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Q8PWY1

- PYLS_METMA

UniProt

Q8PWY1 - PYLS_METMA

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Protein

Pyrrolysine--tRNA ligase

Gene
pylS, MM_1445
Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG By similarity.UniRule annotation

Catalytic activityi

ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl).UniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA ligase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA aminoacylation for protein translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-1483-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrrolysine--tRNA ligase (EC:6.1.1.26)
Alternative name(s):
Pyrrolysine--tRNA(Pyl) ligase
Pyrrolysyl-tRNA synthetase
Short name:
PylRS
Gene namesi
Name:pylS
Ordered Locus Names:MM_1445
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000000595: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 454454Pyrrolysine--tRNA ligaseUniRule annotationPRO_0000260453Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi192952.MM_1445.

Structurei

Secondary structure

1
454
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi192 – 20110
Helixi204 – 2063
Beta strandi210 – 2134
Helixi216 – 23621
Helixi242 – 25615
Beta strandi260 – 2623
Beta strandi266 – 2694
Helixi270 – 2756
Helixi283 – 2875
Turni292 – 2943
Beta strandi295 – 2973
Beta strandi299 – 3013
Helixi302 – 31211
Turni313 – 3153
Beta strandi318 – 32912
Beta strandi335 – 3373
Beta strandi340 – 35112
Helixi356 – 37015
Beta strandi375 – 3817
Turni382 – 3843
Beta strandi385 – 3928
Beta strandi395 – 4039
Helixi406 – 4116
Beta strandi417 – 4237
Helixi424 – 4329
Helixi437 – 4404
Beta strandi444 – 4474
Beta strandi450 – 4523

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E3CX-ray2.65A185-454[»]
2Q7EX-ray1.80A185-454[»]
2Q7GX-ray1.90A185-454[»]
2Q7HX-ray2.10A185-454[»]
2ZCEX-ray1.80A185-454[»]
2ZIMX-ray2.10A185-454[»]
2ZINX-ray1.79A185-454[»]
2ZIOX-ray2.06A185-454[»]
3QTCX-ray1.75A185-454[»]
3VQVX-ray1.90A185-454[»]
3VQWX-ray2.40A185-454[»]
3VQXX-ray2.30A/B/C/D185-454[»]
4BW9X-ray2.35A185-454[»]
4BWAX-ray2.45A185-454[»]
4CH3X-ray2.28A185-454[»]
4CH4X-ray2.16A185-454[»]
4CH5X-ray2.20A185-454[»]
4CH6X-ray2.05A185-454[»]
4CS2X-ray1.90A188-454[»]
4CS3X-ray1.50A188-454[»]
4CS4X-ray1.35A188-454[»]
ProteinModelPortaliQ8PWY1.
SMRiQ8PWY1. Positions 188-454.

Miscellaneous databases

EvolutionaryTraceiQ8PWY1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG11047.
HOGENOMiHOG000115810.
KOiK11627.
OMAiNFCQMGS.

Family and domain databases

Gene3Di1.10.287.540. 1 hit.
HAMAPiMF_01573. Pyl_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR012739. Pyrrolysyl-tRNA_ligase.
IPR023877. Pyrrolysyl-tRNA_ligase_C.
IPR023878. Pyrrolysyl-tRNA_ligase_N.
IPR023218. UPF0291_struct_dom.
[Graphical view]
PfamiPF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02367. PylS_Cterm. 1 hit.
TIGR03912. PylS_Nterm. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8PWY1-1 [UniParc]FASTAAdd to Basket

