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Protein

DNA polymerase II large subunit

Gene

polC

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation
Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-1276-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase II large subunitUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IIUniRule annotation
Gene namesi
Name:polCUniRule annotation
Ordered Locus Names:MM_1246
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11521152DNA polymerase II large subunitPRO_0000152577Add
BLAST

Interactioni

Subunit structurei

Heterodimer of a large subunit and a small subunit.UniRule annotation

Protein-protein interaction databases

STRINGi192952.MM_1246.

Family & Domainsi

Sequence similaritiesi

Belongs to the archaeal DNA polymerase II family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04447. Archaea.
COG1933. LUCA.
HOGENOMiHOG000286435.
KOiK02322.
OMAiRNCDGDE.

Family and domain databases

HAMAPiMF_00324. DNApol_II_L_arch.
InterProiIPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view]
PfamiPF03833. PolC_DP2. 1 hit.
[Graphical view]
PIRSFiPIRSF016275. PolC_DP2. 1 hit.
TIGRFAMsiTIGR00354. polC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8PXH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGETIASEEM HRYFDGLEAR LKEAIEIANR ARARGGDPRP VVEIPLAKDL
60 70 80 90 100
ADRVENLIGV KGVAEKIREL EARMSREEAA LEIGKQVAEG VVGSFPSKKD
110 120 130 140 150
AVEAAIRVSM AVLTEGVVAA PIEGIDKVDL GKNDDGSQYI RIFYSGPIRS
160 170 180 190 200
AGGTAQALSV LVGDYVRRGI GIDRYKPREE EVERYVEEIL LYKRVASLQY
210 220 230 240 250
TPSEDEIRLI VRNCPVCIDG DPTEEAEVEG HRDLERIGTN RVRGGMCLVL
260 270 280 290 300
AEGLALKAPK VKKHVNKLKM DGWDWLETLI GGAKSGESDE DEQKNKIKPK
310 320 330 340 350
DKYIRDLIAG RPVFSHPSRP GGFRLRYGRS RNTSFASAGI NPAGMVLLDD
360 370 380 390 400
FITNGTQLKI ERPGKAAAMS AVDSIEGPTV RLFSGDLIRV DDIKEAYEVR
410 420 430 440 450
QQVEVIVDIG EILINYGDFL ENNHPLMPSP YVFEWWIYDY ESVCSEKILE
460 470 480 490 500
KDLKNPSASL ALKLAEKYNV PLHPKFTYLW HDINRNEFEA LRKFVAEKGT
510 520 530 540 550
FLEGEGEGEG EGILKLPLED SVKEGIKPVL EKLLVLHKVK EGGIFVEEAL
560 570 580 590 600
PFILCLGLDR SLKEKVSMPD TGDMVEAAGI LSGFKVYPKA PSRIGARMGR
610 620 630 640 650
PEKSDLRKMS PAAQVLFPIS NAGGMTRNLV SASDYTSCMN AKIGEIEVEL
660 670 680 690 700
GLRECPACGK ESYFWRCECG EFTNPKLSCP RCKIDVRGAE TCPKCGRKPT
710 720 730 740 750
SVANVKLDFR PIYKQAFENV GERERMDIIK GVKRLMNGQM TPEPLEKGIL
760 770 780 790 800
RAKHDVYIFK DGTVRYDMSD IPLTHIRADE LGITAARLRE LDYKEDIYGK
810 820 830 840 850
PLERDDQVVC LKVQDLLLSY DGAEYMLRTA KYVDELLVKY YKVEPYYNAE
860 870 880 890 900
TIQDLVGVLM IGLAPHTSAG VLGRLIGFTK ASVGYAHPFF HASKRRNCDG
910 920 930 940 950
DEDCVMLLMD GILNFSRSYL PEKRGGKMDA PLVLTTRIDP KEVDKEAHNI
960 970 980 990 1000
DLLARYPLEF YRATQEIKNP TEIESIMDLV SSRLGKPDQY EHFMFTHDTS
1010 1020 1030 1040 1050
NIAAGPLKSS YKTLGSMIEK MEAQLSLAGK IRAVDAPDVA ERVLKSHFLP
1060 1070 1080 1090 1100
DLIGNLRSFS RQRMRCIKCG EKFRRPPLTG ACPKCGGNVV LTVHEGAVRK
1110 1120 1130 1140 1150
YLEISKEIGE RYGVSSYTRQ RIELLDYDIC SLFENHKVKQ LGLSDFMSGS

AR
Length:1,152
Mass (Da):128,786
Last modified:October 19, 2002 - v1
Checksum:i48FDEEBAB8546737
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM30942.1.
RefSeqiWP_011033195.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM30942; AAM30942; MM_1246.
GeneIDi1479588.
KEGGimma:MM_1246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM30942.1.
RefSeqiWP_011033195.1. NC_003901.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_1246.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM30942; AAM30942; MM_1246.
GeneIDi1479588.
KEGGimma:MM_1246.

Phylogenomic databases

eggNOGiarCOG04447. Archaea.
COG1933. LUCA.
HOGENOMiHOG000286435.
KOiK02322.
OMAiRNCDGDE.

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-1276-MONOMER.

Family and domain databases

HAMAPiMF_00324. DNApol_II_L_arch.
InterProiIPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view]
PfamiPF03833. PolC_DP2. 1 hit.
[Graphical view]
PIRSFiPIRSF016275. PolC_DP2. 1 hit.
TIGRFAMsiTIGR00354. polC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
    Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R.
    , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Entry informationi

Entry nameiDP2L_METMA
AccessioniPrimary (citable) accession number: Q8PXH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: November 11, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.