Q8PUM7 (GATD_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit D
Short name=Glu-ADT subunit D
EC=6.3.5.-

Gene names

Name:	gatD
Ordered Locus Names:	MM_2306

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina mazei

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate By similarity. HAMAP-Rule MF_00586
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00586
Subunit structure	Heterodimer of GatD and GatE By similarity.
Sequence similarities	Belongs to the asparaginase 1 family. GatD subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro regulation of translational fidelity Inferred from electronic annotation. Source: InterPro translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparaginase activity Inferred from electronic annotation. Source: InterPro glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Glutamyl-tRNA(Gln) amidotransferase subunit D HAMAP-Rule MF_00586		PRO_0000140054
Sites
	Active site	95	1	By similarity
	Active site	171	1	By similarity
	Active site	172	1	By similarity
	Active site	248	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PUM7 [UniParc]. Last modified October 25, 2002. Version 1. Checksum: C96DEF778096625D	FASTA	425	45,845
	10 20 30 40 50 60 MEFKQGDRVR IEKNGTVYEG KVMPSMEGYI TIKMKSGYNA GFSIDKVSIT PLENNGEAAN 70 80 90 100 110 120 GGNGGKNGQK EPEPAKEKVS KPGLPKVSIL STGGTIASKI DYRTGAVTSQ FTADDILAAI 130 140 150 160 170 180 PELKEIADFK GRAISSILSE NMDPDSWQNL ARAVVEEIEA GADGIIVTHG TDTMMYSAAA 190 200 210 220 230 240 LSFMIETPVP IVFVGSQRSA DRPSSDNAMN AICAARVAIS DIAEVVVVMH GTSSDDYCEI 250 260 270 280 290 300 HRGTKVRKMH TSRRDAFKSV NSLPIGTVDY DTGEIKTFIE YTGRGEKSLK FKPGMEPKCA 310 320 330 340 350 360 IVKFTPGADP SVLDCYVDGG YKGLVLEGTG LGHVSTKWIP FIRRAVDAKM PVIVTSQCLN 370 380 390 400 410 420 GRVCDRVYDT GRDMLKAGAI EGEDTLPETA LVKLMWVLGQ TDEFEKAVSM LGENLSGEIN ECTLR

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM32002.1.
RefSeq	NP_634330.1. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PUM7.
ModBase	Search...
Protein-protein interaction databases
STRING	192952.MM_2306.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM32002; AAM32002; MM_2306.
GeneID	1480648.
KEGG	mma:MM_2306.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0252.
HOGENOM	HOG000227975.
KO	K09482.
OMA	KKEHASN.
ProtClustDB	PRK04183.
Family and domain databases
HAMAP	MF_00586. GatD.
InterPro	IPR006033. AsnASEI. IPR006034. Asparaginase/glutaminase. IPR020827. Asparaginase/glutaminase_CS. IPR011878. GatD. [Graphical view]
Pfam	PF00710. Asparaginase. 1 hit. [Graphical view]
PIRSF	PIRSF001220. L-ASNase_gatD. 1 hit.
PRINTS	PR00139. ASNGLNASE.
SMART	SM00870. Asparaginase. 1 hit. [Graphical view]
SUPFAM	SSF53774. Asp/Glutamnse. 1 hit.
TIGRFAMs	TIGR00519. asnASE_I. 1 hit. TIGR02153. gatD_arch. 1 hit.
PROSITE	PS00144. ASN_GLN_ASE_1. 1 hit. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATD_METMA

Accession

Primary (citable) accession number: Q8PUM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 25, 2002
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program