Q8PWV5 (SYW_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	trpS
Ordered Locus Names:	MM_1471

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina mazei

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140_A
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence AAM31167.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Tryptophan--tRNA ligase HAMAP-Rule MF_00140_A		PRO_0000136725
Regions
	Region	295 – 349	55	Insert HAMAP-Rule MF_00140_A
	Motif	74 – 82	9	"HIGH" region HAMAP-Rule MF_00140_A
	Motif	375 – 379	5	"KMSKS" region HAMAP-Rule MF_00140_A

Sequences

	Sequence		Length	Mass (Da)
	Q8PWV5 [UniParc]. Last modified November 8, 2002. Version 2. Checksum: D7ADE51A4F4E9D3C	FASTA	491	55,728
	10 20 30 40 50 60 MDNKLDPWSS SNITDYSKLF EEFGISPFEN ILPEIPSPHK YMRRRIIFGH RDYEQIAEAM 70 80 90 100 110 120 RTGAPFSVMD GFMPSGKVHL GHKMVMDQIV WHQEKGASAF VGIADREAFS VRGFSWQKCR 130 140 150 160 170 180 EIGVEEYILS LIALGFKPEG LIYFQSGCGS VKDLAFELGA KVNFSELSAI YGFSGETSLS 190 200 210 220 230 240 HMLSVATQAA DILQPQLEEF GGSKPVVVPV GPDQDPHLRL TRGLAGKMNM FRVEERETAE 250 260 270 280 290 300 GGKYLSVRGK GAPREALQEL KKRIPGKVKL YEEHIDVLQT PDYAFLERFV LQINQPERRN 310 320 330 340 350 360 YFMTQIQEIA TFANAAKPPE FSEYEKYFVF KRVWKTTGKI LEEVVAEVAA EFDGYAFIPP 370 380 390 400 410 420 ASTYHRFMSG LQGGKMSSSI PDSHIALTDD PKEGAKKVKR AKTGGCITLE EQKKLGGKPD 430 440 450 460 470 480 ECSVFELMLF HLIEDDEELL EIREECIYGT RMCGSCKQLA AEKMQEFLKD HQEKRELARE 490 QLDEYRIIYK K

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM31167.1. Different initiation.
RefSeq	NP_633495.1. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PWV5.
ModBase	Search...
Protein-protein interaction databases
STRING	192952.MM_1471.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM31167; AAM31167; MM_1471.
GeneID	1479813.
KEGG	mma:MM_1471.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0180.
HOGENOM	HOG000224743.
KO	K01867.
OMA	SIYHRFM.
ProtClustDB	PRK12285.
Family and domain databases
Gene3D	3.40.50.620. 2 hits.
HAMAP	MF_00140_A. Trp_tRNA_synth_A. Divergent sequence.
InterPro	IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR020653. Tryptophan-tRNA-ligase_arc. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 2 hits. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW_METMA

Accession

Primary (citable) accession number: Q8PWV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	November 8, 2002
Last modified:	May 1, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program