ID   TRM1_METMA              Reviewed;         388 AA.
AC   Q8PU28;
DT   10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   10-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 38.
DE   RecName: Full=N(2),N(2)-dimethylguanosine tRNA methyltransferase;
DE            EC=2.1.1.32;
DE   AltName: Full=tRNA(guanine-26,N(2)-N(2)) methyltransferase;
DE   AltName: Full=tRNA 2,2-dimethylguanosine-26 methyltransferase;
DE   AltName: Full=tRNA(m(2,2)G26)dimethyltransferase;
GN   Name=trm1; OrderedLocusNames=MM_2528;
OS   Methanosarcina mazei (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales;
OC   Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=2209;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88;
RX   MEDLINE=22120827; PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene
RT   transfer between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
CC   -!- FUNCTION: Dimethylates a single guanine residue at position 26 of
CC       a number of tRNAs using S-adenosyl-L-methionine as donor of the
CC       methyl groups (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-
CC       homocysteine + tRNA containing N(2)-methylguanine.
CC   -!- SIMILARITY: Belongs to the TRM1 family.
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DR   EMBL; AE008384; AAM32224.1; -; Genomic_DNA.
DR   RefSeq; NP_634552.1; -.
DR   GeneID; 1480870; -.
DR   GenomeReviews; AE008384_GR; MM_2528.
DR   KEGG; mma:MM_2528; -.
DR   NMPDR; fig|192952.1.peg.2528; -.
DR   HOGENOM; Q8PU28; -.
DR   OMA; Q8PU28; MELNRDI.
DR   BioCyc; MMAZ192952:MM2528-MON; -.
DR   BRENDA; 2.1.1.32; 261165.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0004809; F:tRNA (guanine-N2-)-methyltransferase activity; IEA:HAMAP.
DR   GO; GO:0006400; P:tRNA modification; IEA:HAMAP.
DR   HAMAP; MF_00290; -; 1.
DR   InterPro; IPR002905; TRM_MeTrfase.
DR   PANTHER; PTHR10631; TRM_mtfrase; 1.
DR   Pfam; PF02005; TRM; 1.
DR   TIGRFAMs; TIGR00308; TRM1; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN         1    388       N(2),N(2)-dimethylguanosine tRNA
FT                                methyltransferase.
FT                                /FTId=PRO_0000147684.
SQ   SEQUENCE   388 AA;  42784 MW;  F3796A5EC7820DD3 CRC64;
     MICRTIVEGT TKISVPVPPP DANFPPSAAP VFYNPEMELN RDINVAATAA FVERLLSKKD
     ILREEIRYVD AFSASGIRGL RIAGEVGIHS TMNDWSHEAF ELIKENIKIN GLEEKAQATR
     RNANVLLHEQ RFHIVDVDPF GTPSPYLDAA ASSAYSMLSV TATDTAPLCG AHLNSGIRKY
     ASVPLNTEYH SEMGLRVLLG ACARELAKHE KGMLPLLSHV TRHYVRTYLE VLPGSRKADK
     TLKSMGFVAH CPRCGFRKPV YGLAVHIEKE CPECGGLTKI AGPLWLGPYR EPEFCNEVIS
     ELEAHPLNTK EKVRKIITFC RDELDIPMFY DQHVICKELG ASATGIESLI EALRAGGFEA
     SRTHFTGTSF KTDAPIAEIK KIILALSG
//