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Q8PWV5 (SYW_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:MM_1471
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140_A

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140_A.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAM31167.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Tryptophan--tRNA ligase HAMAP-Rule MF_00140_A
PRO_0000136725

Regions

Region295 – 34955Insert HAMAP-Rule MF_00140_A
Motif74 – 829"HIGH" region HAMAP-Rule MF_00140_A
Motif375 – 3795"KMSKS" region HAMAP-Rule MF_00140_A

Sequences

Sequence LengthMass (Da)Tools
Q8PWV5 [UniParc].

Last modified November 8, 2002. Version 2.
Checksum: D7ADE51A4F4E9D3C

FASTA49155,728
        10         20         30         40         50         60 
MDNKLDPWSS SNITDYSKLF EEFGISPFEN ILPEIPSPHK YMRRRIIFGH RDYEQIAEAM 

        70         80         90        100        110        120 
RTGAPFSVMD GFMPSGKVHL GHKMVMDQIV WHQEKGASAF VGIADREAFS VRGFSWQKCR 

       130        140        150        160        170        180 
EIGVEEYILS LIALGFKPEG LIYFQSGCGS VKDLAFELGA KVNFSELSAI YGFSGETSLS 

       190        200        210        220        230        240 
HMLSVATQAA DILQPQLEEF GGSKPVVVPV GPDQDPHLRL TRGLAGKMNM FRVEERETAE 

       250        260        270        280        290        300 
GGKYLSVRGK GAPREALQEL KKRIPGKVKL YEEHIDVLQT PDYAFLERFV LQINQPERRN 

       310        320        330        340        350        360 
YFMTQIQEIA TFANAAKPPE FSEYEKYFVF KRVWKTTGKI LEEVVAEVAA EFDGYAFIPP 

       370        380        390        400        410        420 
ASTYHRFMSG LQGGKMSSSI PDSHIALTDD PKEGAKKVKR AKTGGCITLE EQKKLGGKPD 

       430        440        450        460        470        480 
ECSVFELMLF HLIEDDEELL EIREECIYGT RMCGSCKQLA AEKMQEFLKD HQEKRELARE 

       490 
QLDEYRIIYK K 

« Hide

References

[1]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008384 Genomic DNA. Translation: AAM31167.1. Different initiation.
RefSeqNP_633495.1. NC_003901.1.

3D structure databases

ProteinModelPortalQ8PWV5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING192952.MM_1471.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM31167; AAM31167; MM_1471.
GeneID1479813.
KEGGmma:MM_1471.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000224743.
KOK01867.
OMASIYHRFM.

Enzyme and pathway databases

BioCycMMAZ192952:GCK2-1509-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
HAMAPMF_00140_A. Trp_tRNA_synth_A.
InterProIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR020653. Tryptophan-tRNA-ligase_arc.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 2 hits.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
ProtoNetSearch...

Entry information

Entry nameSYW_METMA
AccessionPrimary (citable) accession number: Q8PWV5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: May 14, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries