Q8PW90 (PIMT_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-L-isoaspartate O-methyltransferase
EC=2.1.1.77

Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Short name=PIMT

Gene names

Name:	pcm
Ordered Locus Names:	MM_1706

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity. HAMAP MF_00090
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP MF_00090
Subcellular location	Cytoplasm By similarity HAMAP MF_00090.
Sequence similarities	Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Protein-L-isoaspartate O-methyltransferase HAMAP MF_00090		PRO_0000111918
Sites
	Active site	87	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PW90 [UniParc]. Last modified October 10, 2002. Version 1. Checksum: DF83E0D0591A1B1C	FASTA	243	27,120
	10 20 30 40 50 60 MPERRGIVLV NAVSEKDLGE DKRKREKEKE CEKLRKLLVE GLEGFVIDEK VLQAMLRVPR 70 80 90 100 110 120 HRFVPEYEQR AAYVDMPLEI GHGQTISAPH MVAMMCEILE LAEGHKVLEI GAGSGYNAAV 130 140 150 160 170 180 MSELVGKTGH IYTVERVEPL ANFAKKNLKE AGYKNVTVLL ENGSMGYPGY APYDRIAVTC 190 200 210 220 230 240 AAPNIPETLL EQLKPGGIMV IPVGSYSQEL IRVKKDSTGK IYRKKKGDVI FVPMIGKHGF RRI

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM31402.1.
RefSeq	NP_633730.1. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PW90.
SMR	Q8PW90. Positions 38-241.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1480048.
GenomeReviews	Gene locus MM_1706 in contig AE008384_GR.
KEGG	mma:MM_1706.
NMPDR	fig|192952.1.peg.1706.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG699907.
OMA	MGAKEDL.
PhylomeDB	Q8PW90.
ProtClustDB	PRK13942.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM1706-MONOMER.
Family and domain databases
HAMAP	MF_00090. PIMT. [Tree]
InterPro	IPR000682. PCMT. [Graphical view]
KO	K00573.
PANTHER	PTHR11579. PCMT. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. Pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_METMA

Accession

Primary (citable) accession number: Q8PW90

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	October 10, 2002
Last modified:	December 14, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program