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Protein

Proteasome-activating nucleotidase

Gene

pan

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei342 – 3421ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi203 – 2086ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-1032-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome-activating nucleotidaseUniRule annotation
Short name:
PANUniRule annotation
Alternative name(s):
Proteasomal ATPaseUniRule annotation
Proteasome regulatory ATPaseUniRule annotation
Proteasome regulatory particleUniRule annotation
Gene namesi
Name:panUniRule annotation
Ordered Locus Names:MM_1006
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420Proteasome-activating nucleotidasePRO_0000084745Add
BLAST

Interactioni

Subunit structurei

Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.UniRule annotation

Protein-protein interaction databases

STRINGi192952.MM_1006.

Structurei

3D structure databases

ProteinModelPortaliQ8PY58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni418 – 4203Docks into pockets in the proteasome alpha-ring to cause gate openingUniRule annotation

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili22 – 7958UniRule annotationAdd
BLAST

Domaini

Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.UniRule annotation

Sequence similaritiesi

Belongs to the AAA ATPase family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiarCOG01306. Archaea.
COG1222. LUCA.
HOGENOMiHOG000225143.
KOiK03420.
OMAiMPGIEAR.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00553. PAN.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR023501. Nucleotidase_PAN.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8PY58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSHLVKPGS VYDGIEPGEL GETTESVQDR VRQLESRNSF LEEQCSQIES
60 70 80 90 100
EKRYLENQKI KYEREIRKLQ SELDRMKTSP LIIGTVIDVI KNDRIIVRSS
110 120 130 140 150
NGPQFLVNVS QYIDEKKLLP GAKVALNQHT LAIAEVIPST EEPFVAAMEV
160 170 180 190 200
IESVEVDYDQ IGGLDEQIQE LQEAVELPLT SPERFARIGI EPPKGVLLYG
210 220 230 240 250
LPGTGKTLLA KAVAHRTNAT FIRVVGSELV QKYIGDGSKL VREIFEMARK
260 270 280 290 300
KAPSIIFIDE LDSIAARRLN ETTGADREVQ RTLMQLLAEM DGFDKRKNIR
310 320 330 340 350
IIAATNRPDV LDPAILRPGR FDRLVHVPMP GIEARGKILK IHCEKMTLAE
360 370 380 390 400
DIDFKKLAKA TEGMSGADLK AIATEAGMFA VRRDKELVEM EEFLEAVEKV
410 420
SMAADTQKMM PSNLPETMFV
Length:420
Mass (Da):47,056
Last modified:October 10, 2002 - v1
Checksum:i6F0501BA2D7B72D5
GO

Sequence cautioni

The sequence AAM30702.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM30702.1. Different initiation.

Genome annotation databases

EnsemblBacteriaiAAM30702; AAM30702; MM_1006.
KEGGimma:MM_1006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM30702.1. Different initiation.

3D structure databases

ProteinModelPortaliQ8PY58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_1006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM30702; AAM30702; MM_1006.
KEGGimma:MM_1006.

Phylogenomic databases

eggNOGiarCOG01306. Archaea.
COG1222. LUCA.
HOGENOMiHOG000225143.
KOiK03420.
OMAiMPGIEAR.

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-1032-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00553. PAN.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR023501. Nucleotidase_PAN.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
    Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R.
    , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Entry informationi

Entry nameiPAN_METMA
AccessioniPrimary (citable) accession number: Q8PY58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: October 10, 2002
Last modified: November 11, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.