Q8PUB5 (Q8PUB5_METMA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A HAMAP MF_01897
EC=5.99.1.3 HAMAP MF_01897

Gene names

Name:	gyrA HAMAP MF_01897
Ordered Locus Names:	MM_2420

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP] EMBL AAM32116.1

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	900 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity. HAMAP MF_01897
Subcellular location	Cytoplasm By similarity HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP MF_01897

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_01897
Ligand	ATP-binding HAMAP MF_01897 DNA-binding HAMAP MF_01897 Nucleotide-binding
Molecular function	Isomerase Topoisomerase HAMAP MF_01897
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8PUB5 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: A471287E39124225	FASTA	900	101,311
	10 20 30 40 50 60 MDTGPEEPED EEPVRAGVTP ILIDDEMKRS YINYAMSVIV GRALPDARDG LKPVHRRILF 70 80 90 100 110 120 SMNESGITHD KPYKKSARIV GDVLGKYHPH GDSAVYDTIV RMVQDFSLRY PLIDGQGNFG 130 140 150 160 170 180 SIDGDSAAAM RYTEVRMDRI AEEMLVDIEK ETVPFIPNYD GSLKEPEVLP ARLPNLLING 190 200 210 220 230 240 STGIAVGMAT NMPPHNISEV IDGILMLIEN PETQISELMT VIKGPDFPTG AHILGTAGIR 250 260 270 280 290 300 SAYMTGRGPI RMRAVAEIQE LKKDRQQIIV TELPYQVNKA KLIENIAQLV REKVINGISD 310 320 330 340 350 360 LRDESDREGI RVVVELSRGV NPNVVLNQLY KHTQMATTFG VINLALVDGK PKELNLKQLL 370 380 390 400 410 420 EIYLEYRMEI IQKRTLYDLR KSEERAHILE GLRIALDNID AVVALIKGSA NADQAKQGLI 430 440 450 460 470 480 DNFALDEVQA KAILEMRLQR LTGLETRKIL DELEALVKQI EELRKILASD ELKYEIIRTE 490 500 510 520 530 540 LSELKDKYGD IRRTRIVHNT ENVTDEDLIP EEDVVVTITN SGYIKRIPLD TYTMQRRGGR 550 560 570 580 590 600 GIIGMEMKEE DFVENLFISS THNYILFFTN RGRLYWQKVY EIPEGSRQSR GKAIVNLLEL 610 620 630 640 650 660 QEGEMVNAMI SVEEFAEDKY LFMATRAGTV KKTPLSEFRN PRRAGIIAVS LDEGDELVKV 670 680 690 700 710 720 LLTDGKQEIM MVSKKGKAIR FSEEDVRPMG RTARGVRGMT LEGPDDEVVS LDLVDETTTL 730 740 750 760 770 780 LTVTDNGFGK RTEYSQYPVH RRGGKGVITI VTNLRNGFVS NVKSVAEDDE LMFTSAEGII 790 800 810 820 830 840 IRIPAKDISI QGRNTQGVRV MNIKSGDKVG GMARIKNGKE DEKLKLTALA KKAAKEKVPG 850 860 870 880 890 900 SESEEDESED LDEFEGSDEF GEEPEKEEFE DSESEDSESE DSEEEEEEEE EEEEEKEEEY

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM32116.1.
RefSeq	NP_634444.1. NC_003901.1.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
ProteinModelPortal	Q8PUB5.
SMR	Q8PUB5. Positions 40-496.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1480762.
GenomeReviews	Gene locus MM_2420 in contig AE008384_GR.
KEGG	mma:MM_2420.
NMPDR	fig|192952.1.peg.2420.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG635183.
OMA	TGRGRIY.
PhylomeDB	Q8PUB5.
ProtClustDB	PRK05560.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q8PUB5_METMA

Accession

Primary (citable) accession number: Q8PUB5

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)