Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8PVR7 (DNLI1_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase 1

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] 1
Gene names
Name:lig1
Ordered Locus Names:MM_1895
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length579 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 579579DNA ligase 1 HAMAP-Rule MF_00407
PRO_0000365259

Sites

Active site2461N6-AMP-lysine intermediate By similarity
Binding site2441ATP By similarity
Binding site2511ATP By similarity
Binding site2661ATP By similarity
Binding site2961ATP By similarity
Binding site3421ATP By similarity
Binding site4191ATP By similarity
Binding site4251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8PVR7 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 9E11E66075B8DEEC

FASTA57965,867
        10         20         30         40         50         60 
MRFKELAELF EELEKTTSHR EIVRKISEFF KNLRGDEVKD SAYLFLGSTG PAFENTTLGI 

        70         80         90        100        110        120 
KDMLAIRAIA GAYGVTREDV RKRYARTGDL GDVAFELSKK RESSLTIEDV FQRLLQIRET 

       130        140        150        160        170        180 
SGKGSQEEKT ALFSDILQKA TPEEGKYIVR LVLGRLRLGF GDQFLLEAFA IAFTGDKKHA 

       190        200        210        220        230        240 
AKIKESYSVC TDIGELAKIL AENGARATGF ISIKPGRPVK SMLSQRVESF EELEKRVKGK 

       250        260        270        280        290        300 
KAAEEKYDGE RVQVHKTGEG IKAFSRRLED ITSQYPEIIE DVRKTVPANE IVLDGEIVAY 

       310        320        330        340        350        360 
AELERNGNRI EEFYPFQNLM QRRRKYEIEN YRKKCPVAVF FFDILYLNGE PLLKRPYPER 

       370        380        390        400        410        420 
RALLEMNVVE SGIIRLSKRI VTESVEEIED FFNETIEKGL EGIVVKSMSS NSYYEAGKRS 

       430        440        450        460        470        480 
WFWFKWKQEY SEGMRETFDL VVVGSYYGRG RRKGSFGALL CAVLNKEGQR FETLTKVGTG 

       490        500        510        520        530        540 
FTEADAEEIN RLLSDHIVSE IPKGVSIKKG MLPDIFIEPA VVIEVLGSEI TNSPGHTAGE 

       550        560        570 
GEEETGLALR FPRFLRIRHD KTPYDAMTVK EVRDLKDGT 

« Hide

References

[1]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008384 Genomic DNA. Translation: AAM31591.1.
RefSeqNP_633919.1. NC_003901.1.

3D structure databases

ProteinModelPortalQ8PVR7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING192952.MM_1895.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM31591; AAM31591; MM_1895.
GeneID1480237.
KEGGmma:MM_1895.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMACTDIGEL.

Enzyme and pathway databases

BioCycMMAZ192952:GCK2-1944-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI1_METMA
AccessionPrimary (citable) accession number: Q8PVR7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: October 1, 2002
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families