Q8PWU0 (SYA_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	MM_1486

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	926 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 926	926	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000075266
Sites
	Metal binding	611	1	Zinc By similarity
	Metal binding	615	1	Zinc By similarity
	Metal binding	714	1	Zinc By similarity
	Metal binding	718	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PWU0 [UniParc]. Last modified October 25, 2002. Version 1. Checksum: 065D616BFFC6B5FF	FASTA	926	103,730
	10 20 30 40 50 60 MLEDEYQLDF FKNNGFVRKQ CQKCGTFFWT RDPERNTCGD APCDPYSFIG SPVFSREFNI 70 80 90 100 110 120 SEMREYYLSF FEARGHTRIN RYPVVARWRD DIYLTIASIA DFQPFVTSGQ VPPPANPLTI 130 140 150 160 170 180 SQPCIRLNDL DSVGRSGRHL TNFEMMAHHA FNRRDNEIYW KEHTLELCDE LLNSLKVNPL 190 200 210 220 230 240 AVTYKEEPWA GGGNAGPCVE VIVHGLELAT LVFMDLKTDK KGDIEIKGET YSKMDNYIVD 250 260 270 280 290 300 TGYGLERFVW ASRGSPTIYD ALFPGIVNEL MGLAGIEHEL NDSEYANILA QNARLAGFMD 310 320 330 340 350 360 VSEKANLMEL RKKVASSIGM TVDKLSAIME PVEKVYAITD HTRCLTFMLG DGIIPSNVKA 370 380 390 400 410 420 GYLARLVLRR TLRMMKDLDI RIPLSEIVDM HIKNMPEYPG FRENFPVIQD ILESEEEKFN 430 440 450 460 470 480 TTMERGRRII QKSASHFKKT GEKIPLSQLT ELYDSHGIPP EMAKEVAAEI GVGVEFPDNF 490 500 510 520 530 540 YSIIGELHNK AEEKEEEVNP YAERLKHLPK TKRRFYDEPT RLEFEAVVLD VFDNHVVLDN 550 560 570 580 590 600 TFFYAEGGGQ PADIGTIVAE DTVYRVVDVQ VYEGVILHTI ENPGKELAIT KGELITGKVD 610 620 630 640 650 660 EKRRMTLARH HTATHIVNDA ARKVLGKHIW QAGAQKFEDH SRLDLSHYKH ISPEEIRQIE 670 680 690 700 710 720 LLANRTVMEN KRVVTEWMPR TEAEQVYGFG LYQGGVPPGE KIRIVKVGDD VEACGGTHCT 730 740 750 760 770 780 STGIIGPIKI LKTERIQDGV ERIEFAAGTA AVRAMQKLES LLVDSSKTLS VPPEHLPVSV 790 800 810 820 830 840 DRFFGEWKDL KKENERLKEE LARSRVYRML GDASEVSGLK VITEQVSGAD SLELQKIATE 850 860 870 880 890 900 LLKTENVVAL LASDFEGVKI VASAGEKAMK CGVNAGNLVR EMSKIVGGGG GGKPALAMGG 910 920 GTDPTRIQDA LTRGLELVKT AACKEA

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM31182.1.
RefSeq	NP_633510.1. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PWU0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1479828.
GenomeReviews	Gene locus MM_1486 in contig AE008384_GR.
KEGG	mma:MM_1486.
NMPDR	fig|192952.1.peg.1486.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG392147.
OMA	MFTNSGM.
PhylomeDB	Q8PWU0.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM1486-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_METMA

Accession

Primary (citable) accession number: Q8PWU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 25, 2002
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program