ID   SYK1_METMA              Reviewed;         537 AA.
AC   Q8PWA0;
DT   25-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2002, sequence version 1.
DT   27-MAR-2024, entry version 123.
DE   RecName: Full=Lysine--tRNA ligase 1 {ECO:0000255|HAMAP-Rule:MF_00177};
DE            EC=6.1.1.6 {ECO:0000255|HAMAP-Rule:MF_00177};
DE   AltName: Full=Lysyl-tRNA synthetase 1 {ECO:0000255|HAMAP-Rule:MF_00177};
DE            Short=LysRS 1 {ECO:0000255|HAMAP-Rule:MF_00177};
GN   Name=lysS1 {ECO:0000255|HAMAP-Rule:MF_00177}; Synonyms=lysS;
GN   OrderedLocusNames=MM_1696;
OS   Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS   11833 / OCM 88) (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=192952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT   between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC         tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC         COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00177};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00177}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00177}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM31392.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE008384; AAM31392.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_015412023.1; NC_003901.1.
DR   AlphaFoldDB; Q8PWA0; -.
DR   SMR; Q8PWA0; -.
DR   GeneID; 66136815; -.
DR   KEGG; mma:MM_1696; -.
DR   PATRIC; fig|192952.21.peg.1970; -.
DR   eggNOG; arCOG00485; Archaea.
DR   HOGENOM; CLU_025562_1_0_2; -.
DR   Proteomes; UP000000595; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   CDD; cd00674; LysRS_core_class_I; 1.
DR   Gene3D; 1.10.10.350; -; 1.
DR   Gene3D; 1.10.10.770; -; 1.
DR   Gene3D; 3.40.50.620; HUPs; 2.
DR   Gene3D; 6.10.20.10; Lysine tRNA ligase, stem contact fold domain; 1.
DR   HAMAP; MF_00177; Lys_tRNA_synth_class1; 1.
DR   InterPro; IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR008925; aa_tRNA-synth_I_cd-bd_sf.
DR   InterPro; IPR002904; Lys-tRNA-ligase.
DR   InterPro; IPR042078; Lys-tRNA-ligase_SC_fold.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   NCBIfam; TIGR00467; lysS_arch; 1.
DR   PANTHER; PTHR37940; LYSINE--TRNA LIGASE; 1.
DR   PANTHER; PTHR37940:SF1; LYSINE--TRNA LIGASE; 1.
DR   Pfam; PF01921; tRNA-synt_1f; 1.
DR   SUPFAM; SSF48163; An anticodon-binding domain of class I aminoacyl-tRNA synthetases; 1.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..537
FT                   /note="Lysine--tRNA ligase 1"
FT                   /id="PRO_0000152755"
FT   MOTIF           30..38
FT                   /note="'HIGH' region"
FT   MOTIF           276..280
FT                   /note="'KMSKS' region"
SQ   SEQUENCE   537 AA;  60333 MW;  B8666F036BA375DD CRC64;
     MADTIHWADV IAEDVLNKSG KHLVATGITP SGHIHIGNMR EVVTADAAYR ALLDRGADAR
     FIYIADNFDP LRKVYPFLPE SYAEHVGKPI SEVPCPCGEC ANYSEHFLKP FLEALRRLGI
     NPEVYRADEM YRAGMYTEAI KTALVKRNEI AKILEEVSGK TVAEEWSPFN PRCNECGKIT
     TTKVTGFDPD AETVDYVCAC GHSGTVPMAG GGKLTWRVDW PARWAVLGVT VEPFGKDHAS
     RGGSYDTGKR IVREIYGHEP PFPIVYEWIM LGKQGAMSSS TGVVVSISDM LEVVPPEVLR
     YLIIRTKPEK HIQFDPAQPL LTIVDEYERL REKFRDNDPS MGEFEKRIYE LSRATGICHP
     EIPFKQMVTI FQVARGNFEQ ILQIVKRSGF STEDEKCIRE LVDNVSKWLE LYAPPFAKFS
     VKENVPVQVA TLSELQKAFL GAFADLIESK GKISGEEYHM LVYSAKEKGS ELNRLIAEKL
     NVPAPQVDPK DLFKAIYTSI LGQSSGPKAG WFLSSFEEDF LINRFREAST YSPEKRE
//