Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8PWA0 (SYK1_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysine--tRNA ligase 1

EC=6.1.1.6
Alternative name(s):
Lysyl-tRNA synthetase 1
Short name=LysRS 1
Gene names
Name:lysS1
Synonyms:lysS
Ordered Locus Names:MM_1696
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length537 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00177

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00177.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAM31392.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlysyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lysine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 537537Lysine--tRNA ligase 1 HAMAP-Rule MF_00177
PRO_0000152755

Regions

Motif30 – 389"HIGH" region HAMAP-Rule MF_00177
Motif276 – 2805"KMSKS" region HAMAP-Rule MF_00177

Sequences

Sequence LengthMass (Da)Tools
Q8PWA0 [UniParc].

Last modified October 25, 2002. Version 1.
Checksum: B8666F036BA375DD

FASTA53760,333
        10         20         30         40         50         60 
MADTIHWADV IAEDVLNKSG KHLVATGITP SGHIHIGNMR EVVTADAAYR ALLDRGADAR 

        70         80         90        100        110        120 
FIYIADNFDP LRKVYPFLPE SYAEHVGKPI SEVPCPCGEC ANYSEHFLKP FLEALRRLGI 

       130        140        150        160        170        180 
NPEVYRADEM YRAGMYTEAI KTALVKRNEI AKILEEVSGK TVAEEWSPFN PRCNECGKIT 

       190        200        210        220        230        240 
TTKVTGFDPD AETVDYVCAC GHSGTVPMAG GGKLTWRVDW PARWAVLGVT VEPFGKDHAS 

       250        260        270        280        290        300 
RGGSYDTGKR IVREIYGHEP PFPIVYEWIM LGKQGAMSSS TGVVVSISDM LEVVPPEVLR 

       310        320        330        340        350        360 
YLIIRTKPEK HIQFDPAQPL LTIVDEYERL REKFRDNDPS MGEFEKRIYE LSRATGICHP 

       370        380        390        400        410        420 
EIPFKQMVTI FQVARGNFEQ ILQIVKRSGF STEDEKCIRE LVDNVSKWLE LYAPPFAKFS 

       430        440        450        460        470        480 
VKENVPVQVA TLSELQKAFL GAFADLIESK GKISGEEYHM LVYSAKEKGS ELNRLIAEKL 

       490        500        510        520        530 
NVPAPQVDPK DLFKAIYTSI LGQSSGPKAG WFLSSFEEDF LINRFREAST YSPEKRE 

« Hide

References

[1]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008384 Genomic DNA. Translation: AAM31392.1. Different initiation.
RefSeqNP_633720.1. NC_003901.1.

3D structure databases

ProteinModelPortalQ8PWA0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING192952.MM_1696.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM31392; AAM31392; MM_1696.
GeneID1480038.
KEGGmma:MM_1696.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1384.
HOGENOMHOG000226938.
KOK04566.
OMAWPARWSI.

Enzyme and pathway databases

BioCycMMAZ192952:GCK2-1739-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00177. Lys_tRNA_synth_class1.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR002904. Lys-tRNA-ligase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF01921. tRNA-synt_1f. 1 hit.
[Graphical view]
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00467. lysS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYK1_METMA
AccessionPrimary (citable) accession number: Q8PWA0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: October 25, 2002
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries