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Protein

2-phospho-L-lactate transferase

Gene

cofD

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the 2-phospholactate moiety from lactyl (2) diphospho-(5')guanosine (LPPG) to 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) with the formation of the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) and GMP.UniRule annotation

Catalytic activityi

(2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin = guanosine 5'-phosphate + coenzyme F420-0.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: coenzyme F420 biosynthesis

This protein is involved in the pathway coenzyme F420 biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway coenzyme F420 biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BRENDAi2.7.8.28. 3270.
UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
2-phospho-L-lactate transferaseUniRule annotation (EC:2.7.8.28UniRule annotation)
Alternative name(s):
LPPG:FO 2-phospho-L-lactate transferaseUniRule annotation
Gene namesi
Name:cofDUniRule annotation
Ordered Locus Names:MM_1874
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001457741 – 3032-phospho-L-lactate transferaseAdd BLAST303

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi192952.MM_1874.

Structurei

Secondary structure

1303
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi9 – 17Combined sources9
Turni18 – 20Combined sources3
Helixi23 – 25Combined sources3
Beta strandi27 – 30Combined sources4
Beta strandi36 – 38Combined sources3
Beta strandi41 – 43Combined sources3
Helixi45 – 53Combined sources9
Turni54 – 56Combined sources3
Turni60 – 63Combined sources4
Beta strandi64 – 66Combined sources3
Helixi72 – 79Combined sources8
Helixi90 – 104Combined sources15
Helixi109 – 120Combined sources12
Beta strandi124 – 129Combined sources6
Beta strandi135 – 142Combined sources8
Beta strandi144 – 146Combined sources3
Helixi147 – 151Combined sources5
Turni152 – 156Combined sources5
Beta strandi160 – 166Combined sources7
Turni167 – 170Combined sources4
Helixi175 – 183Combined sources9
Beta strandi186 – 189Combined sources4
Turni194 – 197Combined sources4
Helixi199 – 203Combined sources5
Helixi207 – 212Combined sources6
Beta strandi214 – 219Combined sources6
Beta strandi226 – 230Combined sources5
Helixi232 – 235Combined sources4
Helixi237 – 239Combined sources3
Helixi245 – 252Combined sources8
Turni253 – 255Combined sources3
Beta strandi258 – 262Combined sources5
Helixi263 – 265Combined sources3
Helixi269 – 275Combined sources7
Beta strandi278 – 282Combined sources5
Helixi289 – 303Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C3DX-ray2.50A/B/C/D1-303[»]
3C3EX-ray3.00A/B/C/D1-303[»]
3CGWX-ray3.10A1-303[»]
ProteinModelPortaliQ8PVT6.
SMRiQ8PVT6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8PVT6.

Family & Domainsi

Sequence similaritiesi

Belongs to the CofD family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04395. Archaea.
COG0391. LUCA.
HOGENOMiHOG000230060.
KOiK11212.
OMAiHFQEYWV.

Family and domain databases

CDDicd07186. CofD_like. 1 hit.
HAMAPiMF_01257. CofD. 1 hit.
InterProiIPR002882. CofD/UPF0052.
IPR010115. P-lactate_Trfase.
[Graphical view]
PfamiPF01933. UPF0052. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01819. F420_cofD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8PVT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIFSGGTGT PKLLDGLKEI LPEEELTVVV NTAEDLWVSG NLISPDLDTV
60 70 80 90 100
LYLFSDQIDR KRWWGIENDT FGTYERMKEL GIEEGLKLGD RDRATHIIRS
110 120 130 140 150
NIIRDGASLT DSTVKLSSLF GIKANILPMS DDPVSTYIET AEGIMHFQDF
160 170 180 190 200
WIGKRGEPDV RGVDIRGVSE ASISPKVLEA FEKEENILIG PSNPITSIGP
210 220 230 240 250
IISLPGMREL LKKKKVVAVS PIIGNAPVSG PAGKLMPACG IEVSSMGVAE
260 270 280 290 300
YYQDFLDVFV FDERDRADEF AFERLGCHAS RADTLMTSTE KSKELAEIVV

QAF
Length:303
Mass (Da):33,357
Last modified:October 1, 2002 - v1
Checksum:iDBBBF4BAB5659C63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM31570.1.
RefSeqiWP_011033809.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM31570; AAM31570; MM_1874.
GeneIDi1480216.
KEGGimma:MM_1874.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM31570.1.
RefSeqiWP_011033809.1. NC_003901.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C3DX-ray2.50A/B/C/D1-303[»]
3C3EX-ray3.00A/B/C/D1-303[»]
3CGWX-ray3.10A1-303[»]
ProteinModelPortaliQ8PVT6.
SMRiQ8PVT6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_1874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM31570; AAM31570; MM_1874.
GeneIDi1480216.
KEGGimma:MM_1874.

Phylogenomic databases

eggNOGiarCOG04395. Archaea.
COG0391. LUCA.
HOGENOMiHOG000230060.
KOiK11212.
OMAiHFQEYWV.

Enzyme and pathway databases

UniPathwayiUPA00071.
BRENDAi2.7.8.28. 3270.

Miscellaneous databases

EvolutionaryTraceiQ8PVT6.

Family and domain databases

CDDicd07186. CofD_like. 1 hit.
HAMAPiMF_01257. CofD. 1 hit.
InterProiIPR002882. CofD/UPF0052.
IPR010115. P-lactate_Trfase.
[Graphical view]
PfamiPF01933. UPF0052. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01819. F420_cofD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOFD_METMA
AccessioniPrimary (citable) accession number: Q8PVT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.