« Hide

MDKKPLNTLI SATGLWMSRT GTIHKIKHHE VSRSKIYIEM ACGDHLVVNN    50
SRSSRTARAL RHHKYRKTCK RCRVSDEDLN KFLTKANEDQ TSVKVKVVSA 100
PTRTKKAMPK SVARAPKPLE NTEAAQAQPS GSKFSPAIPV STQESVSVPA 150
SVSTSISSIS TGATASALVK GNTNPITSMS APVQASAPAL TKSQTDRLEV 200
LLNPKDEISL NSGKPFRELE SELLSRRKKD LQQIYAEERE NYLGKLEREI 250
TRFFVDRGFL EIKSPILIPL EYIERMGIDN DTELSKQIFR VDKNFCLRPM 300
LAPNLYNYLR KLDRALPDPI KIFEIGPCYR KESDGKEHLE EFTMLNFCQM 350
GSGCTRENLE SIITDFLNHL GIDFKIVGDS CMVYGDTLDV MHGDLELSSA 400
VVGPIPLDRE WGIDKPWIGA GFGLERLLKV KHDFKNIKRA ARSESYYNGI 450
STNL 454
Length:454
Mass (Da):50,921
Last modified:October 1, 2002 - v1
Checksum:iD6BE5A812B5CA96E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008384 Genomic DNA. Translation: AAM31141.1.
RefSeqiNP_633469.1. NC_003901.1.
WP_011033391.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM31141; AAM31141; MM_1445.
GeneIDi1479787.
KEGGimma:MM_1445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008384 Genomic DNA. Translation: AAM31141.1 .
RefSeqi NP_633469.1. NC_003901.1.
WP_011033391.1. NC_003901.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2E3C X-ray 2.65 A 185-454 [» ]
2Q7E X-ray 1.80 A 185-454 [» ]
2Q7G X-ray 1.90 A 185-454 [» ]
2Q7H X-ray 2.10 A 185-454 [» ]
2ZCE X-ray 1.80 A 185-454 [» ]
2ZIM X-ray 2.10 A 185-454 [» ]
2ZIN X-ray 1.79 A 185-454 [» ]
2ZIO X-ray 2.06 A 185-454 [» ]
3QTC X-ray 1.75 A 185-454 [» ]
3VQV X-ray 1.90 A 185-454 [» ]
3VQW X-ray 2.40 A 185-454 [» ]
3VQX X-ray 2.30 A/B/C/D 185-454 [» ]
4BW9 X-ray 2.35 A 185-454 [» ]
4BWA X-ray 2.45 A 185-454 [» ]
4CH3 X-ray 2.28 A 185-454 [» ]
4CH4 X-ray 2.16 A 185-454 [» ]
4CH5 X-ray 2.20 A 185-454 [» ]
4CH6 X-ray 2.05 A 185-454 [» ]
4CS2 X-ray 1.90 A 188-454 [» ]
4CS3 X-ray 1.50 A 188-454 [» ]
4CS4 X-ray 1.35 A 188-454 [» ]
ProteinModelPortali Q8PWY1.
SMRi Q8PWY1. Positions 188-454.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 192952.MM_1445.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM31141 ; AAM31141 ; MM_1445 .
GeneIDi 1479787.
KEGGi mma:MM_1445.

Phylogenomic databases

eggNOGi NOG11047.
HOGENOMi HOG000115810.
KOi K11627.
OMAi NFCQMGS.

Enzyme and pathway databases

BioCyci MMAZ192952:GCK2-1483-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q8PWY1.

Family and domain databases

Gene3Di 1.10.287.540. 1 hit.
HAMAPi MF_01573. Pyl_tRNA_synth.
InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR012739. Pyrrolysyl-tRNA_ligase.
IPR023877. Pyrrolysyl-tRNA_ligase_C.
IPR023878. Pyrrolysyl-tRNA_ligase_N.
IPR023218. UPF0291_struct_dom.
[Graphical view ]
Pfami PF00587. tRNA-synt_2b. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02367. PylS_Cterm. 1 hit.
TIGR03912. PylS_Nterm. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
    Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R.
    , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Entry informationi

Entry nameiPYLS_METMA
AccessioniPrimary (citable) accession number: Q8PWY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 1, 2002
Last modified: September 3, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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