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Protein

Pyrrolysine--tRNA ligase

Gene

pylS

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG.UniRule annotation

Catalytic activityi

ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-1483-MONOMER.
BRENDAi6.1.1.26. 3270.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrrolysine--tRNA ligaseUniRule annotation (EC:6.1.1.26UniRule annotation)
Alternative name(s):
Pyrrolysine--tRNA(Pyl) ligase
Pyrrolysyl-tRNA synthetaseUniRule annotation
Short name:
PylRSUniRule annotation
Gene namesi
Name:pylSUniRule annotation
Ordered Locus Names:MM_1445
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000000595 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 454454Pyrrolysine--tRNA ligasePRO_0000260453Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi192952.MM_1445.

Structurei

Secondary structure

1
454
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi192 – 20110Combined sources
Helixi204 – 2063Combined sources
Beta strandi210 – 2145Combined sources
Helixi216 – 23621Combined sources
Helixi242 – 25615Combined sources
Beta strandi260 – 2623Combined sources
Beta strandi266 – 2694Combined sources
Helixi270 – 2756Combined sources
Helixi283 – 2875Combined sources
Turni292 – 2943Combined sources
Beta strandi295 – 2973Combined sources
Beta strandi299 – 3013Combined sources
Helixi302 – 31211Combined sources
Turni313 – 3153Combined sources
Beta strandi318 – 32912Combined sources
Beta strandi335 – 3373Combined sources
Beta strandi340 – 35112Combined sources
Helixi356 – 37015Combined sources
Beta strandi375 – 3817Combined sources
Turni382 – 3843Combined sources
Beta strandi385 – 3928Combined sources
Beta strandi395 – 4039Combined sources
Helixi406 – 4116Combined sources
Beta strandi417 – 4237Combined sources
Helixi424 – 4329Combined sources
Helixi437 – 4404Combined sources
Beta strandi444 – 4474Combined sources
Beta strandi450 – 4523Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E3CX-ray2.65A185-454[»]
2Q7EX-ray1.80A185-454[»]
2Q7GX-ray1.90A185-454[»]
2Q7HX-ray2.10A185-454[»]
2ZCEX-ray1.80A185-454[»]
2ZIMX-ray2.10A185-454[»]
2ZINX-ray1.79A185-454[»]
2ZIOX-ray2.06A185-454[»]
3QTCX-ray1.75A185-454[»]
3VQVX-ray1.90A185-454[»]
3VQWX-ray2.40A185-454[»]
3VQXX-ray2.30A/B/C/D185-454[»]
4BW9X-ray2.35A185-454[»]
4BWAX-ray2.45A185-454[»]
4CH3X-ray2.28A185-454[»]
4CH4X-ray2.16A185-454[»]
4CH5X-ray2.20A185-454[»]
4CH6X-ray2.05A185-454[»]
4CS2X-ray1.90A188-454[»]
4CS3X-ray1.50A188-454[»]
4CS4X-ray1.35A188-454[»]
4Q6GX-ray2.25A188-454[»]
4TQDX-ray2.14A185-454[»]
4TQFX-ray2.71A185-454[»]
ProteinModelPortaliQ8PWY1.
SMRiQ8PWY1. Positions 188-454.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8PWY1.

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiNOG11047.
HOGENOMiHOG000115810.
KOiK11627.
OMAiNFCQMGS.

