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Q8PWG1 (DUT_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable deoxyuridine 5'-triphosphate nucleotidohydrolase

Short name=dUTPase
EC=3.6.1.23
Alternative name(s):
dUTP pyrophosphatase
Gene names
Name:dut
Ordered Locus Names:MM_1628
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length171 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00635

Catalytic activity

dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00635

Pathway

Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00635

Sequence similarities

Belongs to the dCTP deaminase family. Archaeal dUTPase subfamily.

Ontologies

Keywords
   Biological processNucleotide metabolism
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdUMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

dUTP metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functiondUTP diphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 171171Probable deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00635
PRO_0000153640

Sequences

Sequence LengthMass (Da)Tools
Q8PWG1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: A6E96FE90FF20A67

FASTA17119,028
        10         20         30         40         50         60 
MALLSSNELR KLIKANPPLL ENAVDTETQI QPNGLELTLK EIKTIEGAGA VDFDNSERKV 

        70         80         90        100        110        120 
PDAKPLEFGK DGWIHLPEGI YKVIFNEIVN IPMNLAAIAK PRSSLIRCGA TLETAVWDAG 

       130        140        150        160        170 
YRGRSESMLV VYNPAGFKLK KNARIMQLLF YTLNTEVEEG YSGVYQNENT N 

« Hide

References

[1]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008384 Genomic DNA. Translation: AAM31324.1.
RefSeqNP_633652.1. NC_003901.1.

3D structure databases

ProteinModelPortalQ8PWG1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING192952.MM_1628.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM31324; AAM31324; MM_1628.
GeneID1479970.
KEGGmma:MM_1628.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0717.
HOGENOMHOG000228599.
KOK01520.
OMAWDAGYEG.

Enzyme and pathway databases

BioCycMMAZ192952:GCK2-1669-MONOMER.
UniPathwayUPA00610; UER00666.

Family and domain databases

Gene3D2.70.40.10. 1 hit.
HAMAPMF_00635. dUTPase_arch.
InterProIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR023537. dUTPase_archaeal.
[Graphical view]
PfamPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMSSF51283. SSF51283. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDUT_METMA
AccessionPrimary (citable) accession number: Q8PWG1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: October 1, 2002
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways