Q8PYK1 (PURL_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	MM_0860

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	716 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.
Sequence caution	The sequence AAM30556.1 differs from that shown. Reason: Frameshift at position 26.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 716	716	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100515
Regions
	Nucleotide binding	90 – 101	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8PYK1 [UniParc]. Last modified November 8, 2002. Version 2. Checksum: 507E9EA5B961D880	FASTA	716	76,311
	10 20 30 40 50 60 MLPEEDLKII KKELGREPTL VEQGCFLNLW SEHCSYRSSA PLLKTFTTTG ENVIIGPGDD 70 80 90 100 110 120 AAIIKFEDGY VLAIGMESHN HPSYVDPYNG AATGVGGIVR DIISMGARPI ALMDPLYFGP 130 140 150 160 170 180 LDTPKNLFLF EQIIKGIAGY GNCIGVPVVN GETFFDRRYS GNPLVNVVAV GLCKEEKVMT 190 200 210 220 230 240 SRSQKAGNKL ILAGSSTGKD GLGGASFASR DLSESAEAED RPSVQVGDPY TEKLVMEMTL 250 260 270 280 290 300 EAIDKGYIKS CKDLGAAGLG GASSELAAKG GLGAYIIADA VPQREPEMNA YEILLAESQE 310 320 330 340 350 360 RMVFEVAPED VDAVLALVQK YDLNGAVIGH LTEKPNYTVE FRGEIVADIP IGFLTGGAPT 370 380 390 400 410 420 CEKPSEAPIH REEGKKPETP EDLKAAFMKV LSSHNIASKE WIYRQYDHEV QLRTVVKPGE 430 440 450 460 470 480 DSGVLRITDK KGIALSCGCQ PRATLLDPYT GGRTAIIENA MNLAVKGAEG LAIVNCLNFG 490 500 510 520 530 540 NPENPETYWQ FKNAVLGLGD AARELSIPVV GGNVSLYNES DEFRTAIPPT PSIGMIGKVD 550 560 570 580 590 600 LETPLPSGFF AKTGDSIILV GETVPEMGGS EYYSCMGAGN AGKVPEVPKN APEIIKAVIE 610 620 630 640 650 660 AVKSGKLNSA HDISLGGIGA GLARMCKNMG GKVDVSEIAG GMKEDEFLFS EAPARALLAT 670 680 690 700 710 AEPEAVQELL KGVPHAVIGT VGGDALEIKG KDFELFISLE EIKNAHESLT KFMMMG

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM30556.1. Frameshift.
RefSeq	NP_632884.1. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PYK1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1479202.
GenomeReviews	Gene locus MM_0860 in contig AE008384_GR.
KEGG	mma:MM_0860.
NMPDR	fig|192952.1.peg.860.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q8PYK1.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM0860-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_METMA

Accession

Primary (citable) accession number: Q8PYK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	November 8, 2002
Last modified:	January 25, 2012
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program