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Protein

DNA ligase 1

Gene

lig1

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei244ATPUniRule annotation1
Active sitei246N6-AMP-lysine intermediateUniRule annotation1
Binding sitei251ATPUniRule annotation1
Binding sitei266ATPUniRule annotation1
Binding sitei296ATPUniRule annotation1
Binding sitei342ATPUniRule annotation1
Binding sitei419ATPUniRule annotation1
Binding sitei425ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 1UniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] 1UniRule annotation
Gene namesi
Name:lig1UniRule annotation
Ordered Locus Names:MM_1895
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003652591 – 579DNA ligase 1Add BLAST579

Interactioni

Protein-protein interaction databases

STRINGi192952.MM_1895.

Structurei

3D structure databases

ProteinModelPortaliQ8PVR7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01347. Archaea.
COG1793. LUCA.
HOGENOMiHOG000036008.
KOiK10747.
OMAiPQVVIEV.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
HAMAPiMF_00407. DNA_ligase. 1 hit.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8PVR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFKELAELF EELEKTTSHR EIVRKISEFF KNLRGDEVKD SAYLFLGSTG
60 70 80 90 100
PAFENTTLGI KDMLAIRAIA GAYGVTREDV RKRYARTGDL GDVAFELSKK
110 120 130 140 150
RESSLTIEDV FQRLLQIRET SGKGSQEEKT ALFSDILQKA TPEEGKYIVR
160 170 180 190 200
LVLGRLRLGF GDQFLLEAFA IAFTGDKKHA AKIKESYSVC TDIGELAKIL
210 220 230 240 250
AENGARATGF ISIKPGRPVK SMLSQRVESF EELEKRVKGK KAAEEKYDGE
260 270 280 290 300
RVQVHKTGEG IKAFSRRLED ITSQYPEIIE DVRKTVPANE IVLDGEIVAY
310 320 330 340 350
AELERNGNRI EEFYPFQNLM QRRRKYEIEN YRKKCPVAVF FFDILYLNGE
360 370 380 390 400
PLLKRPYPER RALLEMNVVE SGIIRLSKRI VTESVEEIED FFNETIEKGL
410 420 430 440 450
EGIVVKSMSS NSYYEAGKRS WFWFKWKQEY SEGMRETFDL VVVGSYYGRG
460 470 480 490 500
RRKGSFGALL CAVLNKEGQR FETLTKVGTG FTEADAEEIN RLLSDHIVSE
510 520 530 540 550
IPKGVSIKKG MLPDIFIEPA VVIEVLGSEI TNSPGHTAGE GEEETGLALR
560 570
FPRFLRIRHD KTPYDAMTVK EVRDLKDGT
Length:579
Mass (Da):65,867
Last modified:October 1, 2002 - v1
Checksum:i9E11E66075B8DEEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM31591.1.
RefSeqiWP_011033828.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM31591; AAM31591; MM_1895.
GeneIDi24878633.
KEGGimma:MM_1895.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM31591.1.
RefSeqiWP_011033828.1. NC_003901.1.

3D structure databases

ProteinModelPortaliQ8PVR7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_1895.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM31591; AAM31591; MM_1895.
GeneIDi24878633.
KEGGimma:MM_1895.

Phylogenomic databases

eggNOGiarCOG01347. Archaea.
COG1793. LUCA.
HOGENOMiHOG000036008.
KOiK10747.
OMAiPQVVIEV.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
HAMAPiMF_00407. DNA_ligase. 1 hit.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNLI1_METMA
AccessioniPrimary (citable) accession number: Q8PVR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.