Q8PVJ7 (MDH_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	MM_1966

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina mazei

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_0000113487
Regions
	Nucleotide binding	8 – 13	6	NAD By similarity
	Nucleotide binding	117 – 119	3	NAD By similarity
Sites
	Active site	174	1	Proton acceptor By similarity
	Binding site	32	1	NAD By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	94	1	NAD By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	150	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PVJ7 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 93C68177714AF6D3	FASTA	307	32,786
	10 20 30 40 50 60 MVKISVIGAG NVGSTTVQRL AELEPGEIVM TDIVEGMPQG KALDLMQAGA INGYDTRITG 70 80 90 100 110 120 TNDYADIANS DLVIITAGIA RKPGMSREDL IKTNSKIIGD VAGNIAKYAP NSIVINVTNP 130 140 150 160 170 180 LDIITYVAMK ATGFDPEKVF GMSGVLDAGR FASFIAEELK CSKRDVEAMV IGGHGDLMVP 190 200 210 220 230 240 LPQYTTVSGI PLPELLPEKT IDRLVERTVN GGAEIVELLK QGSAFYAPSA AIVRMAEAVI 250 260 270 280 290 300 KDSRRVLPAS AYLEGQYGQK GIYFGVPVKL GANGIEEILE LKLEDSQCEI LKKSSETIRK GISKLEI

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM31662.1.
RefSeq	NP_633990.1. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PVJ7.
SMR	Q8PVJ7. Positions 2-305.
ModBase	Search...
Protein-protein interaction databases
STRING	192952.MM_1966.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM31662; AAM31662; MM_1966.
GeneID	1480308.
KEGG	mma:MM_1966.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213794.
KO	K00024.
OMA	DAMTYVM.
ProtClustDB	PRK06223.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_METMA

Accession

Primary (citable) accession number: Q8PVJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program