Reviewed, UniProtKB/Swiss-Prot Q8PU59 (FPOH_METMA)

Names and origin

Protein names

Recommended name:
    F(420)H(2) oxidoreductase subunit H
Alternative name(s):
    F(420)H(2) dehydrogenase subunit H
    FPO subunit H

Gene names

Name:	fpoH
Ordered Locus Names:	MM_2487

Organism

Methanosarcina mazei (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

2209 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	FPO shuttles electrons from F(420)H2, via FAD and iron-sulfur (Fe-S) centers, to quinones in the F(420)H2:heterodisulfide oxidoreduction chain. The immediate electron acceptor for the enzyme in this species is believed to be methanophenazine. Couples the redox reaction to proton translocation (for every two electrons transferred, 0.9 hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Subunit structure	FPO is composed of at least 12 different subunits. Subunits fpoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	FAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	F(420)H(2) oxidoreductase subunit H HAMAP MF_01350		PRO_0000240123
Regions
	Transmembrane	21 – 41	21	Potential
	Transmembrane	94 – 114	21	Potential
	Transmembrane	128 – 148	21	Potential
	Transmembrane	173 – 193	21	Potential
	Transmembrane	200 – 220	21	Potential
	Transmembrane	261 – 281	21	Potential
	Transmembrane	285 – 305	21	Potential
	Transmembrane	330 – 350	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8PU59-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 21F9E161058612A6	FASTA	350	38,443
	10 20 30 40 50 60 MTFMAIEIPE FIVPFVPWIR GTVGLVLVGA IFLGGMAAVW IERKLSADIQ LRYGPSRVGK 70 80 90 100 110 120 FGLLQLVADA IKLFTKEDVR PGNADRFLYD NAPVFMLTSL FLMLVAIPVG AVFIDGNLYP 130 140 150 160 170 180 LAVTEMDISI LFIEAVSAIN IFGIFMAAYG SNNKYSLLGA FRNFARMIGY EVPLGIAIVS 190 200 210 220 230 240 VAVMTGSLNI IDITSAQGSF VWNIFLQPIG FVVFFIALMA DLGRLPFDQN ESEEELVAGW 250 260 270 280 290 300 VTEYTGMRFG LVFFAEYMHM ILGSFLVALL FLGGWNVPAF VANNAVLGLI APTGILLLKT 310 320 330 340 350 VLVLMTIIGM RWAVPRFRID QVVDMSWKKL LPLSLLNLAW AVGLGLYLGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The F(420)H(2) dehydrogenase from Methanosarcina mazei is a redox-driven proton pump closely related to NADH dehydrogenases." Baeumer S., Ide T., Jacobi C., Johann A., Gottschalk G., Deppenmeier U. J. Biol. Chem. 275:17968-17973(2000) [PubMed: 10751389] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, FPO COMPLEX. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.
[2]	"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.
[3]	"Purification and properties of an F(420)H(2) dehydrogenase from Methanosarcina mazei Go1." Abken H.-J., Deppenmeier U. FEMS Microbiol. Lett. 154:231-237(1997) Cited for: FUNCTION, FPO COMPLEX, CATALYTIC ACTIVITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF228525 Genomic DNA. Translation: AAF65735.1. Different initiation. AE008384 Genomic DNA. Translation: AAM32183.1.
RefSeq	NP_634511.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1480829.
GenomeReviews	Gene locus MM_2487 in contig AE008384_GR.
KEGG	mma:MM_2487.
NMPDR	fig|192952.1.peg.2487.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8PU59.
OMA	Q8PU59. LYLGGWE.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM2487-MON.
Family and domain databases
HAMAP	MF_01350. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FPOH_METMA

Accession

Primary (citable) accession number: Q8PU59
Secondary accession number(s): Q9P9F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)