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Protein

Probable tRNA pseudouridine synthase B

Gene

truB

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)
Alternative name(s):
tRNA pseudouridine(55) synthaseUniRule annotation
Short name:
Psi55 synthaseUniRule annotation
tRNA pseudouridylate synthaseUniRule annotation
tRNA-uridine isomeraseUniRule annotation
Gene namesi
Name:truBUniRule annotation
Ordered Locus Names:MM_2154
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001219611 – 338Probable tRNA pseudouridine synthase BAdd BLAST338

Interactioni

Protein-protein interaction databases

STRINGi192952.MM_2154.

Structurei

3D structure databases

ProteinModelPortaliQ8PV20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini245 – 320PUAUniRule annotationAdd BLAST76

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00987. Archaea.
COG0130. LUCA.
HOGENOMiHOG000231224.
KOiK11131.
OMAiLFRIGCE.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01081. TruB_arch. 1 hit.
InterProiIPR012960. Dyskerin-like.
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR026326. TruB_arch.
IPR032819. TruB_C.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTiSM01136. DKCLD. 1 hit.
SM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8PV20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSAGKLPSE VERTLVRKSG AWTNPAYGCP PEKRPIHEYI EKGVVNIDKP
60 70 80 90 100
RGPTSHEVAA WVKAILGVHT AGHAGSLDPK VTGLLPTLLG KATLAVPALR
110 120 130 140 150
LSGKEYICHL KLHRAMPQKL VRQVCEEFTG PIYQMPPIKS AVKRVIRVRT
160 170 180 190 200
IYHLEVLEIE GTSVLFRVGC EAGTYIRKLC HDIGLALGCG GHMQGLRRTK
210 220 230 240 250
AGPFTEKTLI TLHELKDAYV FWKEDGDESE LRRVIRPMES AVSHLPKIIL
260 270 280 290 300
RDSAVDAICS GASLAVPGIT GLDSNLAEGE LTGLFTLKGE LVALAKAKMT
310 320 330
TEEILKASAG IAASPIRVLM EAGTYPRGWT KKEEKVQL
Length:338
Mass (Da):36,775
Last modified:October 1, 2002 - v1
Checksum:iE408052A18D92066
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM31850.1.
RefSeqiWP_011034085.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM31850; AAM31850; MM_2154.
GeneIDi1480496.
KEGGimma:MM_2154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM31850.1.
RefSeqiWP_011034085.1. NC_003901.1.

3D structure databases

ProteinModelPortaliQ8PV20.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_2154.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM31850; AAM31850; MM_2154.
GeneIDi1480496.
KEGGimma:MM_2154.

Phylogenomic databases

eggNOGiarCOG00987. Archaea.
COG0130. LUCA.
HOGENOMiHOG000231224.
KOiK11131.
OMAiLFRIGCE.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01081. TruB_arch. 1 hit.
InterProiIPR012960. Dyskerin-like.
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR026326. TruB_arch.
IPR032819. TruB_C.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTiSM01136. DKCLD. 1 hit.
SM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRUB_METMA
AccessioniPrimary (citable) accession number: Q8PV20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.