Family and domain databases

Gene3Di1.10.287.540. 1 hit.
HAMAPiMF_01573. Pyl_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR012739. Pyrrolysyl-tRNA_ligase.
IPR023877. Pyrrolysyl-tRNA_ligase_C.
IPR023878. Pyrrolysyl-tRNA_ligase_N.
IPR023218. UPF0291_struct_dom.
[Graphical view]
PfamiPF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02367. PylS_Cterm. 1 hit.
TIGR03912. PylS_Nterm. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8PWY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKKPLNTLI SATGLWMSRT GTIHKIKHHE VSRSKIYIEM ACGDHLVVNN
60 70 80 90 100
SRSSRTARAL RHHKYRKTCK RCRVSDEDLN KFLTKANEDQ TSVKVKVVSA
110 120 130 140 150
PTRTKKAMPK SVARAPKPLE NTEAAQAQPS GSKFSPAIPV STQESVSVPA
160 170 180 190 200
SVSTSISSIS TGATASALVK GNTNPITSMS APVQASAPAL TKSQTDRLEV
210 220 230 240 250
LLNPKDEISL NSGKPFRELE SELLSRRKKD LQQIYAEERE NYLGKLEREI
260 270 280 290 300
TRFFVDRGFL EIKSPILIPL EYIERMGIDN DTELSKQIFR VDKNFCLRPM
310 320 330 340 350
LAPNLYNYLR KLDRALPDPI KIFEIGPCYR KESDGKEHLE EFTMLNFCQM
360 370 380 390 400
GSGCTRENLE SIITDFLNHL GIDFKIVGDS CMVYGDTLDV MHGDLELSSA
410 420 430 440 450
VVGPIPLDRE WGIDKPWIGA GFGLERLLKV KHDFKNIKRA ARSESYYNGI

STNL
Length:454
Mass (Da):50,921
Last modified:October 1, 2002 - v1
Checksum:iD6BE5A812B5CA96E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM31141.1.
RefSeqiNP_633469.1. NC_003901.1.
WP_011033391.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM31141; AAM31141; MM_1445.
GeneIDi1479787.
KEGGimma:MM_1445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM31141.1.
RefSeqiNP_633469.1. NC_003901.1.
WP_011033391.1. NC_003901.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E3CX-ray2.65A185-454[»]
2Q7EX-ray1.80A185-454[»]
2Q7GX-ray1.90A185-454[»]
2Q7HX-ray2.10A185-454[»]
2ZCEX-ray1.80A185-454[»]
2ZIMX-ray2.10A185-454[»]
2ZINX-ray1.79A185-454[»]
2ZIOX-ray2.06A185-454[»]
3QTCX-ray1.75A185-454[»]
3VQVX-ray1.90A185-454[»]
3VQWX-ray2.40A185-454[»]
3VQXX-ray2.30A/B/C/D185-454[»]
4BW9X-ray2.35A185-454[»]
4BWAX-ray2.45A185-454[»]
4CH3X-ray2.28A185-454[»]
4CH4X-ray2.16A185-454[»]
4CH5X-ray2.20A185-454[»]
4CH6X-ray2.05A185-454[»]
4CS2X-ray1.90A188-454[»]
4CS3X-ray1.50A188-454[»]
4CS4X-ray1.35A188-454[»]
4Q6GX-ray2.25A188-454[»]
4TQDX-ray2.14A185-454[»]
4TQFX-ray2.71A185-454[»]
ProteinModelPortaliQ8PWY1.
SMRiQ8PWY1. Positions 188-454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_1445.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM31141; AAM31141; MM_1445.
GeneIDi1479787.
KEGGimma:MM_1445.

Phylogenomic databases

eggNOGiNOG11047.
HOGENOMiHOG000115810.
KOiK11627.
OMAiNFCQMGS.

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-1483-MONOMER.
BRENDAi6.1.1.26. 3270.

Miscellaneous databases

EvolutionaryTraceiQ8PWY1.

Family and domain databases

Gene3Di1.10.287.540. 1 hit.
HAMAPiMF_01573. Pyl_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR012739. Pyrrolysyl-tRNA_ligase.
IPR023877. Pyrrolysyl-tRNA_ligase_C.
IPR023878. Pyrrolysyl-tRNA_ligase_N.
IPR023218. UPF0291_struct_dom.
[Graphical view]
PfamiPF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02367. PylS_Cterm. 1 hit.
TIGR03912. PylS_Nterm. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
    Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R.
    , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Entry informationi

Entry nameiPYLS_METMA
AccessioniPrimary (citable) accession number: Q8PWY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 1, 2002
Last modified: April 29, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.