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Protein

Quinolinate synthase A

Gene

nadA

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.UniRule annotation

Catalytic activityi

Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from iminoaspartate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Quinolinate synthase A (nadA)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from iminoaspartate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei24 – 241IminoaspartateUniRule annotation
Binding sitei41 – 411Iminoaspartate; via amide nitrogenUniRule annotation
Binding sitei112 – 1121IminoaspartateUniRule annotation
Binding sitei129 – 1291IminoaspartateUniRule annotation
Binding sitei197 – 1971IminoaspartateUniRule annotation
Binding sitei214 – 2141IminoaspartateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-2127-MONOMER.
UniPathwayiUPA00253; UER00327.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinolinate synthase AUniRule annotation (EC:2.5.1.72UniRule annotation)
Gene namesi
Name:nadAUniRule annotation
Ordered Locus Names:MM_2073
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Quinolinate synthase APRO_0000155805Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi192952.MM_2073.

Structurei

3D structure databases

ProteinModelPortaliQ8PV98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the quinolinate synthase A family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04459. Archaea.
COG0379. LUCA.
HOGENOMiHOG000222770.
KOiK03517.
OMAiIAHPECE.

Family and domain databases

HAMAPiMF_00568. NadA_type2. 1 hit.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8PV98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQADLTKRI NELKLKRNAV ILSHYYSRPE VQDIADFVGD SLALSQEAVR
60 70 80 90 100
QDADVIVFCG VHFMGESAAI LSPNKTVLLP EIDATCPMAD MVNVEGLKIL
110 120 130 140 150
KEKHPEAPVV SYVNSSAAIK AESYICCTSA NAVEVVNSLD ADEVIFVPDK
160 170 180 190 200
NLAAYVASRT DKKIIPWEGH CPTHHQILRE DVLKMKEKHP RAEFIAHPEC
210 220 230 240 250
RPDVLELADH VASTRGMIVH AERSPAKEFI IGTECGLIHG LLKAVPEKTY
260 270 280 290 300
YCVSEFACCP SMKMVNLEKV LTSLEKMQQR VIVPEDVRAR AKEALDRMLA

VKIN
Length:304
Mass (Da):33,800
Last modified:October 25, 2002 - v1
Checksum:i9493E1AD2B0966D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM31769.1.
RefSeqiWP_011034005.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM31769; AAM31769; MM_2073.
GeneIDi24881759.
KEGGimma:MM_2073.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM31769.1.
RefSeqiWP_011034005.1. NC_003901.1.

3D structure databases

ProteinModelPortaliQ8PV98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_2073.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM31769; AAM31769; MM_2073.
GeneIDi24881759.
KEGGimma:MM_2073.

Phylogenomic databases

eggNOGiarCOG04459. Archaea.
COG0379. LUCA.
HOGENOMiHOG000222770.
KOiK03517.
OMAiIAHPECE.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.
BioCyciMMAZ192952:GCK2-2127-MONOMER.

Family and domain databases

HAMAPiMF_00568. NadA_type2. 1 hit.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADA_METMA
AccessioniPrimary (citable) accession number: Q8PV98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: October 25, 2002
Last modified: November 11, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.