Q8PX31 (SURE_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'-nucleotidase surE
EC=3.1.3.5

Alternative name(s):
Nucleoside 5'-monophosphate phosphohydrolase

Gene names

Name:	surE
Ordered Locus Names:	MM_1391

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates By similarity. HAMAP MF_00060
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP MF_00060
Cofactor	Binds 1 divalent metal cation per subunit By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00060.
Sequence similarities	Belongs to the surE nucleotidase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	5'-nucleotidase surE HAMAP MF_00060		PRO_0000111866
Sites
	Metal binding	14	1	Divalent metal cation By similarity
	Metal binding	15	1	Divalent metal cation By similarity
	Metal binding	45	1	Divalent metal cation By similarity
	Metal binding	100	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PX31 [UniParc]. Last modified October 19, 2002. Version 1. Checksum: 709A83CC7854A771	FASTA	267	29,097
	10 20 30 40 50 60 MGKLMVPKIL VTNDDGVYST GLKAAFDSVS DLGEVTISAP AVQQSGVGRS ISIFEPLRIT 70 80 90 100 110 120 KTDVGGIPAY AVGGTPTDSV ILGVFTILKA MPDLVLSGFN IGENISTDTI TTSGTIGGAL 130 140 150 160 170 180 EAASYGVPAI AASMQVLDEG QKFDDPRDYQ RERFEAGIKV VNRIARNVLK RGMPENVDLL 190 200 210 220 230 240 NINIPFHAEE DTPIEITRLA RKIFKTDVEE RRDPRGRPYY WIAGDLIREE EEGTDVHAIM 250 260 QKGHVSITPI SLDSTARIDF SEIERYL

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM31087.1.
RefSeq	NP_633415.1. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PX31.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1479733.
GenomeReviews	Gene locus MM_1391 in contig AE008384_GR.
KEGG	mma:MM_1391.
NMPDR	fig|192952.1.peg.1391.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG600532.
OMA	SINVIYS.
PhylomeDB	Q8PX31.
ProtClustDB	PRK00346.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM1391-MONOMER.
Family and domain databases
HAMAP	MF_00060. SurE. [Tree]
InterPro	IPR002828. SurE-like_Pase/nucleotidase. [Graphical view]
Gene3D	G3DSA:3.40.1210.10. SurE-like_Pase/nucleotidase. 1 hit.
KO	K03787.
Pfam	PF01975. SurE. 1 hit. [Graphical view]
SUPFAM	SSF64167. SurE-like_Pase/nucleotidase. 1 hit.
TIGRFAMs	TIGR00087. SurE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SURE_METMA

Accession

Primary (citable) accession number: Q8PX31

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 19, 2002
Last modified:	December 14, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program