Q8PWG1 (DUT_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	MM_1628

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina mazei

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00635
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00635
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00635
Sequence similarities	Belongs to the dCTP deaminase family. Archaeal dUTPase subfamily.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Probable deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00635		PRO_0000153640

Sequences

	Sequence		Length	Mass (Da)
	Q8PWG1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: A6E96FE90FF20A67	FASTA	171	19,028
	10 20 30 40 50 60 MALLSSNELR KLIKANPPLL ENAVDTETQI QPNGLELTLK EIKTIEGAGA VDFDNSERKV 70 80 90 100 110 120 PDAKPLEFGK DGWIHLPEGI YKVIFNEIVN IPMNLAAIAK PRSSLIRCGA TLETAVWDAG 130 140 150 160 170 YRGRSESMLV VYNPAGFKLK KNARIMQLLF YTLNTEVEEG YSGVYQNENT N

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM31324.1.
RefSeq	NP_633652.1. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PWG1.
ModBase	Search...
Protein-protein interaction databases
STRING	192952.MM_1628.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM31324; AAM31324; MM_1628.
GeneID	1479970.
KEGG	mma:MM_1628.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0717.
HOGENOM	HOG000228599.
KO	K01520.
OMA	WDAGYEG.
ProtClustDB	PRK02253.
Enzyme and pathway databases
UniPathway	UPA00610; UER00666.
Family and domain databases
HAMAP	MF_00635. dUTPase_arch.
InterPro	IPR008180. dUTP_pyroPase. IPR023537. dUTP_pyroPase_arch. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUT_METMA

Accession

Primary (citable) accession number: Q8PWG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program