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Protein

2-phospho-L-lactate guanylyltransferase

Gene

cofC

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Guanylyltransferase that catalyzes the activation of 2-phospho-L-lactate (LP) as (2S)-lactyl-2-diphospho-5'-guanosine (LPPG), via the condensation of LP with GTP. Is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor.UniRule annotation

Catalytic activityi

(2S)-2-phospholactate + GTP = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate.UniRule annotation

Pathwayi: coenzyme F420 biosynthesis

This protein is involved in the pathway coenzyme F420 biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway coenzyme F420 biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
2-phospho-L-lactate guanylyltransferaseUniRule annotation (EC:2.7.7.68UniRule annotation)
Short name:
LP guanylyltransferaseUniRule annotation
Gene namesi
Name:cofCUniRule annotation
Ordered Locus Names:MM_2497
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003987571 – 2082-phospho-L-lactate guanylyltransferaseAdd BLAST208

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi192952.MM_2497.

Structurei

Secondary structure

1208
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Turni10 – 13Combined sources4
Helixi14 – 16Combined sources3
Turni17 – 19Combined sources3
Helixi22 – 42Combined sources21
Beta strandi48 – 54Combined sources7
Beta strandi63 – 68Combined sources6
Helixi73 – 83Combined sources11
Beta strandi88 – 91Combined sources4
Helixi100 – 106Combined sources7
Beta strandi110 – 117Combined sources8
Helixi119 – 121Combined sources3
Beta strandi123 – 129Combined sources7
Helixi131 – 133Combined sources3
Beta strandi139 – 141Combined sources3
Helixi142 – 151Combined sources10
Turni152 – 154Combined sources3
Beta strandi157 – 159Combined sources3
Turni163 – 166Combined sources4
Helixi172 – 181Combined sources10
Helixi185 – 191Combined sources7
Beta strandi194 – 198Combined sources5
Beta strandi200 – 207Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I5EX-ray2.10A/B1-208[»]
ProteinModelPortaliQ8PU52.
SMRiQ8PU52.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8PU52.

Family & Domainsi

Sequence similaritiesi

Belongs to the CofC family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04472. Archaea.
COG1920. LUCA.
HOGENOMiHOG000223639.
KOiK14941.
OMAiRAVIPYK.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_02114. CofC. 1 hit.
InterProiIPR002835. Coenzyme_F420_CofC.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01983. CofC. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03552. F420_cofC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8PU52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAVIPYKKA GAKSRLSPVL SLQEREEFVE LMLNQVISSL KGAGIEQVDI
60 70 80 90 100
LSPSVYGLEE MTEARVLLDE KDLNEALNRY LKEAEEPVLI VMADLPLLSP
110 120 130 140 150
EHIKEISSTE KDVCIVPGKG GGTNALFIKN PSKYRVKYYG SSFLTHCSIA
160 170 180 190 200
TDSGQDFEIY DSFMAGTDID EPEDLVELLI HGKGAAKDYI ESKFRLEVKK

GRVGLVPL
Length:208
Mass (Da):23,051
Last modified:October 1, 2002 - v1
Checksum:iFBD850747C2D769B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM32193.1.
RefSeqiWP_011034415.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM32193; AAM32193; MM_2497.
GeneIDi24882221.
KEGGimma:MM_2497.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM32193.1.
RefSeqiWP_011034415.1. NC_003901.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I5EX-ray2.10A/B1-208[»]
ProteinModelPortaliQ8PU52.
SMRiQ8PU52.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_2497.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM32193; AAM32193; MM_2497.
GeneIDi24882221.
KEGGimma:MM_2497.

Phylogenomic databases

eggNOGiarCOG04472. Archaea.
COG1920. LUCA.
HOGENOMiHOG000223639.
KOiK14941.
OMAiRAVIPYK.

Enzyme and pathway databases

UniPathwayiUPA00071.

Miscellaneous databases

EvolutionaryTraceiQ8PU52.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_02114. CofC. 1 hit.
InterProiIPR002835. Coenzyme_F420_CofC.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01983. CofC. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03552. F420_cofC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOFC_METMA
AccessioniPrimary (citable) accession number: Q8PU52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.