Q8PWS3 (HIS1_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP phosphoribosyltransferase
Short name=ATP-PRT
Short name=ATP-PRTase
EC=2.4.2.17

Gene names

Name:	hisG
Ordered Locus Names:	MM_1503

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity By similarity. HAMAP MF_00079
Catalytic activity	1-(5-phospho-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00079
Cofactor	Magnesium By similarity. HAMAP MF_00079
Enzyme regulation	Feedback inhibited by histidine By similarity. HAMAP MF_00079
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9. HAMAP MF_00079
Subcellular location	Cytoplasm By similarity HAMAP MF_00079.
Sequence similarities	Belongs to the ATP phosphoribosyltransferase family. Long subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	ATP phosphoribosyltransferase HAMAP MF_00079		PRO_0000151884

Sequences

	Sequence		Length	Mass (Da)
	Q8PWS3 [UniParc]. Last modified October 10, 2002. Version 1. Checksum: B65D7E38C7320F00	FASTA	289	31,527
	10 20 30 40 50 60 MIRIAIPNKG RLYEPTISIF KDAGLPISGG AESRKLFAKT TDPDIHILFA RAADIPEYVQ 70 80 90 100 110 120 DGAADVGITG MDLITERGAN VEALLDLKFG RANLVLAVPE DSDFEKAQDL EGKKVATEFP 130 140 150 160 170 180 EITRRYFEKL GVNVNVIKVS GACEMTPHVG IADAIVDISS SGTTLLINHL KAIDMAFSST 190 200 210 220 230 240 VYLIANKESL RTKEKILDIK TAFESVLNAK KKRYLMMNVP ESSLKAVKEV LPGMSGPTVM 250 260 270 280 KVESSKFSEE SILAVHAVVD ADLIFTIVNR LKKVGARDVL VVPIERIMP

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM31199.1.
RefSeq	NP_633527.1. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PWS3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1479845.
GenomeReviews	Gene locus MM_1503 in contig AE008384_GR.
KEGG	mma:MM_1503.
NMPDR	fig|192952.1.peg.1503.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391868.
OMA	IMLHAPS.
PhylomeDB	Q8PWS3.
ProtClustDB	PRK00489.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM1503-MONOMER.
Family and domain databases
HAMAP	MF_00079. HisG_Long. [Tree]
InterPro	IPR013820. ATP_PRibTrfase_cat. IPR018198. ATP_PRibTrfase_CS. IPR001348. ATP_PRibTrfase_HisG. IPR020621. ATP_PRibTrfase_HisG_long. IPR013115. HisG_C. IPR011322. N-reg_PII-like_a/b. IPR015867. N-reg_PII/ATP_PRibTrfase_C. [Graphical view]
Gene3D	G3DSA:3.30.70.120. PII_glnB. 1 hit.
KO	K00765.
PANTHER	PTHR21403. ATP_phspho_trans. 1 hit.
Pfam	PF01634. HisG. 1 hit. PF08029. HisG_C. 1 hit. [Graphical view]
SUPFAM	SSF54913. N-reg_PII-like_a/b. 1 hit.
TIGRFAMs	TIGR00070. HisG. 1 hit. TIGR03455. HisG_C-term. 1 hit.
PROSITE	PS01316. ATP_P_PHORIBOSYLTR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HIS1_METMA

Accession

Primary (citable) accession number: Q8PWS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	October 10, 2002
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program