Reviewed, UniProtKB/Swiss-Prot Q8PVW4 (HDRE_METMA)

Names and origin

Protein names

Recommended name:
    CoB--CoM heterodisulfide reductase 2 subunit E
    EC=1.8.98.1

Gene names

Name:	hdrE
Ordered Locus Names:	MM_1843

Organism

Methanosarcina mazei (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

2209 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B). HdrE may be responsible for anchoring the complex to the membrane. Ref.2
Catalytic activity	Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine.
Pathway	Cofactor metabolism; coenzyme B/coenzyme M regeneration; coenzyme B and coenzyme M from CoB-CoM heterodisulfide: step 1/1.
Subunit structure	The heterodisulfide reductase 2 is composed of two subunits; hdrD and hdrE By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Miscellaneous	Methanophenazine seems to mediate electron transfer from a F420-non-reducing hydrogenase to the heterodisulfide reductase 2.
Sequence similarities	Belongs to the hdrE family.

Ontologies

Keywords
Biological process	Methanogenesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	CoB--CoM heterodisulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	CoB--CoM heterodisulfide reductase 2 subunit E		PRO_0000150084
Regions
	Transmembrane	15 – 35	21	Potential
	Transmembrane	104 – 124	21	Potential
	Transmembrane	147 – 167	21	Potential
	Transmembrane	180 – 200	21	Potential
	Transmembrane	218 – 238	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8PVW4-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: A328ADE9E99406F9	FASTA	259	28,989
	10 20 30 40 50 60 MAYFSGLSDA LRLTFVQIMI LSAIAVVIFL YGMIGNFQKW GAGVTGYALE PPTGKKGSAI 70 80 90 100 110 120 RFLKTWWAQV RAESHHHGKP ILEVLILDIF FQRRILKRSP IRWFMHFTIF AGWMSLFALS 130 140 150 160 170 180 GLMFAVEMTE KIGIELPFTP AEFREMLSLP NYIFGYILLI GVMIAVVRRL FVSEVREASI 190 200 210 220 230 240 MYDWVLLGGV FIVTISGFIA DGIRTGIIWG FGLDPVTAPP AALFHSVISL LFCIAYIPYS 250 KYIHVIATPL AILANKGGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.
[2]	"The F420H2:heterodisulfide oxidoreductase system from Methanosarcina species. 2-Hydroxyphenazine mediates electron transfer from F420H2 dehydrogenase to heterodisulfide reductase." Baeumer S., Murakami E., Brodersen J., Gottschalk G., Ragsdale S.W., Deppenmeier U. FEBS Lett. 428:295-298(1998) [PubMed: 9654152] [Abstract] Cited for: FUNCTION, SOURCE OF ELECTRONS. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008384 Genomic DNA. Translation: AAM31539.1.
RefSeq	NP_633867.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1480185.
GenomeReviews	Gene locus MM_1843 in contig AE008384_GR.
KEGG	mma:MM_1843.
NMPDR	fig|192952.1.peg.1843.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8PVW4.
OMA	Q8PVW4. REASIMY.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM1843-MON.
BRENDA	1.8.98.1. 261165.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

HDRE_METMA

Accession

Primary (citable) accession number: Q8PVW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)