Q8PWA0 (SYK1_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysine--tRNA ligase 1
EC=6.1.1.6

Alternative name(s):
Lysyl-tRNA synthetase 1
Short name=LysRS 1

Gene names

Name:	lysS1
Synonyms:	lysS
Ordered Locus Names:	MM_1696

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina mazei

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00177
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00177.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence AAM31392.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 537	537	Lysine--tRNA ligase 1 HAMAP-Rule MF_00177		PRO_0000152755
Regions
	Motif	30 – 38	9	"HIGH" region HAMAP-Rule MF_00177
	Motif	276 – 280	5	"KMSKS" region HAMAP-Rule MF_00177

Sequences

	Sequence		Length	Mass (Da)
	Q8PWA0 [UniParc]. Last modified October 25, 2002. Version 1. Checksum: B8666F036BA375DD	FASTA	537	60,333
	10 20 30 40 50 60 MADTIHWADV IAEDVLNKSG KHLVATGITP SGHIHIGNMR EVVTADAAYR ALLDRGADAR 70 80 90 100 110 120 FIYIADNFDP LRKVYPFLPE SYAEHVGKPI SEVPCPCGEC ANYSEHFLKP FLEALRRLGI 130 140 150 160 170 180 NPEVYRADEM YRAGMYTEAI KTALVKRNEI AKILEEVSGK TVAEEWSPFN PRCNECGKIT 190 200 210 220 230 240 TTKVTGFDPD AETVDYVCAC GHSGTVPMAG GGKLTWRVDW PARWAVLGVT VEPFGKDHAS 250 260 270 280 290 300 RGGSYDTGKR IVREIYGHEP PFPIVYEWIM LGKQGAMSSS TGVVVSISDM LEVVPPEVLR 310 320 330 340 350 360 YLIIRTKPEK HIQFDPAQPL LTIVDEYERL REKFRDNDPS MGEFEKRIYE LSRATGICHP 370 380 390 400 410 420 EIPFKQMVTI FQVARGNFEQ ILQIVKRSGF STEDEKCIRE LVDNVSKWLE LYAPPFAKFS 430 440 450 460 470 480 VKENVPVQVA TLSELQKAFL GAFADLIESK GKISGEEYHM LVYSAKEKGS ELNRLIAEKL 490 500 510 520 530 NVPAPQVDPK DLFKAIYTSI LGQSSGPKAG WFLSSFEEDF LINRFREAST YSPEKRE

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM31392.1. Different initiation.
RefSeq	NP_633720.1. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PWA0.
ModBase	Search...
Protein-protein interaction databases
STRING	192952.MM_1696.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM31392; AAM31392; MM_1696.
GeneID	1480038.
KEGG	mma:MM_1696.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1384.
HOGENOM	HOG000226938.
KO	K04566.
OMA	FIFSWDD.
ProtClustDB	PRK00750.
Enzyme and pathway databases
BioCyc	MMAZ192952:GCK2-1739-MONOMER.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00177. Lys_tRNA_synth_class1.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR002904. Lys-tRNA-ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Pfam	PF01921. tRNA-synt_1f. 1 hit. [Graphical view]
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00467. lysS_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK1_METMA

Accession

Primary (citable) accession number: Q8PWA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 25, 2002
Last modified:	May 29, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program