accession number: Q8Q032
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: October 1, 2002
Last modified: December 9, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.