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Q8PWY1

- PYLS_METMA

UniProt

Q8PWY1 - PYLS_METMA

Protein

Pyrrolysine--tRNA ligase

Gene

pylS

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG.UniRule annotation

    Catalytic activityi

    ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl).UniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA ligase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA aminoacylation for protein translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMMAZ192952:GCK2-1483-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyrrolysine--tRNA ligaseUniRule annotation (EC:6.1.1.26UniRule annotation)
    Alternative name(s):
    Pyrrolysine--tRNA(Pyl) ligase
    Pyrrolysyl-tRNA synthetaseUniRule annotation
    Short name:
    PylRSUniRule annotation
    Gene namesi
    Name:pylSUniRule annotation
    Ordered Locus Names:MM_1445
    OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
    Taxonomic identifieri192952 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000000595: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 454454Pyrrolysine--tRNA ligasePRO_0000260453Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi192952.MM_1445.

    Structurei

    Secondary structure

    1
    454
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi192 – 20110
    Helixi204 – 2063
    Beta strandi210 – 2134
    Helixi216 – 23621
    Helixi242 – 25615
    Beta strandi260 – 2623
    Beta strandi266 – 2694
    Helixi270 – 2756
    Helixi283 – 2875
    Turni292 – 2943
    Beta strandi295 – 2973
    Beta strandi299 – 3013
    Helixi302 – 31211
    Turni313 – 3153
    Beta strandi318 – 32912
    Beta strandi335 – 3373
    Beta strandi340 – 35112
    Helixi356 – 37015
    Beta strandi375 – 3817
    Turni382 – 3843
    Beta strandi385 – 3928
    Beta strandi395 – 4039
    Helixi406 – 4116
    Beta strandi417 – 4237
    Helixi424 – 4329
    Helixi437 – 4404
    Beta strandi444 – 4474
    Beta strandi450 – 4523

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2E3CX-ray2.65A185-454[»]
    2Q7EX-ray1.80A185-454[»]
    2Q7GX-ray1.90A185-454[»]
    2Q7HX-ray2.10A185-454[»]
    2ZCEX-ray1.80A185-454[»]
    2ZIMX-ray2.10A185-454[»]
    2ZINX-ray1.79A185-454[»]
    2ZIOX-ray2.06A185-454[»]
    3QTCX-ray1.75A185-454[»]
    3VQVX-ray1.90A185-454[»]
    3VQWX-ray2.40A185-454[»]
    3VQXX-ray2.30A/B/C/D185-454[»]
    4BW9X-ray2.35A185-454[»]
    4BWAX-ray2.45A185-454[»]
    4CH3X-ray2.28A185-454[»]
    4CH4X-ray2.16A185-454[»]
    4CH5X-ray2.20A185-454[»]
    4CH6X-ray2.05A185-454[»]
    4CS2X-ray1.90A188-454[»]
    4CS3X-ray1.50A188-454[»]
    4CS4X-ray1.35A188-454[»]
    ProteinModelPortaliQ8PWY1.
    SMRiQ8PWY1. Positions 188-454.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8PWY1.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiNOG11047.
    HOGENOMiHOG000115810.
    KOiK11627.
    OMAiNFCQMGS.

