Reviewed, UniProtKB/Swiss-Prot Q8PXK0 (GUAAB_METMA)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit B
    EC=6.3.5.2
Alternative name(s):
    GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	MM_1218

Organism

Methanosarcina mazei (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

2209 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_0000140243
Regions
	Domain	33 – 185	153	GMP-binding
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PXK0-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 61BE50537935988D	FASTA	305	34,117
	10 20 30 40 50 60 MVKPEKFIPK AIEKISKEIK DGKAIIALSG GVDSSVCAEL AYRAIGDRLQ PIYIDTGLMR 70 80 90 100 110 120 KGETERIRHI FSHMNLDVVY AKDRFLAALA GVTDPEEKRK AIGETFIRVF EEEARKLAAD 130 140 150 160 170 180 YLIQGTIYPD RIESEGGIKS HHNVGGLPSV MDFKKIVEPI EDLYKDEVRE VAWALQLPDE 190 200 210 220 230 240 ICERMPFPGP GLAVRVLGEV TEEKLEVARE ANFIVEEELL ERFCPWQTFA AVLGKGTGVK 250 260 270 280 290 300 GDVRAYGWIV AIRAVGSRDG MTAEALELPW EVLKHLESRI TSEIPKVARV VYDITPKPPA TIEFE

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008384 Genomic DNA. Translation: AAM30914.1.
RefSeq	NP_633242.1.
3D structure databases
HSSP	HSSP built from PDB template 1GPM based on UniProtKB P04079.
ModBase	Search...
Genome annotation databases
GeneID	1479560.
GenomeReviews	Gene locus MM_1218 in contig AE008384_GR.
KEGG	mma:MM_1218.
NMPDR	fig|192952.1.peg.1218.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8PXK0.
OMA	GIKSHHN.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM1218-MON.
BRENDA	6.3.5.2. 261165.
Family and domain databases
HAMAP	MF_00345. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR018317. Queuosine_synth-like. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF06508. ExsB. 1 hit. PF00958. GMP_synt_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GUAAB_METMA

Accession

Primary (citable) accession number: Q8PXK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)