Reviewed, UniProtKB/Swiss-Prot Q8PVP6 (SYK2_METMA)

Names and origin

Protein names

Recommended name:
    Lysyl-tRNA synthetase 2
    EC=6.1.1.6
Alternative name(s):
    Lysine--tRNA ligase 2
      Short name=LysRS 2

Gene names

Name:	lysS2
Ordered Locus Names:	MM_1916

Organism

Methanosarcina mazei (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

2209 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity. HAMAP MF_00252
Subunit structure	Homodimer By similarity. HAMAP MF_00252
Subcellular location	Cytoplasm HAMAP MF_00252.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	Lysyl-tRNA synthetase 2 HAMAP MF_00252		PRO_0000152712
Sites
	Metal binding	421	1	Magnesium 1 By similarity
	Metal binding	428	1	Magnesium 1 By similarity
	Metal binding	428	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PVP6-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6DF668BA4E971849	FASTA	511	58,795
	10 20 30 40 50 60 MTMEINNTDP FEKMPLPDDS GLSGSGAFDD SKLAKLNGVI SQGLNPYPYK FEKDEDICEI 70 80 90 100 110 120 LEKFEDFEKN EGLTVRTAGR LYNIRKHGKM IFADLGDQAG RVQVLVRKGN LPDEEFEIFK 130 140 150 160 170 180 NLVDSGDIIG VQGDLFRTKR GENSISVSEF SLLSKSLCAL PEKFHGLKDV ETRYRKRYLD 190 200 210 220 230 240 LIVNAEKREI FVMRSKLISE IRRFLTDREF LEFETPILQT VYGGANARPF TTFHNCLGQN 250 260 270 280 290 300 LFLRIAPELY LKRLVVGGYE KVFEICKNFR NEDIDTTHNP EFTMIEVYEA YRDYNDMMDL 310 320 330 340 350 360 TESLVSELVF KLTGSYEVQM GEKTINLRSP WKRISMEDAL KEYAGLDIFA HSIEDLKKIA 370 380 390 400 410 420 IENRIEDYEK AKSHGEFLAL LFEGLVEDKL IDPTFIYDFP VENSPLAKNH REKAGFVERF 430 440 450 460 470 480 ELFLNGWELA NGYSELNDPL EQEKRFEEQD KKRKLGDLEA QTVDYDFINA LGYGLPPTGG 490 500 510 MGLGIDRLTM ILSGLESIKE VILFPQMKRE D

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM31612.1.
RefSeq	NP_633940.1.
3D structure databases
SMR	Q8PVP6. Positions 31-510.
ModBase	Search...
Genome annotation databases
GeneID	1480258.
GenomeReviews	Gene locus MM_1916 in contig AE008384_GR.
KEGG	mma:MM_1916.
NMPDR	fig|192952.1.peg.1916.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG631383.
OMA	ANEESRH.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM1916-MONOMER.
BRENDA	6.1.1.6. 261165.
Family and domain databases
HAMAP	MF_00252. Lys_tRNA_synth_class2. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR002313. Lys-tRNA-synth_II. IPR018149. Lys-tRNA-synth_II_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF4. tRNA-synt_lys_2. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
TIGRFAMs	TIGR00499. lysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK2_METMA

Accession

Primary (citable) accession number: Q8PVP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)