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Q8PZ66 (MER_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5,10-methylenetetrahydromethanopterin reductase

EC=1.5.98.2
Alternative name(s):
Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase
Methylene-H(4)MPT reductase
Gene names
Name:mer
Ordered Locus Names:MM_0628
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible reduction of methylene-H4MPT to methyl-H4MPT By similarity. HAMAP-Rule MF_01091

Catalytic activity

5-methyltetrahydromethanopterin + oxidized coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420. HAMAP-Rule MF_01091

Pathway

One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2. HAMAP-Rule MF_01091

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01091.

Sequence similarities

Belongs to the mer family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3283285,10-methylenetetrahydromethanopterin reductase HAMAP-Rule MF_01091
PRO_0000084809

Sequences

Sequence LengthMass (Da)Tools
Q8PZ66 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: B5120CB00E004542

FASTA32834,812
        10         20         30         40         50         60 
MKFGIEFVPA DPALKIAYYA KLSEQQGFDY VWITDHYNNR DVYSTLTVLA LNTNSIKIGS 

        70         80         90        100        110        120 
GVTNSYTRNP AITASSIASI AEISGGRAVL GLGPGDKATF DAMGIAWEKP LATTKEAIQA 

       130        140        150        160        170        180 
IRDFIDGKKV SMDGEMVKFA GAKLAFKAGK IPIYMGAQGP KMLELAGEVA DGVLINASHP 

       190        200        210        220        230        240 
KDFEVAVEQI RKGAEKVGRD PSEVDVTAYA CFSIDKDPVK AVNAAKVVVA FIVAGSPDLV 

       250        260        270        280        290        300 
LERHGISVEA KKQIGDAIAK GDFGSLMGGL VTPQMIEAFS ICGTPEDCMK RIKDLEAIGV 

       310        320 
TQIVAGSPIG PDKEKAIKLI GKEIIAKM 

« Hide

References

[1]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008384 Genomic DNA. Translation: AAM30324.1.
RefSeqNP_632652.1. NC_003901.1.

3D structure databases

ProteinModelPortalQ8PZ66.
SMRQ8PZ66. Positions 1-327.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING192952.MM_0628.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM30324; AAM30324; MM_0628.
GeneID1478970.
KEGGmma:MM_0628.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2141.
HOGENOMHOG000223871.
KOK00320.
OMAIGPNKEK.

Enzyme and pathway databases

BioCycMMAZ192952:GCK2-644-MONOMER.
UniPathwayUPA00640; UER00697.

Family and domain databases

Gene3D3.20.20.30. 1 hit.
HAMAPMF_01091. F420_mer.
InterProIPR011251. Luciferase-like_dom.
IPR019946. MeH4methanopterin_reductase.
[Graphical view]
PfamPF00296. Bac_luciferase. 1 hit.
[Graphical view]
SUPFAMSSF51679. SSF51679. 1 hit.
TIGRFAMsTIGR03555. F420_mer. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMER_METMA
AccessionPrimary (citable) accession number: Q8PZ66
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: October 1, 2002
Last modified: May 14, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways