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Protein

F(420)H(2) dehydrogenase subunit A

Gene

fpoA

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the F(420)H2 dehydrogenase (FPO complex) which is part of the energy-conserving F(420)H2:heterodisulfide oxidoreductase system. The membrane-bound electron transfer system of the complex plays an important role in the metabolism of methylotrophic methanogens when the organisms grow on methanol or methylamines. Catalyzes the oxidation of methanophenazine to dihydromethanophenazine. It shuttles electrons from F(420)H2, via FAD and iron-sulfur (Fe-S) centers, to methanophenazine (an electron carrier in the membrane). It couples the redox reaction to proton translocation (for every two electrons transferred, two hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. It also catalyzes the oxidation of F(420)H2 with quinones such as 2,3-dimethyl-1,4-naphthoquinone, 2-methyl-1,4-naphthoquinone and tetramethyl-p-benzoquinone.2 Publications

Catalytic activityi

H2 + 2-(2,3-dihydropentaprenyloxy)phenazine = 2-dihydropentaprenyloxyphenazine.1 Publication

Kineticsi

Measured for the whole complex.

  1. KM=7 µM for F(420)H2 (at 37 degrees Celsius and pH 7)1 Publication
  1. Vmax=17 µmol/min/mg enzyme (at 37 degrees Celsius and pH 7)1 Publication

pH dependencei

Optimum pH is 8.5.1 Publication

Temperature dependencei

Optimum temperature is 39 degrees Celsius.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Methanogenesis, Methanol utilization, Transport

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-2549-MONOMER.
BRENDAi1.12.98.3. 3270.

Protein family/group databases

TCDBi3.D.9.1.1. the h(+)-translocating f420h2 dehydrogenase (f420h2dh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
F(420)H(2) dehydrogenase subunit A (EC:1.12.98.3)
Alternative name(s):
F(420)H(2)-dependent phenazine dehydrogenase subunit A
F(420)H(2)-dependent phenazine oxidoreductase subunit A
Short name:
FPO subunit A
Methanophenazine hydrogenase subunit A
Methanosarcina-phenazine hydrogenase subunit A
Gene namesi
Name:fpoA
Ordered Locus Names:MM_2491
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Transmembranei64 – 8421HelicalSequence analysisAdd
BLAST
Transmembranei95 – 11521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 129129F(420)H(2) dehydrogenase subunit APRO_0000423954Add
BLAST

Interactioni

Subunit structurei

The FPO complex is composed of at least 13 different subunits. FpoA, FpoH, FpoJ, FpoK, FpoL, FpoM and FpoN proteins constitute the membrane sector of the complex.1 Publication

Protein-protein interaction databases

STRINGi192952.MM_2491.

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiarCOG01557. Archaea.
COG0838. LUCA.
HOGENOMiHOG000100120.
KOiK00330.
OMAiMKGALEW.

Family and domain databases

HAMAPiMF_01394. NDH1_NuoA. 1 hit.
InterProiIPR023043. NAD(P)H_OxRDtase_bac/plastid.
IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3.
[Graphical view]
PANTHERiPTHR11058. PTHR11058. 1 hit.
PfamiPF00507. Oxidored_q4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8PU58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGDTMSGII DSYIPVAIFL AVGLIMPPMT MFMVKQLSPR SKAASKYTTY
60 70 80 90 100
ESGSVPTGTA RIQFNVEYYL YAIAFVLFDI EVLFLYPWAT VYKGHGITSI
110 120
AVVEMFVFIF ILLFGYVYLW KKEALTWVK
Length:129
Mass (Da):14,667
Last modified:October 1, 2002 - v1
Checksum:iA9EB154E4D37713A
GO

Sequence cautioni

The sequence AAF65731 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF228525 Genomic DNA. Translation: AAF65731.1. Different initiation.
AE008384 Genomic DNA. Translation: AAM32187.1.

Genome annotation databases

EnsemblBacteriaiAAM32187; AAM32187; MM_2491.
KEGGimma:MM_2491.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF228525 Genomic DNA. Translation: AAF65731.1. Different initiation.
AE008384 Genomic DNA. Translation: AAM32187.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_2491.

Protein family/group databases

TCDBi3.D.9.1.1. the h(+)-translocating f420h2 dehydrogenase (f420h2dh) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM32187; AAM32187; MM_2491.
KEGGimma:MM_2491.

Phylogenomic databases

eggNOGiarCOG01557. Archaea.
COG0838. LUCA.
HOGENOMiHOG000100120.
KOiK00330.
OMAiMKGALEW.

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-2549-MONOMER.
BRENDAi1.12.98.3. 3270.

Family and domain databases

HAMAPiMF_01394. NDH1_NuoA. 1 hit.
InterProiIPR023043. NAD(P)H_OxRDtase_bac/plastid.
IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3.
[Graphical view]
PANTHERiPTHR11058. PTHR11058. 1 hit.
PfamiPF00507. Oxidored_q4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFPOA_METMA
AccessioniPrimary (citable) accession number: Q8PU58
Secondary accession number(s): Q9P9G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: October 1, 2002
Last modified: November 11, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.