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Protein

Bifunctional protein FolD

Gene

folD

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei196NADP; via amide nitrogenUniRule annotation1
Binding sitei237NADP; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi171 – 173NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:MM_0441
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002685851 – 287Bifunctional protein FolDAdd BLAST287

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi192952.MM_0441.

Structurei

3D structure databases

ProteinModelPortaliQ8PZQ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04538. Archaea.
COG0190. LUCA.
HOGENOMiHOG000218242.
KOiK01491.
OMAiGQPMALM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8PZQ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDESYESRI IDGKVLAQQV EEEVRSGVED LESNRGITPG LATILVGDDP
60 70 80 90 100
ASKMYVRLKH RACERVGIRA EDFLLPADST QEELLALIDS LNNNKDVHGI
110 120 130 140 150
LLQLPLPEHF SPQEAMEAIS PAKDADGFHP YNMGKLMIGD EGLVPCTPHG
160 170 180 190 200
VIRALEEYNV PVKGKNAVIV GHSNVVGKPM AAMLLNRNAS VSVCHIFTDD
210 220 230 240 250
LKKYTLGADI LVVAAGVKHL IKADMVKEGA VIFDVGITKE EDGVYGDVDF
260 270 280
ENVIKKASLI TPVPGGVGPL TVAMLMKHVL MCAEKSL
Length:287
Mass (Da):31,015
Last modified:October 1, 2002 - v1
Checksum:iD87F14DF01EC787F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM30137.1.
RefSeqiWP_011032394.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM30137; AAM30137; MM_0441.
GeneIDi24880745.
KEGGimma:MM_0441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM30137.1.
RefSeqiWP_011032394.1. NC_003901.1.

3D structure databases

ProteinModelPortaliQ8PZQ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_0441.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM30137; AAM30137; MM_0441.
GeneIDi24880745.
KEGGimma:MM_0441.

Phylogenomic databases

eggNOGiarCOG04538. Archaea.
COG0190. LUCA.
HOGENOMiHOG000218242.
KOiK01491.
OMAiGQPMALM.

Enzyme and pathway databases

UniPathwayiUPA00193.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOLD_METMA
AccessioniPrimary (citable) accession number: Q8PZQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.