    Family and domain databases

    Gene3Di1.10.287.540. 1 hit.
    HAMAPiMF_01573. Pyl_tRNA_synth.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR012739. Pyrrolysyl-tRNA_ligase.
    IPR023877. Pyrrolysyl-tRNA_ligase_C.
    IPR023878. Pyrrolysyl-tRNA_ligase_N.
    IPR023218. UPF0291_struct_dom.
    [Graphical view]
    PfamiPF00587. tRNA-synt_2b. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02367. PylS_Cterm. 1 hit.
    TIGR03912. PylS_Nterm. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8PWY1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKKPLNTLI SATGLWMSRT GTIHKIKHHE VSRSKIYIEM ACGDHLVVNN    50
    SRSSRTARAL RHHKYRKTCK RCRVSDEDLN KFLTKANEDQ TSVKVKVVSA 100
    PTRTKKAMPK SVARAPKPLE NTEAAQAQPS GSKFSPAIPV STQESVSVPA 150
    SVSTSISSIS TGATASALVK GNTNPITSMS APVQASAPAL TKSQTDRLEV 200
    LLNPKDEISL NSGKPFRELE SELLSRRKKD LQQIYAEERE NYLGKLEREI 250
    TRFFVDRGFL EIKSPILIPL EYIERMGIDN DTELSKQIFR VDKNFCLRPM 300
    LAPNLYNYLR KLDRALPDPI KIFEIGPCYR KESDGKEHLE EFTMLNFCQM 350
    GSGCTRENLE SIITDFLNHL GIDFKIVGDS CMVYGDTLDV MHGDLELSSA 400
    VVGPIPLDRE WGIDKPWIGA GFGLERLLKV KHDFKNIKRA ARSESYYNGI 450
    STNL 454
    Length:454
    Mass (Da):50,921
    Last modified:October 1, 2002 - v1
    Checksum:iD6BE5A812B5CA96E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008384 Genomic DNA. Translation: AAM31141.1.
    RefSeqiNP_633469.1. NC_003901.1.
    WP_011033391.1. NC_003901.1.

    Genome annotation databases

    EnsemblBacteriaiAAM31141; AAM31141; MM_1445.
    GeneIDi1479787.
    KEGGimma:MM_1445.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008384 Genomic DNA. Translation: AAM31141.1 .
    RefSeqi NP_633469.1. NC_003901.1.
    WP_011033391.1. NC_003901.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2E3C X-ray 2.65 A 185-454 [» ]
    2Q7E X-ray 1.80 A 185-454 [» ]
    2Q7G X-ray 1.90 A 185-454 [» ]
    2Q7H X-ray 2.10 A 185-454 [» ]
    2ZCE X-ray 1.80 A 185-454 [» ]
    2ZIM X-ray 2.10 A 185-454 [» ]
    2ZIN X-ray 1.79 A 185-454 [» ]
    2ZIO X-ray 2.06 A 185-454 [» ]
    3QTC X-ray 1.75 A 185-454 [» ]
    3VQV X-ray 1.90 A 185-454 [» ]
    3VQW X-ray 2.40 A 185-454 [» ]
    3VQX X-ray 2.30 A/B/C/D 185-454 [» ]
    4BW9 X-ray 2.35 A 185-454 [» ]
    4BWA X-ray 2.45 A 185-454 [» ]
    4CH3 X-ray 2.28 A 185-454 [» ]
    4CH4 X-ray 2.16 A 185-454 [» ]
    4CH5 X-ray 2.20 A 185-454 [» ]
    4CH6 X-ray 2.05 A 185-454 [» ]
    4CS2 X-ray 1.90 A 188-454 [» ]
    4CS3 X-ray 1.50 A 188-454 [» ]
    4CS4 X-ray 1.35 A 188-454 [» ]
    ProteinModelPortali Q8PWY1.
    SMRi Q8PWY1. Positions 188-454.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 192952.MM_1445.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM31141 ; AAM31141 ; MM_1445 .
    GeneIDi 1479787.
    KEGGi mma:MM_1445.

    Phylogenomic databases

    eggNOGi NOG11047.
    HOGENOMi HOG000115810.
    KOi K11627.
    OMAi NFCQMGS.

    Enzyme and pathway databases

    BioCyci MMAZ192952:GCK2-1483-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q8PWY1.

    Family and domain databases

    Gene3Di 1.10.287.540. 1 hit.
    HAMAPi MF_01573. Pyl_tRNA_synth.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR012739. Pyrrolysyl-tRNA_ligase.
    IPR023877. Pyrrolysyl-tRNA_ligase_C.
    IPR023878. Pyrrolysyl-tRNA_ligase_N.
    IPR023218. UPF0291_struct_dom.
    [Graphical view ]
    Pfami PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02367. PylS_Cterm. 1 hit.
    TIGR03912. PylS_Nterm. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
      Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R.
      , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
      J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

    Entry informationi

    Entry nameiPYLS_METMA
    AccessioniPrimary (citable) accession number: Q8PWY1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3