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Protein

2-phospho-L-lactate guanylyltransferase

Gene

cofC

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Guanylyltransferase that catalyzes the activation of 2-phospho-L-lactate (LP) as (2S)-lactyl-2-diphospho-5'-guanosine (LPPG), via the condensation of LP with GTP. Is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor.UniRule annotation

Catalytic activityi

(2S)-2-phospholactate + GTP = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate.UniRule annotation

Pathwayi: coenzyme F420 biosynthesis

This protein is involved in the pathway coenzyme F420 biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway coenzyme F420 biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-2555-MONOMER.
UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
2-phospho-L-lactate guanylyltransferaseUniRule annotation (EC:2.7.7.68UniRule annotation)
Short name:
LP guanylyltransferaseUniRule annotation
Gene namesi
Name:cofCUniRule annotation
Ordered Locus Names:MM_2497
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000000595 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2082082-phospho-L-lactate guanylyltransferasePRO_0000398757Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi192952.MM_2497.

Structurei

Secondary structure

1
208
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Turni10 – 134Combined sources
Helixi14 – 163Combined sources
Turni17 – 193Combined sources
Helixi22 – 4221Combined sources
Beta strandi48 – 547Combined sources
Beta strandi63 – 686Combined sources
Helixi73 – 8311Combined sources
Beta strandi88 – 914Combined sources
Helixi100 – 1067Combined sources
Beta strandi110 – 1178Combined sources
Helixi119 – 1213Combined sources
Beta strandi123 – 1297Combined sources
Helixi131 – 1333Combined sources
Beta strandi139 – 1413Combined sources
Helixi142 – 15110Combined sources
Turni152 – 1543Combined sources
Beta strandi157 – 1593Combined sources
Turni163 – 1664Combined sources
Helixi172 – 18110Combined sources
Helixi185 – 1917Combined sources
Beta strandi194 – 1985Combined sources
Beta strandi200 – 2078Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I5EX-ray2.10A/B1-208[»]
ProteinModelPortaliQ8PU52.
SMRiQ8PU52. Positions 1-208.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8PU52.

Family & Domainsi

Sequence similaritiesi

Belongs to the CofC family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04472. Archaea.
COG1920. LUCA.
HOGENOMiHOG000223639.
KOiK14941.
OMAiRAVIPYK.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_02114. CofC. 1 hit.
InterProiIPR002835. Coenzyme_F420_CofC.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01983. CofC. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03552. F420_cofC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8PU52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAVIPYKKA GAKSRLSPVL SLQEREEFVE LMLNQVISSL KGAGIEQVDI
60 70 80 90 100
LSPSVYGLEE MTEARVLLDE KDLNEALNRY LKEAEEPVLI VMADLPLLSP
110 120 130 140 150
EHIKEISSTE KDVCIVPGKG GGTNALFIKN PSKYRVKYYG SSFLTHCSIA
160 170 180 190 200
TDSGQDFEIY DSFMAGTDID EPEDLVELLI HGKGAAKDYI ESKFRLEVKK

GRVGLVPL
Length:208
Mass (Da):23,051
Last modified:October 1, 2002 - v1
Checksum:iFBD850747C2D769B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM32193.1.
RefSeqiWP_011034415.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM32193; AAM32193; MM_2497.
GeneIDi24882221.
KEGGimma:MM_2497.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008384 Genomic DNA. Translation: AAM32193.1.
RefSeqiWP_011034415.1. NC_003901.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I5EX-ray2.10A/B1-208[»]
ProteinModelPortaliQ8PU52.
SMRiQ8PU52. Positions 1-208.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192952.MM_2497.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM32193; AAM32193; MM_2497.
GeneIDi24882221.
KEGGimma:MM_2497.

Phylogenomic databases

eggNOGiarCOG04472. Archaea.
COG1920. LUCA.
HOGENOMiHOG000223639.
KOiK14941.
OMAiRAVIPYK.

Enzyme and pathway databases

UniPathwayiUPA00071.
BioCyciMMAZ192952:GCK2-2555-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ8PU52.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_02114. CofC. 1 hit.
InterProiIPR002835. Coenzyme_F420_CofC.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01983. CofC. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03552. F420_cofC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOFC_METMA
AccessioniPrimary (citable) accession number: Q8PU52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 1, 2002
Last modified: December 9, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.