Q8PZ66 (MER_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5,10-methylenetetrahydromethanopterin reductase
EC=1.5.99.11

Alternative name(s):
Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase
Methylene-H(4)MPT reductase

Gene names

Name:	mer
Ordered Locus Names:	MM_0628

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina mazei

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reduction of methylene-H4MPT to methyl-H4MPT By similarity. HAMAP-Rule MF_01091
Catalytic activity	5-methyltetrahydromethanopterin + coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420. HAMAP-Rule MF_01091
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2. HAMAP-Rule MF_01091
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mer family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	methanogenesis, from carbon dioxide Inferred from electronic annotation. Source: UniProtKB-UniPathway one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	5,10-methylenetetrahydromethanopterin reductase HAMAP-Rule MF_01091		PRO_0000084809

Sequences

	Sequence		Length	Mass (Da)
	Q8PZ66 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: B5120CB00E004542	FASTA	328	34,812
	10 20 30 40 50 60 MKFGIEFVPA DPALKIAYYA KLSEQQGFDY VWITDHYNNR DVYSTLTVLA LNTNSIKIGS 70 80 90 100 110 120 GVTNSYTRNP AITASSIASI AEISGGRAVL GLGPGDKATF DAMGIAWEKP LATTKEAIQA 130 140 150 160 170 180 IRDFIDGKKV SMDGEMVKFA GAKLAFKAGK IPIYMGAQGP KMLELAGEVA DGVLINASHP 190 200 210 220 230 240 KDFEVAVEQI RKGAEKVGRD PSEVDVTAYA CFSIDKDPVK AVNAAKVVVA FIVAGSPDLV 250 260 270 280 290 300 LERHGISVEA KKQIGDAIAK GDFGSLMGGL VTPQMIEAFS ICGTPEDCMK RIKDLEAIGV 310 320 TQIVAGSPIG PDKEKAIKLI GKEIIAKM

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM30324.1.
RefSeq	NP_632652.1. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PZ66.
SMR	Q8PZ66. Positions 1-327.
ModBase	Search...
Protein-protein interaction databases
STRING	192952.MM_0628.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM30324; AAM30324; MM_0628.
GeneID	1478970.
KEGG	mma:MM_0628.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2141.
HOGENOM	HOG000223871.
KO	K00320.
OMA	PALKIAY.
ProtClustDB	PRK02271.
Enzyme and pathway databases
UniPathway	UPA00640; UER00697.
Family and domain databases
Gene3D	3.20.20.30. 1 hit.
HAMAP	MF_01091. F420_mer.
InterPro	IPR011251. Luciferase-like_dom. IPR019946. MeH4methanopterin_reductase. [Graphical view]
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
SUPFAM	SSF51679. Luciferase_like. 1 hit.
TIGRFAMs	TIGR03555. F420_mer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MER_METMA

Accession

Primary (citable) accession number: Q8PZ66

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program