Carbon monoxide dehydrogenase 2 - Methanosarcina mazei

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Reviewed, UniProtKB/Swiss-Prot Q8PUN1 (COOS2_METMA)

Last modified June 16, 2009. Version 48. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Carbon monoxide dehydrogenase 2
      Short name=CODH 2
    EC=1.2.99.2

Gene names

Name:	cooS2
Ordered Locus Names:	MM_2301

Organism

Methanosarcina mazei (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	634 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	CO + H₂O + A = CO₂ + AH₂.
Cofactor	Binds 3 4Fe-4S clusters per homodimer By similarity. Binds 2 nickel-iron-sulfur clusters per homodimer By similarity.
Subunit structure	Homodimer By similarity.
Domain	Cluster B is an all-cysteinyl-liganded 4Fe4S cluster; cluster C is a mixed Ni-Fe-S cluster which appears to be the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe4S cluster that bridges the two subunits of the CODH dimer By similarity.
Sequence similarities	Belongs to the Ni-containing carbon monoxide dehydrogenase family.
Caution	This protein lacks the conserved Cys in positions 52 and 300; they are replaced by an Arg and a Glu, respectively. It is therefore possible that the C- and D-clusters are either altered or missing in this protein.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	generation of precursor metabolites and energy Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-monoxide dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

634

Carbon monoxide dehydrogenase 2

PRO_0000157146

Sites

Metal binding

1

Iron-sulfur 1 (4Fe-4S); shared with dimeric partner By similarity

Metal binding

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Metal binding

1

Nickel-iron-sulfur By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8PUN1-1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 4C2B004AAB767190
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634

69,220

        10         20         30         40         50         60 
MDKERISYHE SVQKMYERIK ADNMTNVWDR YEAQGIGGVP DRRCTFCMAG ARCDLCSNGP 

        70         80         90        100        110        120 
CRSDASKDKR GVCGITADGM AMRMMLLRNV MGASTYHYHT DQTIRTLRET ARNKTPYSIR 

       130        140        150        160        170        180 
EPEKLKTFAN RLGIDISGSD AEIALNLCEF VEKDFNRPAY EPSRIVEILA PPERKKRWEE 

       190        200        210        220        230        240 
LGIFPGGIYG EMMLSTSSCL TNVDGYYVSL ALKAMRLGIA MAYQSQIVNE YCQDVLFGIP 

       250        260        270        280        290        300 
RPHTMRVDLG VLDPEYVNVL PNGHEPFLGF AMVQLARKPE WQEKAKAAGA KGLRVIASIE 

       310        320        330        340        350        360 
TGQEMIQRWE EDDVFYGFTG NWISQEAVLA SRCVDLFAAD MNCSLPVAPL YAEKYNFKLM 

       370        380        390        400        410        420 
PVSDLVAFEG IEERLNYDPV EAEEQAAKLL DMAVENFKNR NSSGEAALNF PAGEAVVGFS 

       430        440        450        460        470        480 
TESILDALGG TLDPLLDAIK SGAIKGVVGM VSCTTLRDYG QDVHSVAVVK ELIKRNILVL 

       490        500        510        520        530        540 
SMGCGNAAMQ VAGLCSPEAR EYAGDSLKAV CEALGVPPVL SYGTCTDTGR LADLLGAISG 

       550        560        570        580        590        600 
ALGGVPVPDL PVAAAAPEYM EQKATIDAIF ALALGLYTYV NPVPTVTGAP NLVKLLTEDC 

       610        620        630 
REVTGGLLNV ETDAVKAVDG IEQHIMEKRK KLGI

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R.

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Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.

Cross-references

Sequence databases
	AE008384 Genomic DNA. Translation: AAM31997.1.
RefSeq	NP_634325.1.
3D structure databases
HSSP	HSSP built from PDB template 1JJY based on UniProtKB Q9F8A8.
ModBase	Search...
Genome annotation databases
GeneID	1480643.
GenomeReviews	Gene locus MM_2301 in contig AE008384_GR.
KEGG	mma:MM_2301.
NMPDR	fig\|192952.1.peg.2301.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8PUN1.
OMA	Q8PUN1. YMEQKAT.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM2301-MON.
BRENDA	1.2.99.2. 261165.
Family and domain databases
InterPro	IPR016101. CO_DH_a-bundle. IPR010047. CO_DH_cat. IPR004137. Prismane. IPR016099. Prismane-like_a/b-sand. [Graphical view]
Gene3D	G3DSA:1.20.1270.30. CO_DH_a-bundle. 1 hit. G3DSA:3.40.50.2030. Prismane-like_a/b-sand. 2 hits.
Pfam	PF03063. Prismane. 1 hit. [Graphical view]
PIRSF	PIRSF005023. CODH. 1 hit.
TIGRFAMs	TIGR01702. CO_DH_cata. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COOS2_METMA

Accession

Primary (citable) accession number: Q8PUN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents