Reviewed, UniProtKB/Swiss-Prot Q8PY84 (HDRC_METMA)

Names and origin

Protein names

Recommended name:
    CoB--CoM heterodisulfide reductase 1 iron-sulfur subunit C
    EC=1.8.98.1

Gene names

Name:	hdrC
Ordered Locus Names:	MM_0979

Organism

Methanosarcina mazei (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

2209 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B) By similarity.
Catalytic activity	Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine.
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Pathway	Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.
Subunit structure	The heterodisulfide reductase 1 is composed of three subunits; hdrA, hdrB and hdrC By similarity.
Sequence similarities	Belongs to the hdrC family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Methanogenesis
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cofactor metabolic process Inferred from electronic annotation. Source: InterPro methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW CoB--CoM heterodisulfide reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	CoB--CoM heterodisulfide reductase 1 iron-sulfur subunit C		PRO_0000150076
Regions
	Domain	10 – 40	31	4Fe-4S ferredoxin-type 1
	Domain	51 – 82	32	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	19	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	22	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	25	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	29	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	62	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	65	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	68	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	72	1	Iron-sulfur 1 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8PY84-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 40F05C2B90FC23BA	FASTA	161	18,120
	10 20 30 40 50 60 MSEELLKVLK AAGLDVLSCM HCGTCTGSCP SGRHTGLNTR RIIRDARKNR ATVLSDDALW 70 80 90 100 110 120 LCTTCYTCQE RCPRGIPITD ALLELRRLAV RKGFMRPEHR RVSELVLECG HAVPLDEETK 130 140 150 160 KKREELGLDP IPETVQKYPE ALEELKALLK TCKFDELAAE K

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008384 Genomic DNA. Translation: AAM30675.1.
RefSeq	NP_633003.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1479321.
GenomeReviews	Gene locus MM_0979 in contig AE008384_GR.
KEGG	mma:MM_0979.
NMPDR	fig|192952.1.peg.979.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8PY84.
OMA	GLNTRRI.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM0979-MON.
BRENDA	1.8.98.1. 261165.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR017680. CoB/CoM_hetero-S_Rdtase_csu. IPR012285. Fum_reductase_C. [Graphical view]
Gene3D	G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMs	TIGR03290. CoB_CoM_SS_C. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HDRC_METMA

Accession

Primary (citable) accession number: Q8PY84

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)