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Protein

Type 2 DNA topoisomerase 6 subunit B

Gene

top6B

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Relaxes both positive and negative superturns and exhibits a strong decatenase activity.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei48ATPUniRule annotation1
Binding sitei80ATPUniRule annotation1
Binding sitei435ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi101 – 102ATPUniRule annotation2
Nucleotide bindingi111 – 118ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi5.99.1.3 3270

Names & Taxonomyi

Protein namesi
Recommended name:
Type 2 DNA topoisomerase 6 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Type II DNA topoisomerase VI subunit BUniRule annotation
Short name:
TopoVI-BUniRule annotation
Gene namesi
Name:top6BUniRule annotation
Ordered Locus Names:MM_2417
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001454631 – 621Type 2 DNA topoisomerase 6 subunit BAdd BLAST621

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two Top6A and two Top6B chains.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
top6AQ8PUB72EBI-9026766,EBI-9026768

Protein-protein interaction databases

DIPiDIP-29415N
IntActiQ8PUB8, 1 interactor
STRINGi192952.MM_2417

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Q2EX-ray4.00B1-621[»]
ProteinModelPortaliQ8PUB8
SMRiQ8PUB8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8PUB8

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6B family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01165 Archaea
COG1389 LUCA
HOGENOMiHOG000225919
KOiK03167
OMAiIQSRGQQ
OrthoDBiPOG093Z04O3

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
cd00823 TopoIIB_Trans, 1 hit
Gene3Di3.30.230.10, 1 hit
3.30.565.10, 1 hit
HAMAPiMF_00322 Top6B, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR010979 Ribosomal_S13-like_H2TH
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR005734 TopoVI_B
IPR015320 TopoVI_B_transducer
PANTHERiPTHR10871:SF4 PTHR10871:SF4, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF09239 Topo-VIb_trans, 1 hit
PIRSFiPIRSF006553 TopoVI_B, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD013790 TopoVI_B_transducer, 1 hit
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF46946 SSF46946, 1 hit
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
TIGRFAMsiTIGR01052 top6b, 1 hit

Sequencei

Sequence statusi: Complete.

Q8PUB8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METPIAEELA KKQKSISVAE FFEKNRQILG FDSAPRSLIT TVKEAVDNAL
60 70 80 90 100
DACEEAGILP DILVQVERTG PDYVTVIIED NGPGIVREQI PKVFAKLLYG
110 120 130 140 150
SRFHALKQSR GQQGIGISAA VLYAQMTAGR HTKILSKTSP TAPAHYYELM
160 170 180 190 200
INTSTNEPDI LVDEVRDWFR PHGTQIELEM RAAYVKGRRQ SIYEYLKATA
210 220 230 240 250
IVNPHARITL IDPDGNEEVF ERATDKMPEP AEEILPHPEG IELGTLMKML
260 270 280 290 300
HYTERQKLAP FLRYSFCKIG LLTAEEICKA AGLDPEIDPH ALGRHEARKL
310 320 330 340 350
IEAFEKVKIM APPTDCLSPI GEDLIYRGLE KETTVDFIAT STRKPAVYSG
360 370 380 390 400
NPFVVEVGMA YGGNLPKEEK ISIMRFANRV PLLYQQGGCV TTHAVEDIKW
410 420 430 440 450
KQYGLNQPGG GIPVGPVILL IHVASINVPF TSESKDAIAD IPVIKEEIDL
460 470 480 490 500
AIKEVARKLK HYLSKQSNLK KRREKEIIIT KVLPKLAAKV AHVLEKDVPD
510 520 530 540 550
INPVVAKIMG NLLVHRVIKN NGDGTVDVAI KVKNFGTSAY SFRVHEMLPC
560 570 580 590 600
KVSGAKPEPK VVTMGNDYDY VWDISASAGS SKVLSYKIES ASEEELQKLP
610 620
QLIVEGIEEE LVTGAKAFKG V
Length:621
Mass (Da):68,760
Last modified:October 10, 2002 - v1
Checksum:i7D2FEC4F2FA68AD6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA Translation: AAM32113.1
RefSeqiWP_011034341.1, NC_003901.1

Genome annotation databases

EnsemblBacteriaiAAM32113; AAM32113; MM_2417
GeneIDi24865418
KEGGimma:MM_2417
PATRICifig|192952.21.peg.2765

Similar proteinsi

Entry informationi

Entry nameiTOP6B_METMA
AccessioniPrimary (citable) accession number: Q8PUB8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: October 10, 2002
Last modified: May 23, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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