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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. tryptophan-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tryptophanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-1509-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:trpS
Ordered Locus Names:MM_1471
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000000595 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Tryptophan--tRNA ligasePRO_0000136725Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi192952.MM_1471.

Structurei

3D structure databases

ProteinModelPortaliQ8PWV5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni295 – 34955InsertAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi74 – 829"HIGH" region
Motifi375 – 3795"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000224743.
KOiK01867.
OMAiSIYHRFM.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
HAMAPiMF_00140_A. Trp_tRNA_synth_A.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR020653. Tryptophan-tRNA-ligase_arc.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 2 hits.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.

Sequencei

Sequence statusi: Complete.

Q8PWV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNKLDPWSS SNITDYSKLF EEFGISPFEN ILPEIPSPHK YMRRRIIFGH
60 70 80 90 100
RDYEQIAEAM RTGAPFSVMD GFMPSGKVHL GHKMVMDQIV WHQEKGASAF
110 120 130 140 150
VGIADREAFS VRGFSWQKCR EIGVEEYILS LIALGFKPEG LIYFQSGCGS
160 170 180 190 200
VKDLAFELGA KVNFSELSAI YGFSGETSLS HMLSVATQAA DILQPQLEEF
210 220 230 240 250
GGSKPVVVPV GPDQDPHLRL TRGLAGKMNM FRVEERETAE GGKYLSVRGK
260 270 280 290 300
GAPREALQEL KKRIPGKVKL YEEHIDVLQT PDYAFLERFV LQINQPERRN
310 320 330 340 350
YFMTQIQEIA TFANAAKPPE FSEYEKYFVF KRVWKTTGKI LEEVVAEVAA
360 370 380 390 400
EFDGYAFIPP ASTYHRFMSG LQGGKMSSSI PDSHIALTDD PKEGAKKVKR
410 420 430 440 450
AKTGGCITLE EQKKLGGKPD ECSVFELMLF HLIEDDEELL EIREECIYGT
460 470 480 490
RMCGSCKQLA AEKMQEFLKD HQEKRELARE QLDEYRIIYK K
Length:491
Mass (Da):55,728
Last modified:November 8, 2002 - v2
Checksum:iD7ADE51A4F4E9D3C
GO

Sequence cautioni

The sequence AAM31167.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM31167.1. Different initiation.
RefSeqiNP_633495.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM31167; AAM31167; MM_1471.
GeneIDi1479813.
KEGGimma:MM_1471.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM31167.1. Different initiation.
RefSeqiNP_633495.1. NC_003901.1.

3D structure databases

ProteinModelPortaliQ8PWV5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_1471.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM31167; AAM31167; MM_1471.
GeneIDi1479813.
KEGGimma:MM_1471.

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000224743.
KOiK01867.
OMAiSIYHRFM.

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-1509-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
HAMAPiMF_00140_A. Trp_tRNA_synth_A.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR020653. Tryptophan-tRNA-ligase_arc.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 2 hits.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
ProtoNetiSearch...

Publicationsi

  1. "The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
    Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R.
    , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Entry informationi

Entry nameiSYW_METMA
AccessioniPrimary (citable) accession number: Q8PWV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: January 7, 2015
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.