Q8PZ91 (NTPA_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

MM_0603

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 184	184	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178276
Regions
	Region	8 – 13	6	Substrate binding By similarity
	Region	66 – 67	2	Substrate binding By similarity
Sites
	Metal binding	37	1	Magnesium or manganese By similarity
	Metal binding	66	1	Magnesium or manganese By similarity
	Binding site	145	1	Substrate By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	169	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PZ91 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 1E397C5D516BDF8B	FASTA	184	20,525
	10 20 30 40 50 60 MHKIVFVTGN KGKFAEVRDI LKNFGIEAIQ NKDGYPELQE DELEPIAANG AQYVANKLNM 70 80 90 100 110 120 PVMVDDSGIF INALNGFPGP YSRFVEDKLG NPKVLKLMEG EKDRSAYFKT VIGYCEPGQE 130 140 150 160 170 180 PLVFPGVVEG KIAYEERGTG GFGYDPIFEY NGMTFGELGD EEKNKVSHRR RAVDNFLEWF IGGA

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM30299.1.
RefSeq	NP_632627.1. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PZ91.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1478945.
GenomeReviews	Gene locus MM_0603 in contig AE008384_GR.
KEGG	mma:MM_0603.
NMPDR	fig|192952.1.peg.603.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG697237.
OMA	VEDSGFF.
PhylomeDB	Q8PZ91.
ProtClustDB	PRK14821.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM0603-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_METMA

Accession

Primary (citable) accession number: Q8PZ91

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program