Reviewed, UniProtKB/Swiss-Prot Q8PY83 (HDRB_METMA)

Names and origin

Protein names

Recommended name:
    CoB--CoM heterodisulfide reductase 1 subunit B
    EC=1.8.98.1

Gene names

Name:	hdrB
Ordered Locus Names:	MM_0980

Organism

Methanosarcina mazei (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

2209 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B). HdrB may have a function in energy transduction By similarity.
Catalytic activity	Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine.
Pathway	Cofactor metabolism; coenzyme B/coenzyme M regeneration; coenzyme B and coenzyme M from CoB-CoM heterodisulfide: step 1/1.
Subunit structure	The heterodisulfide reductase 1 is composed of three subunits; hdrA, hdrB and hdrC By similarity.
Sequence similarities	Belongs to the hdrB family.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cofactor metabolic process Inferred from electronic annotation. Source: InterPro methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	CoB--CoM heterodisulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	CoB--CoM heterodisulfide reductase 1 subunit B		PRO_0000150068

Sequences

	Sequence		Length	Mass (Da)
	Q8PY83-1 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: B694E9152DE0A3DE	FASTA	300	33,068
	10 20 30 40 50 60 MNKLSLFLGC IVPNRYPGIE KATKLCLQRL EIDACDLPGA SCCPAPGVFK SFDKATWLAL 70 80 90 100 110 120 ASRNIVLSER MERDVLTVCN GCYGSLADAN MELKKDPEMK ACTNSCLKEI GMEFRGTSEI 130 140 150 160 170 180 RHIIEFLYKE FGPEKLKEYI TTPLDLKVAL HYGCHLIKPS KDRNLGDTES PVFFDELVEA 190 200 210 220 230 240 TGAKSVDYTD KMMCCGAGGG VRSGYADESL EMLEHKLDCI CKAGVDCIVN ACPFCHLQFD 250 260 270 280 290 300 RGQIAVNEKF GTDYSIPVLH YSQLLGLALG FSPDQLGIEQ NAVQNIEFLA KIYEISAGLS

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008384 Genomic DNA. Translation: AAM30676.1. Different initiation.
RefSeq	NP_633004.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1479322.
GenomeReviews	Gene locus MM_0980 in contig AE008384_GR.
KEGG	mma:MM_0980.
NMPDR	fig|192952.1.peg.980.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8PY83.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM0980-MON.
BRENDA	1.8.98.1. 261165.
Family and domain databases
InterPro	IPR017678. CoB/CoM_hetero-S_Rdtase_bsu. IPR004017. Cys_rich_region. [Graphical view]
Pfam	PF02754. CCG. 2 hits. [Graphical view]
TIGRFAMs	TIGR03288. CoB_CoM_SS_B. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HDRB_METMA

Accession

Primary (citable) accession number: Q8PY83

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	June 16, 2009
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)