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Q8PXL7 (DAPA_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:MM_1201
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Dihydrodipicolinate synthase HAMAP MF_00418
PRO_0000103199

Regions

Region48 – 492Pyruvate binding By similarity

Sites

Active site1591Schiff-base intermediate with substrate By similarity
Binding site1041Pyruvate By similarity
Site1311Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8PXL7 [UniParc].

Last modified October 19, 2002. Version 1.
Checksum: 45BF0B0D017FFA26

FASTA29130,704
        10         20         30         40         50         60 
MFEGAMPALI TPFTKDDRID REGLQRNIAF VEEGGVSGIV PCGTTGESAT LSAAEHEEVI 

        70         80         90        100        110        120 
DIAVECSKVP VIAGTGSNNT GEALQFTKHA ADAGVDGVLL ISPYYNKPNP AGLLAHFKKI 

       130        140        150        160        170        180 
AEAVDIPMIL YNVPSRTGQD MPVDVIVELA KVENIVGIKE ASGNAAKVSQ ILENTMDDDF 

       190        200        210        220        230        240 
VVLSGEDGLT LPIISMGGRG VISVAANIVP DKMSGMVNAA LKGDYETARK LHFEIAPLIR 

       250        260        270        280        290 
ALFLETNPIP VKKAAELVGL ASGHLRLPLA PISDANQAKL ANELRKLGVM E

« Hide

References

[1]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE008384 Genomic DNA. Translation: AAM30897.1.
RefSeqNP_633225.1. NC_003901.1.

3D structure databases

ProteinModelPortalQ8PXL7.
SMRQ8PXL7. Positions 1-290.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1479543.
GenomeReviewsGene locus MM_1201 in contig AE008384_GR.
KEGGmma:MM_1201.
NMPDRfig|192952.1.peg.1201.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG358848.
OMACEMEDSN.
PhylomeDBQ8PXL7.
ProtClustDBPRK03170.

Enzyme and pathway databases

BioCycMMAZ192952:MM1201-MONOMER.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_METMA
AccessionPrimary (citable) accession number: Q8PXL7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: December 14, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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