ID   NADE_METMA              Reviewed;         256 AA.
AC   Q8PZP6;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 43.
DE   RecName: Full=NH(3)-dependent NAD(+) synthetase;
DE            EC=6.3.1.5;
GN   Name=nadE; OrderedLocusNames=MM_0446;
OS   Methanosarcina mazei (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales;
OC   Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=2209;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88;
RX   MEDLINE=22120827; PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene
RT   transfer between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
CC   -!- CATALYTIC ACTIVITY: ATP + deamido-NAD(+) + NH(3) = AMP +
CC       diphosphate + NAD(+).
CC   -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from
CC       deamido-NAD(+) (ammonia route): step 1/1.
CC   -!- SIMILARITY: Belongs to the NAD synthetase family.
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DR   EMBL; AE008384; AAM30142.1; -; Genomic_DNA.
DR   RefSeq; NP_632470.1; -.
DR   HSSP; P08164; 1IFX.
DR   GeneID; 1478788; -.
DR   GenomeReviews; AE008384_GR; MM_0446.
DR   KEGG; mma:MM_0446; -.
DR   NMPDR; fig|192952.1.peg.446; -.
DR   HOGENOM; Q8PZP6; -.
DR   OMA; Q8PZP6; CAINPIG.
DR   BioCyc; MMAZ192952:MM0446-MON; -.
DR   BRENDA; 6.3.1.5; 261165.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0008795; F:NAD+ synthase activity; IEA:EC.
DR   GO; GO:0009435; P:NAD biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00193; -; 1.
DR   InterPro; IPR003694; NAD_synthase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   Pfam; PF02540; NAD_synthase; 1.
DR   TIGRFAMs; TIGR00552; nadE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Ligase; NAD; Nucleotide-binding.
FT   CHAIN         1    256       NH(3)-dependent NAD(+) synthetase.
FT                                /FTId=PRO_0000152226.
FT   NP_BIND      29     36       ATP (By similarity).
FT   ACT_SITE     31     31       By similarity.
SQ   SEQUENCE   256 AA;  27786 MW;  679195AE15E1D9C9 CRC64;
     MDLENAQNRI IDFIRDETGR AGVKGVVVGI SGGIDSALTA TLAVKALGKD RVLGIHMPES
     SLTPAVDSED AEALADWLGI EYRTIDISGI ISAFMAAVPE SESADRLTKG NLKARTRMSL
     LYFHANRLNR MVIGTGNKTE ILLGYYTKYG DGGVDLEPIG GLYKTGVWEL SSRLGIPESL
     ITKKPSAGLW AGQTDEADLG ISYVKVDEVL KMIEDGVEPE VILDKTGISA DQLNSVTRRI
     ERNEHKRKAP PVPELY
//