Q8PWY5 (IPYR_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	MM_1441

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00209.
Sequence similarities	Belongs to the PPase family.
Sequence caution	The sequence AAM31137.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137551
Sites
	Metal binding	56	1	Magnesium 1 By similarity
	Metal binding	61	1	Magnesium 1 By similarity
	Metal binding	61	1	Magnesium 2 By similarity
	Metal binding	93	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PWY5 [UniParc]. Last modified April 11, 2003. Version 2. Checksum: D0C8952C7427196C	FASTA	169	20,066
	10 20 30 40 50 60 MTERQVECVL IEIPQGSRNK YEYDKERKVI RFDRMLFSSI VYPCDYGFFP DTLALDGDPL 70 80 90 100 110 120 DAMVLMWEPT FPGCVIDVHP VAMLDMEDDK GRDEKILCVP QRDPLWNYIK TIEQVPPHLL 130 140 150 160 KEITHFFETY KNLERKDVVV YGWRDLETAR KVIQEAKDRY TEQKKLSNQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and analysis of proton-translocating pyrophosphatases in the methanogenic archaeon Methanosarcina mazei." Baeumer S., Lentes S., Gottschalk G., Deppenmeier U. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.
[2]	"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF312700 Genomic DNA. Translation: AAM22541.1. AE008384 Genomic DNA. Translation: AAM31137.1. Different initiation.
RefSeq	NP_633465.2. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PWY5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1479783.
GenomeReviews	Gene locus MM_1441 in contig AE008384_GR.
KEGG	mma:MM_1441.
NMPDR	fig|192952.1.peg.1441.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG529150.
OMA	SESAHEA.
PhylomeDB	Q8PWY5.
ProtClustDB	CLSK538096.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM1441-MONOMER.
Family and domain databases
HAMAP	MF_00209. Inorganic_PPase. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_METMA

Accession

Primary (citable) accession number: Q8PWY5
Secondary accession number(s): Q8NKW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	April 11, 2003
Last modified:	December 14, 2011
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program