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Q8PYZ7 (HPPA2_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
K(+)-insensitive pyrophosphate-energized proton pump
EC=3.6.1.1
Alternative name(s):
Membrane-bound proton-translocating pyrophosphatase
Pyrophosphate-energized inorganic pyrophosphatase
Short name=H(+)-PPase
Gene names
Name:hppA2
Ordered Locus Names:MM_0701
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length671 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force By similarity. HAMAP MF_01129

Catalytic activity

Diphosphate + H2O = 2 phosphate. HAMAP MF_01129

Cofactor

Magnesium By similarity. HAMAP MF_01129

Subunit structure

Homodimer By similarity. HAMAP MF_01129

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_01129.

Sequence similarities

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily. [View classification]

Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandMagnesium
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processproton transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionhydrogen-translocating pyrophosphatase activity

Inferred from electronic annotation. Source: InterPro

inorganic diphosphatase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 671671K(+)-insensitive pyrophosphate-energized proton pump HAMAP MF_01129
PRO_0000217037

Regions

Transmembrane4 – 2421Helical; Potential
Transmembrane57 – 7721Helical; Potential
Transmembrane79 – 9921Helical; Potential
Transmembrane128 – 14821Helical; Potential
Transmembrane156 – 17621Helical; Potential
Transmembrane223 – 24321Helical; Potential
Transmembrane249 – 26921Helical; Potential
Transmembrane285 – 30521Helical; Potential
Transmembrane310 – 33021Helical; Potential
Transmembrane365 – 38521Helical; Potential
Transmembrane393 – 41321Helical; Potential
Transmembrane452 – 47221Helical; Potential
Transmembrane490 – 51021Helical; Potential
Transmembrane558 – 57821Helical; Potential
Transmembrane579 – 59921Helical; Potential
Transmembrane650 – 67021Helical; Potential

Sites

Site4511Determinant of potassium independence By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8PYZ7 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 3179C2EF48EF45C8

FASTA67169,062
        10         20         30         40         50         60 
MERLIFTAPL AGLISLTFAA FFAKSILKED AGNKRMKEIA EAIKEGSTAY MKRQYRTIAV 

        70         80         90        100        110        120 
VSVIISLLIL FLLDEGLKIA AGFLAGAISS AAAGYIGMSI SVRANVRTAS AASGGAGKAL 

       130        140        150        160        170        180 
KIAFRGGAVT GLAVIGLALL GTSSLYILYG DADLVVGFGF GASLISLFAR AGGGIFTKAA 

       190        200        210        220        230        240 
DVGADLVGKI EAGIPEDDPR NPAVIADNVG DNVGDCAGMG ADLFETYVVT SLAAMLLGSL 

       250        260        270        280        290        300 
IIGTYKNAVL YPLMLGSAAI FASIISVFFV KVEKEGKVMS ALYRGVGGST VLSLIAFYYI 

       310        320        330        340        350        360 
TGFLMGDSRF FYVTVAGVVI TVLMVIVTEY YTSKSCRPVK TIAVSSETGA ATNIISGLSA 

       370        380        390        400        410        420 
GFESTLVPAV VIAAGILVSY FIVGGSADPG TGLYGIAIAS VAMLSTAGMI VALDSYGPIT 

       430        440        450        460        470        480 
DNAGGIAQMA NLPAQVRKVT DELDSVGNTT KAVTKGYAIG STALGALALF ADYRNKVSLE 

       490        500        510        520        530        540 
SQSISLDSPV VLSGILLGAV LPFLFSAVMM SAVGKAAFEV VNEVRRQFRE IPGIMEGTAK 

       550        560        570        580        590        600 
PEYGRCVDIV TKAALHDMAM PGFLAVIIPL LTGFFLGPEA LAGLLTGLIV VGFMLALMMD 

       610        620        630        640        650        660 
NGGGAWDNAK KLIEDGYYGG KGSEAHRAAV VGDTVGDPFK DTAGPALNSL IKVVNMVAIL 

       670 
FSPLIIGGGF L

« Hide

References

« Hide 'large scale' references
[1]"Identification and analysis of proton-translocating pyrophosphatases in the methanogenic archaeon Methanosarcina mazei."
Baeumer S., Lentes S., Gottschalk G., Deppenmeier U.
Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.
[2]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF312701 Genomic DNA. Translation: AAM22542.1.
AE008384 Genomic DNA. Translation: AAM30397.1.
RefSeqNP_632725.1. NC_003901.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

TCDB3.A.10.2.4. H+-translocating pyrophosphatase (H+-PPase) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1479043.
GenomeReviewsGene locus MM_0701 in contig AE008384_GR.
KEGGmma:MM_0701.
NMPDRfig|192952.1.peg.701.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG593668.
OMAILEWKEQ.
PhylomeDBQ8PYZ7.
ProtClustDBPRK00733.

Enzyme and pathway databases

BioCycMMAZ192952:MM0701-MONOMER.

Family and domain databases

HAMAPMF_01129. PPase-energized_pump.
[Tree]
InterProIPR004131. PPase-energised_H-pump.
[Graphical view]
KOK01507.
PfamPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsTIGR01104. V_PPase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHPPA2_METMA
AccessionPrimary (citable) accession number: Q8PYZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: October 1, 2002
Last modified: December 14, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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