Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei289UniRule annotation1
Active sitei300UniRule annotation1
Active sitei331UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:MM_0512
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094881 – 353tRNA-splicing endonucleaseAdd BLAST353

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi192952.MM_0512

Structurei

3D structure databases

ProteinModelPortaliQ8PZI1
SMRiQ8PZI1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01701 Archaea
COG1676 LUCA
HOGENOMiHOG000226561
KOiK01170
OMAiGADFRTY
OrthoDBiPOG093Z0DMC

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
HAMAPiMF_01834 EndA_long, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR036740 tRNA_intron_Endonuc_N_sf
IPR006676 tRNA_splic
IPR023516 tRNA_splic_arch_long
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 2 hits
PF02778 tRNA_int_endo_N, 2 hits
SUPFAMiSSF53032 SSF53032, 2 hits
SSF55267 SSF55267, 2 hits
TIGRFAMsiTIGR00324 endA, 2 hits

Sequencei

Sequence statusi: Complete.

Q8PZI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLLKTQLKG DRVLAGKEAV AELYKTGYFG RPKDDGLELS LVEAAYLQSR
60 70 80 90 100
GKLDIELEGK LLGFRAFFEQ ASLRQQNFEL KYIVYKDLKE RGYYVQPSAA
110 120 130 140 150
DFRVYPRGSH PGKSAAKIFV HVLSERQPLS VKLLQESVAS AENVHKQFIL
160 170 180 190 200
AVVDEESDLT FYEIKSASPK GEMPEPFPAV KADATFLEDR VIAWDAEASG
210 220 230 240 250
ALYSRGFYGK MLDPERLQLS LVESLYLFSR GVIVVRDRKG KIFSFDEFVE
260 270 280 290 300
KASEIEGSFL RKYSAYKALR DSGHVVKTGF KFGTHFRVYR KVESIEKIPH
310 320 330 340 350
SEYLVNVIPE DYEFRLPVMS GAVRLANSVR KRMLFAVEKG EEVEYLDIGR

VKM
Length:353
Mass (Da):40,191
Last modified:October 1, 2002 - v1
Checksum:i15D5DBAD60D3F7CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA Translation: AAM30208.1

Genome annotation databases

EnsemblBacteriaiAAM30208; AAM30208; MM_0512
KEGGimma:MM_0512
PATRICifig|192952.21.peg.610

Similar proteinsi

Entry informationi

Entry nameiENDA_METMA
AccessioniPrimary (citable) accession number: Q8PZI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health