Reviewed, UniProtKB/Swiss-Prot Q8PU28 (TRM1_METMA)

Names and origin

Protein names

Recommended name:
    N(2),N(2)-dimethylguanosine tRNA methyltransferase
    EC=2.1.1.32
Alternative name(s):
    tRNA(guanine-26,N(2)-N(2)) methyltransferase
    tRNA 2,2-dimethylguanosine-26 methyltransferase
    tRNA(m(2,2)G26)dimethyltransferase

Gene names

Name:	trm1
Ordered Locus Names:	MM_2528

Organism

Methanosarcina mazei (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

2209 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(2)-methylguanine. HAMAP MF_00290
Sequence similarities	Belongs to the TRM1 family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	N(2),N(2)-dimethylguanosine tRNA methyltransferase HAMAP MF_00290		PRO_0000147684

Sequences

	Sequence		Length	Mass (Da)
	Q8PU28-1 [UniParc]. Last modified October 10, 2002. Version 1. Checksum: F3796A5EC7820DD3	FASTA	388	42,784
	10 20 30 40 50 60 MICRTIVEGT TKISVPVPPP DANFPPSAAP VFYNPEMELN RDINVAATAA FVERLLSKKD 70 80 90 100 110 120 ILREEIRYVD AFSASGIRGL RIAGEVGIHS TMNDWSHEAF ELIKENIKIN GLEEKAQATR 130 140 150 160 170 180 RNANVLLHEQ RFHIVDVDPF GTPSPYLDAA ASSAYSMLSV TATDTAPLCG AHLNSGIRKY 190 200 210 220 230 240 ASVPLNTEYH SEMGLRVLLG ACARELAKHE KGMLPLLSHV TRHYVRTYLE VLPGSRKADK 250 260 270 280 290 300 TLKSMGFVAH CPRCGFRKPV YGLAVHIEKE CPECGGLTKI AGPLWLGPYR EPEFCNEVIS 310 320 330 340 350 360 ELEAHPLNTK EKVRKIITFC RDELDIPMFY DQHVICKELG ASATGIESLI EALRAGGFEA 370 380 SRTHFTGTSF KTDAPIAEIK KIILALSG

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008384 Genomic DNA. Translation: AAM32224.1.
RefSeq	NP_634552.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1480870.
GenomeReviews	Gene locus MM_2528 in contig AE008384_GR.
KEGG	mma:MM_2528.
NMPDR	fig|192952.1.peg.2528.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8PU28.
OMA	Q8PU28. MELNRDI.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM2528-MON.
BRENDA	2.1.1.32. 261165.
Family and domain databases
HAMAP	MF_00290. [Tree]
InterPro	IPR002905. TRM_MeTrfase. [Graphical view]
PANTHER	PTHR10631. TRM_mtfrase. 1 hit.
Pfam	PF02005. TRM. 1 hit. [Graphical view]
TIGRFAMs	TIGR00308. TRM1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRM1_METMA

Accession

Primary (citable) accession number: Q8PU28

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	October 10, 2002
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)