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Protein

F(420)H(2) dehydrogenase subunit A

Gene

fpoA

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the F(420)H2 dehydrogenase (FPO complex) which is part of the energy-conserving F(420)H2:heterodisulfide oxidoreductase system. The membrane-bound electron transfer system of the complex plays an important role in the metabolism of methylotrophic methanogens when the organisms grow on methanol or methylamines. Catalyzes the oxidation of methanophenazine to dihydromethanophenazine. It shuttles electrons from F(420)H2, via FAD and iron-sulfur (Fe-S) centers, to methanophenazine (an electron carrier in the membrane). It couples the redox reaction to proton translocation (for every two electrons transferred, two hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. It also catalyzes the oxidation of F(420)H2 with quinones such as 2,3-dimethyl-1,4-naphthoquinone, 2-methyl-1,4-naphthoquinone and tetramethyl-p-benzoquinone.2 Publications

Catalytic activityi

H2 + 2-(2,3-dihydropentaprenyloxy)phenazine = 2-dihydropentaprenyloxyphenazine.1 Publication

Kineticsi

Measured for the whole complex.
  1. KM=7 µM for F(420)H2 (at 37 degrees Celsius and pH 7)1 Publication
  1. Vmax=17 µmol/min/mg enzyme (at 37 degrees Celsius and pH 7)1 Publication

pH dependencei

Optimum pH is 8.5.1 Publication

Temperature dependencei

Optimum temperature is 39 degrees Celsius.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Methanogenesis, Methanol utilization, Transport

Enzyme and pathway databases

BRENDAi1.12.98.3 3270

Protein family/group databases

TCDBi3.D.9.1.1 the h(+)-translocating f420h2 dehydrogenase (f420h2dh) family

Names & Taxonomyi

Protein namesi
Recommended name:
F(420)H(2) dehydrogenase subunit A (EC:1.12.98.3)
Alternative name(s):
F(420)H(2)-dependent phenazine dehydrogenase subunit A
F(420)H(2)-dependent phenazine oxidoreductase subunit A
Short name:
FPO subunit A
Methanophenazine hydrogenase subunit A
Methanosarcina-phenazine hydrogenase subunit A
Gene namesi
Name:fpoA
Ordered Locus Names:MM_2491
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Transmembranei64 – 84HelicalSequence analysisAdd BLAST21
Transmembranei95 – 115HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004239541 – 129F(420)H(2) dehydrogenase subunit AAdd BLAST129

Interactioni

Subunit structurei

The FPO complex is composed of at least 13 different subunits. FpoA, FpoH, FpoJ, FpoK, FpoL, FpoM and FpoN proteins constitute the membrane sector of the complex.1 Publication

Protein-protein interaction databases

STRINGi192952.MM_2491

Structurei

3D structure databases

SMRiQ8PU58
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiarCOG01557 Archaea
COG0838 LUCA
HOGENOMiHOG000100120
KOiK22158
OMAiAYEWRRG
OrthoDBiPOG093Z0F6J

Family and domain databases

Gene3Di1.20.58.1610, 1 hit
HAMAPiMF_01394 NDH1_NuoA, 1 hit
InterProiView protein in InterPro
IPR023043 NAD(P)H_OxRDtase_bac/plastid
IPR000440 NADH_UbQ/plastoQ_OxRdtase_su3
IPR038430 NDAH_ubi_oxred_su3_sf
PANTHERiPTHR11058 PTHR11058, 1 hit
PfamiView protein in Pfam
PF00507 Oxidored_q4, 1 hit

Sequencei

Sequence statusi: Complete.

Q8PU58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGDTMSGII DSYIPVAIFL AVGLIMPPMT MFMVKQLSPR SKAASKYTTY
60 70 80 90 100
ESGSVPTGTA RIQFNVEYYL YAIAFVLFDI EVLFLYPWAT VYKGHGITSI
110 120
AVVEMFVFIF ILLFGYVYLW KKEALTWVK
Length:129
Mass (Da):14,667
Last modified:October 1, 2002 - v1
Checksum:iA9EB154E4D37713A
GO

Sequence cautioni

The sequence AAF65731 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF228525 Genomic DNA Translation: AAF65731.1 Different initiation.
AE008384 Genomic DNA Translation: AAM32187.1
RefSeqiWP_015412578.1, NC_003901.1

Genome annotation databases

EnsemblBacteriaiAAM32187; AAM32187; MM_2491
GeneIDi24882214
KEGGimma:MM_2491
PATRICifig|192952.21.peg.2850

Similar proteinsi

Entry informationi

Entry nameiFPOA_METMA
AccessioniPrimary (citable) accession number: Q8PU58
Secondary accession number(s): Q9P9G3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health