Reviewed, UniProtKB/Swiss-Prot Q8PXH2 (DP2L_METMA)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	MM_1246

Organism

Methanosarcina mazei (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

2209 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	1152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1152	1152	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000152577

Sequences

	Sequence		Length	Mass (Da)
	Q8PXH2-1 [UniParc]. Last modified October 19, 2002. Version 1. Checksum: 48FDEEBAB8546737	FASTA	1,152	128,786
	10 20 30 40 50 60 MGETIASEEM HRYFDGLEAR LKEAIEIANR ARARGGDPRP VVEIPLAKDL ADRVENLIGV 70 80 90 100 110 120 KGVAEKIREL EARMSREEAA LEIGKQVAEG VVGSFPSKKD AVEAAIRVSM AVLTEGVVAA 130 140 150 160 170 180 PIEGIDKVDL GKNDDGSQYI RIFYSGPIRS AGGTAQALSV LVGDYVRRGI GIDRYKPREE 190 200 210 220 230 240 EVERYVEEIL LYKRVASLQY TPSEDEIRLI VRNCPVCIDG DPTEEAEVEG HRDLERIGTN 250 260 270 280 290 300 RVRGGMCLVL AEGLALKAPK VKKHVNKLKM DGWDWLETLI GGAKSGESDE DEQKNKIKPK 310 320 330 340 350 360 DKYIRDLIAG RPVFSHPSRP GGFRLRYGRS RNTSFASAGI NPAGMVLLDD FITNGTQLKI 370 380 390 400 410 420 ERPGKAAAMS AVDSIEGPTV RLFSGDLIRV DDIKEAYEVR QQVEVIVDIG EILINYGDFL 430 440 450 460 470 480 ENNHPLMPSP YVFEWWIYDY ESVCSEKILE KDLKNPSASL ALKLAEKYNV PLHPKFTYLW 490 500 510 520 530 540 HDINRNEFEA LRKFVAEKGT FLEGEGEGEG EGILKLPLED SVKEGIKPVL EKLLVLHKVK 550 560 570 580 590 600 EGGIFVEEAL PFILCLGLDR SLKEKVSMPD TGDMVEAAGI LSGFKVYPKA PSRIGARMGR 610 620 630 640 650 660 PEKSDLRKMS PAAQVLFPIS NAGGMTRNLV SASDYTSCMN AKIGEIEVEL GLRECPACGK 670 680 690 700 710 720 ESYFWRCECG EFTNPKLSCP RCKIDVRGAE TCPKCGRKPT SVANVKLDFR PIYKQAFENV 730 740 750 760 770 780 GERERMDIIK GVKRLMNGQM TPEPLEKGIL RAKHDVYIFK DGTVRYDMSD IPLTHIRADE 790 800 810 820 830 840 LGITAARLRE LDYKEDIYGK PLERDDQVVC LKVQDLLLSY DGAEYMLRTA KYVDELLVKY 850 860 870 880 890 900 YKVEPYYNAE TIQDLVGVLM IGLAPHTSAG VLGRLIGFTK ASVGYAHPFF HASKRRNCDG 910 920 930 940 950 960 DEDCVMLLMD GILNFSRSYL PEKRGGKMDA PLVLTTRIDP KEVDKEAHNI DLLARYPLEF 970 980 990 1000 1010 1020 YRATQEIKNP TEIESIMDLV SSRLGKPDQY EHFMFTHDTS NIAAGPLKSS YKTLGSMIEK 1030 1040 1050 1060 1070 1080 MEAQLSLAGK IRAVDAPDVA ERVLKSHFLP DLIGNLRSFS RQRMRCIKCG EKFRRPPLTG 1090 1100 1110 1120 1130 1140 ACPKCGGNVV LTVHEGAVRK YLEISKEIGE RYGVSSYTRQ RIELLDYDIC SLFENHKVKQ 1150 LGLSDFMSGS AR

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008384 Genomic DNA. Translation: AAM30942.1.
RefSeq	NP_633270.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1479588.
GenomeReviews	Gene locus MM_1246 in contig AE008384_GR.
KEGG	mma:MM_1246.
NMPDR	fig|192952.1.peg.1246.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8PXH2.
OMA	Q8PXH2. NCDGDED.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM1246-MON.
BRENDA	2.7.7.7. 261165.
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METMA

Accession

Primary (citable) accession number: Q8PXH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 19, 2002
Last modified:	May 5, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)