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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei151Proton acceptorUniRule annotation1
Binding sitei153SubstrateUniRule annotation1
Metal bindingi177Magnesium; via carbamate groupUniRule annotation1
Metal bindingi179MagnesiumUniRule annotation1
Metal bindingi180MagnesiumUniRule annotation1
Active sitei270Proton acceptorUniRule annotation1
Binding sitei271SubstrateUniRule annotation1
Binding sitei303SubstrateUniRule annotation1
Sitei310Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase, Oxidoreductase
Biological processCarbon dioxide fixation
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:MM_1249
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000626741 – 428Ribulose bisphosphate carboxylaseAdd BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei177N6-carboxylysineUniRule annotation1

Proteomic databases

PRIDEiQ8PXG9

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Protein-protein interaction databases

STRINGi192952.MM_1249

Structurei

3D structure databases

ProteinModelPortaliQ8PXG9
SMRiQ8PXG9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni354 – 356Substrate bindingUniRule annotation3
Regioni376 – 379Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04443 Archaea
COG1850 LUCA
HOGENOMiHOG000230831
KOiK01601
OMAiHRAMHAA
OrthoDBiPOG093Z02MJ

Family and domain databases

CDDicd08213 RuBisCO_large_III, 1 hit
Gene3Di3.20.20.110, 1 hit
3.30.70.150, 1 hit
HAMAPiMF_01133 RuBisCO_L_type3, 1 hit
InterProiView protein in InterPro
IPR033966 RuBisCO
IPR017712 RuBisCO_III
IPR000685 RuBisCO_lsu_C
IPR036376 RuBisCO_lsu_C_sf
IPR017443 RuBisCO_lsu_fd_N
IPR036422 RuBisCO_lsu_N_sf
PfamiView protein in Pfam
PF00016 RuBisCO_large, 1 hit
PF02788 RuBisCO_large_N, 1 hit
SFLDiSFLDS00014 RuBisCO, 1 hit
SUPFAMiSSF51649 SSF51649, 1 hit
SSF54966 SSF54966, 1 hit
TIGRFAMsiTIGR03326 rubisco_III, 1 hit

Sequencei

Sequence statusi: Complete.

Q8PXG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRDYIDIGY SPKETDLVCE FHIEPTAGVN FEEAATHLAG ESSIDSWTEI
60 70 80 90 100
ATLSPELAEK LKPHVFYADE GAQTVRVAYS EELFELGSVP QVLSAVAGNI
110 120 130 140 150
LSMKIVDNVR LQDIAFPKSM INEFKGPNFG LPGIRKLVGV QDRPLIGTIV
160 170 180 190 200
KPKVGLNSEK HAEVAYNSFV GGCDLVKDDE NLSDQKFNSF EKRAELTLKL
210 220 230 240 250
AEKAESETGE KKMYLCNVTA PTCREMIRRM NFLKDLGASY VMVDIVPAGW
260 270 280 290 300
TAIQTLREEA EDAGLALHAH RCMHSAYTRN PRHGISMLVV AKLCRLIGLD
310 320 330 340 350
QLHIGTVVGK MHGEKHEVLN LRDQCVLDKV PADESQHILA QDWRGLKPMF
360 370 380 390 400
PVASGGLAPT MIPDLYSIFG KDVIMQFGGG IHAHPMGTVA GATACRQALE
410 420
ASLEGISLQD YAKNHKELET ALGKWLKK
Length:428
Mass (Da):47,138
Last modified:October 1, 2002 - v1
Checksum:i89262149EDB15A12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA Translation: AAM30945.1
RefSeqiWP_011033198.1, NC_003901.1

Genome annotation databases

EnsemblBacteriaiAAM30945; AAM30945; MM_1249
GeneIDi24876201
KEGGimma:MM_1249
PATRICifig|192952.21.peg.1455

Similar proteinsi

Entry informationi

Entry nameiRBL_METMA
AccessioniPrimary (citable) accession number: Q8PXG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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