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Protein

K(+)-stimulated pyrophosphate-energized sodium pump

Gene

hppA1

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na+ movement across the membrane.UniRule annotation1 Publication

Catalytic activityi

Diphosphate + H2O = 2 phosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation1 Publication

Enzyme regulationi

Requires K+ for maximal activity. Inhibited by the Na+ ionophore monensin, activated by the K+ ionophore valinomycin and unaffected by the protonophore CCCP.UniRule annotation1 Publication

pH dependencei

Optimum pH is 7.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei180Important for ion transportBy similarity1
Binding sitei188SubstrateBy similarity1
Metal bindingi191Magnesium 1By similarity1
Metal bindingi195Magnesium 1By similarity1
Metal bindingi218Magnesium 2By similarity1
Metal bindingi221Magnesium 2By similarity1
Sitei225Important for ion transportBy similarity1
Sitei232Important for ion transportBy similarity1
Metal bindingi433Magnesium 2By similarity1
Sitei463Important for potassium dependenceBy similarity1
Metal bindingi617Calcium or magnesiumBy similarity1
Metal bindingi643Calcium or magnesiumBy similarity1
Metal bindingi647Calcium or magnesiumBy similarity1
Binding sitei650SubstrateBy similarity1
Sitei651Important for ion transportBy similarity1
Sitei662Important for ion transportBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Calcium, Magnesium, Metal-binding, Potassium, Sodium

Protein family/group databases

TCDBi3.A.10.1.14. the h(+), na(+)-translocating pyrophosphatase (m(+)-ppase) family.

Names & Taxonomyi

Protein namesi
Recommended name:
K(+)-stimulated pyrophosphate-energized sodium pump (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Membrane-bound sodium-translocating pyrophosphataseUniRule annotation
Mm-PPase
Pyrophosphate-energized inorganic pyrophosphataseUniRule annotation
Short name:
Na(+)-PPaseUniRule annotation
Gene namesi
Name:hppA1UniRule annotation
Ordered Locus Names:MM_0700
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei3 – 23HelicalUniRule annotationAdd BLAST21
Transmembranei58 – 78HelicalUniRule annotationAdd BLAST21
Transmembranei83 – 103HelicalUniRule annotationAdd BLAST21
Transmembranei129 – 149HelicalUniRule annotationAdd BLAST21
Transmembranei158 – 178HelicalUniRule annotationAdd BLAST21
Transmembranei241 – 261HelicalUniRule annotationAdd BLAST21
Transmembranei265 – 285HelicalUniRule annotationAdd BLAST21
Transmembranei298 – 318HelicalUniRule annotationAdd BLAST21
Transmembranei324 – 344HelicalUniRule annotationAdd BLAST21
Transmembranei390 – 410HelicalUniRule annotationAdd BLAST21
Transmembranei411 – 431HelicalUniRule annotationAdd BLAST21
Transmembranei469 – 489HelicalUniRule annotationAdd BLAST21
Transmembranei501 – 521HelicalUniRule annotationAdd BLAST21
Transmembranei572 – 592HelicalUniRule annotationAdd BLAST21
Transmembranei593 – 613HelicalUniRule annotationAdd BLAST21
Transmembranei656 – 676HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002170071 – 676K(+)-stimulated pyrophosphate-energized sodium pumpAdd BLAST676

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi192952.MM_0700.

Family & Domainsi

Sequence similaritiesi

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiarCOG04949. Archaea.
COG3808. LUCA.
HOGENOMiHOG000007098.
KOiK15987.
OMAiQVKNWHL.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8PYZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDMLIYLTPI CALIGLIFAG ISYKNVRNEG EGNELIKKIT AAIHGGAMVY
60 70 80 90 100
LNRQYRAIAV FVVFIAIVLA LVLPNGVLTA ACFVFGAVLS ATAGYAGMLT
110 120 130 140 150
ATIANGRTTN AATRGIGPAF KVSFASGTVM GMSVVGLGLF GLSLSFIILG
160 170 180 190 200
NIYTDMDLFT LLNIIAGFSF GASSIALFAR VGGGIFTKAA DVGADLVGKV
210 220 230 240 250
EAGIPEDDPR NPAVIADNVG DNVGDIAGMG ADLYESYVGS IIATMLLAAS
260 270 280 290 300
TAAATFPGIP VMNVVMVPLV IAAVGILASV IGTFFVRTNK TESSAIHMAF
310 320 330 340 350
NMGLIAAIVL TVIASYVVTG HLLGGYGLNV FFSTVAGLVA GFLIGQITEH
360 370 380 390 400
YTSYDKKPTL TVAHSCQTGS ATNVITGFAK GMESTLWPAV IISIAIYIAF
410 420 430 440 450
QLAGLYGIAI AAVGMLATLG ISLSVDAYGP VADNAGGIAE MSHQKKEVRQ
460 470 480 490 500
ITDTLDAVGN TTAAMGKGFA IGSAALTALS LFASYAIAVG LTSIDVMNPN
510 520 530 540 550
VFIGLIIGAM LPFLFSSMTI LAVGNAAGEV VVEVRRQFKE IKGLMEGKAD
560 570 580 590 600
PDYSKCITIS THSALKEMIP PGILAVIAPI LVGLVLGAGA LGGLLAGSVV
610 620 630 640 650
SGFMLAITMS NAGGSWDNAK KFIELGNFGG KGSDAHKAGV TGDTVGDPFK
660 670
DTAGPAINIL IKLMSIVALV FAPLFI
Length:676
Mass (Da):69,221
Last modified:October 1, 2002 - v1
Checksum:i36DFDBDA732A9AD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF312701 Genomic DNA. Translation: AAM22543.1.
AE008384 Genomic DNA. Translation: AAM30396.1.
RefSeqiWP_011032651.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM30396; AAM30396; MM_0700.
GeneIDi24880497.
KEGGimma:MM_0700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF312701 Genomic DNA. Translation: AAM22543.1.
AE008384 Genomic DNA. Translation: AAM30396.1.
RefSeqiWP_011032651.1. NC_003901.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_0700.

Protein family/group databases

TCDBi3.A.10.1.14. the h(+), na(+)-translocating pyrophosphatase (m(+)-ppase) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM30396; AAM30396; MM_0700.
GeneIDi24880497.
KEGGimma:MM_0700.

Phylogenomic databases

eggNOGiarCOG04949. Archaea.
COG3808. LUCA.
HOGENOMiHOG000007098.
KOiK15987.
OMAiQVKNWHL.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHPPA1_METMA
AccessioniPrimary (citable) accession number: Q8PYZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.