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Q8PU59 (FPOH_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
F(420)H(2) oxidoreductase subunit H
Alternative name(s):
F(420)H(2) dehydrogenase subunit H
FPO subunit H
Gene names
Name:fpoH
Ordered Locus Names:MM_2487
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

FPO shuttles electrons from F(420)H2, via FAD and iron-sulfur (Fe-S) centers, to quinones in the F(420)H2:heterodisulfide oxidoreduction chain. The immediate electron acceptor for the enzyme in this species is believed to be methanophenazine. Couples the redox reaction to proton translocation (for every two electrons transferred, 0.9 hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. Ref.1 Ref.3

Subunit structure

FPO is composed of at least 12 different subunits. Subunits fpoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Potential HAMAP MF_01350.

Sequence similarities

Belongs to the complex I subunit 1 family.

Sequence caution

The sequence AAF65735.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandFAD
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionoxidoreductase activity

Inferred from electronic annotation. Source: UniProtKB-KW

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350F(420)H(2) oxidoreductase subunit H HAMAP MF_01350
PRO_0000240123

Regions

Transmembrane21 – 4121Helical; Potential
Transmembrane94 – 11421Helical; Potential
Transmembrane128 – 14821Helical; Potential
Transmembrane173 – 19321Helical; Potential
Transmembrane200 – 22021Helical; Potential
Transmembrane261 – 28121Helical; Potential
Transmembrane285 – 30521Helical; Potential
Transmembrane330 – 35021Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
Q8PU59 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 21F9E161058612A6

FASTA35038,443
        10         20         30         40         50         60 
MTFMAIEIPE FIVPFVPWIR GTVGLVLVGA IFLGGMAAVW IERKLSADIQ LRYGPSRVGK 

        70         80         90        100        110        120 
FGLLQLVADA IKLFTKEDVR PGNADRFLYD NAPVFMLTSL FLMLVAIPVG AVFIDGNLYP 

       130        140        150        160        170        180 
LAVTEMDISI LFIEAVSAIN IFGIFMAAYG SNNKYSLLGA FRNFARMIGY EVPLGIAIVS 

       190        200        210        220        230        240 
VAVMTGSLNI IDITSAQGSF VWNIFLQPIG FVVFFIALMA DLGRLPFDQN ESEEELVAGW 

       250        260        270        280        290        300 
VTEYTGMRFG LVFFAEYMHM ILGSFLVALL FLGGWNVPAF VANNAVLGLI APTGILLLKT 

       310        320        330        340        350 
VLVLMTIIGM RWAVPRFRID QVVDMSWKKL LPLSLLNLAW AVGLGLYLGA

« Hide

References

« Hide 'large scale' references
[1]"The F(420)H(2) dehydrogenase from Methanosarcina mazei is a redox-driven proton pump closely related to NADH dehydrogenases."
Baeumer S., Ide T., Jacobi C., Johann A., Gottschalk G., Deppenmeier U.
J. Biol. Chem. 275:17968-17973(2000) [PubMed: 10751389] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, FPO COMPLEX.
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.
[2]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.
[3]"Purification and properties of an F(420)H(2) dehydrogenase from Methanosarcina mazei Go1."
Abken H.-J., Deppenmeier U.
FEMS Microbiol. Lett. 154:231-237(1997)
Cited for: FUNCTION, FPO COMPLEX, CATALYTIC ACTIVITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF228525 Genomic DNA. Translation: AAF65735.1. Different initiation.
AE008384 Genomic DNA. Translation: AAM32183.1.
RefSeqNP_634511.1. NC_003901.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

TCDB3.D.9.1.1. H+-translocating F420H2 dehydrogenase (F420H2DH) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1480829.
GenomeReviewsGene locus MM_2487 in contig AE008384_GR.
KEGGmma:MM_2487.
NMPDRfig|192952.1.peg.2487.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG727670.
OMAWNIFLQP.
PhylomeDBQ8PU59.
ProtClustDBCLSK568843.

Enzyme and pathway databases

BioCycMMAZ192952:MM2487-MONOMER.

Family and domain databases

HAMAPMF_01350. NDH1_NuoH.
[Tree]
InterProIPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
KOK00337.
PANTHERPTHR11432. Resp_NADH_DH_1. 1 hit.
PfamPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEPS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFPOH_METMA
AccessionPrimary (citable) accession number: Q8PU59
Secondary accession number(s): Q9P9F9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: October 1, 2002
Last modified: December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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