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Q8PYZ8 (HPPA1_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
K(+)-stimulated pyrophosphate-energized sodium pump
EC=3.6.1.1
Alternative name(s):
Membrane-bound sodium-translocating pyrophosphatase
Mm-PPase
Pyrophosphate-energized inorganic pyrophosphatase
Short name=Na(+)-PPase
Gene names
Name:hppA1
Ordered Locus Names:MM_0700
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length676 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na+ movement across the membrane. Ref.3

Catalytic activity

Diphosphate + H2O = 2 phosphate. Ref.3

Cofactor

Magnesium. Ref.3

Enzyme regulation

Requires K+ for maximal activity. Inhibited by the Na+ ionophore monensin, activated by the K+ ionophore valinomycin and unaffected by the protonophore CCCP. Ref.3

Subunit structure

Homodimer By similarity. HAMAP MF_01129

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.3.

Sequence similarities

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. [View classification]

Biophysicochemical properties

pH dependence:

Optimum pH is 7.5. Ref.3

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 676676K(+)-stimulated pyrophosphate-energized sodium pump HAMAP MF_01129
PRO_0000217007

Regions

Transmembrane3 – 2321Helical; Potential
Transmembrane58 – 7821Helical; Potential
Transmembrane83 – 10321Helical; Potential
Transmembrane129 – 14921Helical; Potential
Transmembrane158 – 17821Helical; Potential
Transmembrane241 – 26121Helical; Potential
Transmembrane265 – 28521Helical; Potential
Transmembrane298 – 31821Helical; Potential
Transmembrane324 – 34421Helical; Potential
Transmembrane390 – 41021Helical; Potential
Transmembrane411 – 43121Helical; Potential
Transmembrane469 – 48921Helical; Potential
Transmembrane501 – 52121Helical; Potential
Transmembrane572 – 59221Helical; Potential
Transmembrane593 – 61321Helical; Potential
Transmembrane656 – 67621Helical; Potential

Sites

Site4631Determinant of potassium dependence By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8PYZ8 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 36DFDBDA732A9AD8

FASTA67669,221
        10         20         30         40         50         60 
MDMLIYLTPI CALIGLIFAG ISYKNVRNEG EGNELIKKIT AAIHGGAMVY LNRQYRAIAV 

        70         80         90        100        110        120 
FVVFIAIVLA LVLPNGVLTA ACFVFGAVLS ATAGYAGMLT ATIANGRTTN AATRGIGPAF 

       130        140        150        160        170        180 
KVSFASGTVM GMSVVGLGLF GLSLSFIILG NIYTDMDLFT LLNIIAGFSF GASSIALFAR 

       190        200        210        220        230        240 
VGGGIFTKAA DVGADLVGKV EAGIPEDDPR NPAVIADNVG DNVGDIAGMG ADLYESYVGS 

       250        260        270        280        290        300 
IIATMLLAAS TAAATFPGIP VMNVVMVPLV IAAVGILASV IGTFFVRTNK TESSAIHMAF 

       310        320        330        340        350        360 
NMGLIAAIVL TVIASYVVTG HLLGGYGLNV FFSTVAGLVA GFLIGQITEH YTSYDKKPTL 

       370        380        390        400        410        420 
TVAHSCQTGS ATNVITGFAK GMESTLWPAV IISIAIYIAF QLAGLYGIAI AAVGMLATLG 

       430        440        450        460        470        480 
ISLSVDAYGP VADNAGGIAE MSHQKKEVRQ ITDTLDAVGN TTAAMGKGFA IGSAALTALS 

       490        500        510        520        530        540 
LFASYAIAVG LTSIDVMNPN VFIGLIIGAM LPFLFSSMTI LAVGNAAGEV VVEVRRQFKE 

       550        560        570        580        590        600 
IKGLMEGKAD PDYSKCITIS THSALKEMIP PGILAVIAPI LVGLVLGAGA LGGLLAGSVV 

       610        620        630        640        650        660 
SGFMLAITMS NAGGSWDNAK KFIELGNFGG KGSDAHKAGV TGDTVGDPFK DTAGPAINIL 

       670 
IKLMSIVALV FAPLFI

« Hide

References

« Hide 'large scale' references
[1]"Identification and analysis of proton-translocating pyrophosphatases in the methanogenic archaeon Methanosarcina mazei."
Baeumer S., Lentes S., Gottschalk G., Deppenmeier U.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.
[2]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.
[3]"Na+-pyrophosphatase: a novel primary sodium pump."
Malinen A.M., Belogurov G.A., Baykov A.A., Lahti R.
Biochemistry 46:8872-8878(2007) [PubMed: 17605473] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF312701 Genomic DNA. Translation: AAM22543.1.
AE008384 Genomic DNA. Translation: AAM30396.1.
RefSeqNP_632724.1. NC_003901.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1479042.
GenomeReviewsGene locus MM_0700 in contig AE008384_GR.
KEGGmma:MM_0700.
NMPDRfig|192952.1.peg.700.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG593668.
OMANKTESSA.
PhylomeDBQ8PYZ8.
ProtClustDBPRK00733.

Enzyme and pathway databases

BioCycMMAZ192952:MM0700-MONOMER.

Family and domain databases

HAMAPMF_01129. PPase-energized_pump.
[Tree]
InterProIPR004131. PPase-energised_H-pump.
[Graphical view]
KOK01507.
PfamPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsTIGR01104. V_PPase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHPPA1_METMA
AccessionPrimary (citable) accession number: Q8PYZ8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: October 1, 2002
Last modified: December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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