Reviewed, UniProtKB/Swiss-Prot Q8PXV8 (MTD_METMA)

Names and origin

Protein names

Recommended name:
    F420-dependent methylenetetrahydromethanopterin dehydrogenase
      Short name=MTD
    EC=1.5.99.9
Alternative name(s):
    Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase

Gene names

Name:	mtd
Ordered Locus Names:	MM_1108

Organism

Methanosarcina mazei (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

2209 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reduction of methenyl-H4MPT+ to methylene-H4MPT By similarity. HAMAP MF_00058
Catalytic activity	5,10-methylenetetrahydromethanopterin + coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_00058
Pathway	One-carbon metabolism; methanogenesis from CO(2); 5,10-methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin (coenzyme F420 route): step 1/1. HAMAP MF_00058
Sequence similarities	Belongs to the MTD family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro methylenetetrahydromethanopterin dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	F420-dependent methylenetetrahydromethanopterin dehydrogenase HAMAP MF_00058		PRO_0000075040

Sequences

	Sequence		Length	Mass (Da)
	Q8PXV8-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: F52747A8B98723DE	FASTA	279	30,240
	10 20 30 40 50 60 MKKMVNIGFI KMGNLGMSQV INLIQDEIAA REGITVRVFG TGAKMAPADA ADTESFKQWN 70 80 90 100 110 120 ADFVVIISPN AAAPGPTAAR EIWKDVPCII VSDGPTKKEA REAFEQEGFG YIILPVDPLI 130 140 150 160 170 180 GAKREFLDSV EMASFNADAM KVLSICGVVR LIQEELDKVT EQVASGKSGK DLELPHIFAK 190 200 210 220 230 240 PEKCVEHAGF ANPYAKSKAL AALHMAEKVA QVNFPACFML KEIEQVCLTA AAGHEIMGAA 250 260 270 AQLANQAREI EKSNDTVSRM PHAKNGAVLK KVRLYEKPE

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM30804.1.
RefSeq	NP_633132.1.
3D structure databases
SMR	Q8PXV8. Positions 4-279.
ModBase	Search...
Genome annotation databases
GeneID	1479450.
GenomeReviews	Gene locus MM_1108 in contig AE008384_GR.
KEGG	mma:MM_1108.
NMPDR	fig|192952.1.peg.1108.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG538567.
OMA	IVASAHE.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM1108-MONOMER.
BRENDA	1.5.99.9. 261165.
Family and domain databases
HAMAP	MF_00058. MTD. [Tree]
InterPro	IPR002844. Methylene_DH. [Graphical view]
Pfam	PF01993. MTD. 1 hit. [Graphical view]
PIRSF	PIRSF005627. MTD. 1 hit.
ProDom	PD017604. Methylene_DH. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

MTD_METMA

Accession

Primary (citable) accession number: Q8PXV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	October 1, 2002
Last modified:	January 19, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)